Acetyl-l-carnitine (ALCAR) for the prevention of chemotherapy-induced peripheral neuropathy in patients with relapsed or refractory multiple myeloma treated with bortezomib, doxorubicin and low-dose dexamethasone: a study from the Wisconsin Oncology Network

Multiple myeloma is an incurable plasma cell malignancy with a course characterized by initial responsiveness to treatment, followed by the appearance of increasingly refractory disease and ultimately death due to infection, renal failure and cytopenias [1]. Until the last two decades, patients with MM had few therapeutic options after failing initial therapy with oral alkylators and steroids. Bortezomib, the first clinically approved proteasome inhibitor proved to be a powerful new agent for the treatment of MM [2]. Initial trials of single agent bortezomib for relapsed MM resulted in response rates of 30–35 % [3, 4]. However, MM patients rapidly acquire resistance to bortezomib when used as a single agent, and it is apparent that this resistance may in part be modified through combination with other existing chemotherapy agents, including steroids, alkylating agents, anthracyclines and immunomodulatory drugs [5‐7]. As more experience has been gained with these combinations, it is also apparent that some patients who have previously received bortezomib can be retreated at the time of relapse with excellent results [8]. However, predicting which relapsed patients will respond to retreatment with bortezomib remains essentially a “trial and error” process.

An additional obstacle when considering retreatment of MM patients has been the rapid development of peripheral sensory and occasionally motor neuropathy that is the most frequently observed important non-hematologic toxicity of bortezomib. The exact mechanisms underlying bortezomib-induced PN remain unclear. Animal models of bortezomib-induced PN demonstrate damage to dorsal root ganglia (DRG) neuronal cell bodies including chromatolysis and accumulation of electron dense juxtanuclear cytoplasmic deposits [9]. Casafont et al. [10] demonstrated accumulation of poly (A) RNAs in nuclear granules and suggested that interference with pre-mRNA processing may be a major pathologic event in the development of PN. The incidence and severity of peripheral neuropathy (PN) is influenced by the schedule used (e.g., twice weekly versus weekly [11]), the route of administration (intravenous push versus subcutaneous [12]), and concurrent administration of other chemotherapeutic agents. In particular, combining bortezomib with anthracyclines produces some of the highest rates and severity of PN [13, 14]. The presence of certain genetic polymorphisms may also influence the incidence and severity of bortezomib-related PN [15, 16].

In an effort to ameliorate peripheral neuropathy, some investigators have suggested the addition of various agents thought to be neuroprotective at the time PN emerges. For example Richardson et al. [17] in the initial phase II study of bortezomib for subjects with newly diagnosed myeloma, offered patients gabapentin as well as other supplements at the emergence of ≥grade 1 neuropathy. Anecdotal reports of improvement of PN have also been attributed to the use of a number of supplements, including alpha lipoic acid [18], and B vitamins including pyridoxine [19] and vitamin B12 [20].

Acetyl-l-carnitine (ALCAR), another compound of interest, is an ester of l-carnitine and a critical component in mitochondrial energetics and function. ALCAR appears to act as a transport molecule for fatty acids in and out of the axonal mitochondria, where they are then utilized in energy-producing metabolic cycles. In animal models of chemotherapy-induced PN, ALCAR, administered to rats dosed with paclitaxel, vincristine, oxaliplatin, or cisplatin, decreased the incidence of allodynia (the sensation of pain in response to a normal stimuli) [21‐24]. It is not yet clear whether the benefits noted from ALCAR are due to the intact ester or to the combined effect of the acetyl and l-carnitine moieties formed by hydrolysis of the parent molecule. Other attractive features of ALCAR are that it is extremely well tolerated, has no known drug interactions, comes as an oral preparation and is available without prescription as a dietary supplement. Studies in human subjects have suggested that ALCAR may be effective in treating patients with chemotherapy-induced NP in two uncontrolled, pilot trials [25, 26]. We therefore investigated the ability of ALCAR to prevent the emergence of bortezomib-induced PN in a two part pilot study using the combination of bortezomib, doxorubicin and dexamethasone in a heavily pretreated group of patients. We specifically allowed enrollment of patients refractory to the bortezomib and dexamethasone doublet. In the first half of the study patients received the three-drug combination without ALCAR to examine the response rate and toxicities. Although seemingly an active combination, we noted a high incidence of PN that affected the ability to deliver subsequent cycles of therapy. Therefore, patients accrued to the second part of the study received prophylactic ALCAR to see if the incidence and severity of PN would be reduced and to determine if response rates were maintained when ALCAR was included.

As an additional exploratory component of the study, we also sought to determine if we could predict an individual subject’s response to the BDD combination by examining the inherent NF-κB activation status of the patient’s primary myeloma cells as this transcription factor activity has been implicated in promoting survival of cancer cells. The biological mechanisms underlying primary or acquired bortezomib resistance are not known although the development of structural variants in the β5-subunit of the 20S proteasome core has been cited [27]. Previously our group has demonstrated that a subset of primary MM cells isolated from patient bone marrow samples display enhanced constitutive NF-κB activity when exposed to bortezomib and such activation in MM cell lines correlated with bortezomib-resistance in vitro [28]. We therefore sought to evaluate in a subset of patients receiving treatment on this trial whether we could correlate clinical response with this phenomenon. We hypothesized that MM cells from responding subjects would be less likely to show bortezomib enhanced NF-κB activity and that non-responders would display bortezomib-inducible NF-κB activity.

Patients with relapsed and/or refractory MM, including those relapsing on bortezomib, were eligible. Progressive/relapsed disease was defined as an increase of >1 g/dl of monoclonal protein, or >200 mg of protein in a 24-h urine collection; those subjects who progressed thusly while on or within 60 days of treatment were deemed refractory. Patients were informed of the investigational nature of the study and signed informed consent. The study was conducted at all Wisconsin Oncology Network sites after appropriate approval by individual institutional review boards in compliance with the Declaration of Helsinki and Good Clinical Practice Guidelines. Patients with preexisting >grade 2 peripheral motor or sensory neuropathy were excluded. Minimum laboratory requirements included absolute neutrophils >1,500/mcl and platelets >100,000/mcl (unless the cytopenias were due to marrow replacement with MM), ALT and AST < 3 times the institutional upper limits, no more than 220 mg/m² of previous doxorubicin exposure and left ventricular ejection fraction >40 % as determined by echocardiogram or MUGA within the previous 90 days. Prior to treatment, patients were staged with serum protein electrophoresis, 24-h urine collection, skeletal survey and bone marrow biopsy.

Treatment consisted of bortezomib (B) 1.3 mg/m² on day 1, 4, 8 and 11 intravenously, doxorubicin (D) 15 mg/m² on days 1 and 8 intravenously and dexamethasone (Dex) 20 mg by mouth on days 1, 4, 8 and 11 for up to 8 cycles. Prophylactic acyclovir was required to prevent varicella reactivation. Dose reductions of B and D were used for cytopenias and treatment emergent PN. Growth factor use was allowed and encouraged per investigator discretion to maintain the dosing schedule.

As part of the neuroprotection portion of the trial, subjects received the identical chemotherapy regimen but in addition received ALCAR 1.5 g by mouth twice daily. These patients answered the FACT-GOG-NTX and FACIT-Fatigue [29] questionnaires at the time of enrollment, and prior to each odd cycle. A member of the research team administered the Grooved Peg Board test [30] in duplicate. Patients in either cohort achieving a complete remission (CR) underwent a bone marrow biopsy at that time to confirm response. CR was defined as no monoclonal protein detectable by immunofixation of serum and urine as well as <5 % plasma cells in the marrow. Partial response (PR) was defined as >50 % reduction in serum monoclonal protein, minimal response (MR) between 25 and 50 % reduction and progressive disease as at least 25 % increase in serum M protein (minimum >0.5 g/dl) or 200 mg in urine protein from the lowest level, new lytic bone lesions, or hypercalcemia. Responses were evaluated every two cycles.

Bortezomib is an extremely active agent in relapsed MM patients, especially when combined with steroids and other compounds such as alkylating agents. In particular, numerous reports attest to the utility of retreatment with bortezomib. We have demonstrated that the combination of doxorubicin, low-dose dexamethasone and bortezomib is associated with a high response rate of 53 % (CR and PR), even in very refractory patients. This observed response rate is similar to that reported by Palumbo et al. [32] using a different dosing schedule of bortezomib, doxorubicin and dexamethasone. They found an overall response rate in 67 % of patients, although an important difference is that in their trial, greater than 60 % of patients received the three-drug bortezomib-based combination as their first- or second-line relapse therapy. However, both preexisting and treatment emergent neuropathy continues to be an important consideration that limits long-term administration of bortezomib. We attempted to mitigate the incidence and severity of PN through the use of prophylactic acetyl-l-carnitine. Our study suggests that the addition of ALCAR did not eliminate treatment-related PN, although there appeared to be fewer cases of grade 3 or 4 neuropathy among patients receiving the prophylaxis as reported by the treating physicians. However, as measured by validated instruments such as the FACIT-GOG-NTX and the NPI index, the subjects reported increasing levels of neuropathy and continuing fatigue as they continued on study. Given the observed continued high responses to the BDD-A combination, it is clear that the inclusion of this agent in the treatment regimen did not diminish the response rate and ALCAR was very well tolerated. Major limitations of our study include the study’s small subject numbers and that we did not assess subjects receiving BDD with the same instruments, relying on PN assessment by study personnel using CTCAE criteria. Of note, in their BDD combination study, Palumbo reported only a 10 % incidence of grade 3–4 treatment emergent PN, substantially lower than the 25 % noted for our entire study. An important factor may be that the preexisting prevalence of PN in their subjects was 22 % compared to our subjects at 34 %.

Initial trials incorporating bortezomib quickly pointed to PN as an important toxicity of this drug [32, 33]. Other trials incorporating bortezomib in previously treated patients have reported similar rates of PN and grade 3–4 toxicity. Orlowski et al. [34] in the pivotal trial of pegylated doxorubicin and bortezomib reported an 80 % incidence of ≥grade 3 adverse events (AE): 36 % of patients stopped therapy due to an AE. The incidence of PN was reported as 35 % with only 4 % reported as grade 3/4. Richardson et al. [16] published data from the SUMMIT and CREST trial of bortezomib and dexamethasone in relapsed MM patients showing that 80 % of subjects either reported PN or were clinically assessed as having PN. In our much smaller study, we found that only 55 % of these heavily pretreated subjects reported significant PN but some of this difference may be due to the lower rate of exposure to thalidomide prior to enrollment (51 %) compared to subjects in those trials (72 %). Our observed rate of treatment emergent PN was also higher than that reported in either the SUMMIT [3] or CREST [4] trials. Newer methods of bortezomib dosing, such as subcutaneous or weekly administrations, appear to significantly lower the rate of PN, but it remains an important issue [11, 12, 35]. These modifications would seem the current best approach in minimizing bortezomib-related PN as the preliminary data from our small study does not support any advantage for the inclusion of ALCAR in this clinical setting. ALCAR administered intramuscularly has been shown in a randomized double-blind trial to improve retroviral therapy-induced PN [36] and in a small series of patients on RT receiving long-term oral ALCAR [37]. It is therefore conceivable that the lack of protective effect in our trial may be due to different mechanisms of neuronal injury caused by bortezomib. It is also conceivable that the incorporation of ALCAR in bortezomib containing regimens earlier in the treatment course, e.g., for newly diagnosed MM patients, might offer a protective advantage against the development of PN. However, Hershman recently reported results from a large placebo-controlled, randomized trial in which breast cancer patients receiving adjuvant taxane-based chemotherapy were assigned to ALCAR versus placebo. Their study found no improvement in patient-reported PN and actually showed an increase in PN severity in patients receiving ALCAR as measured by FACT-NTX and clinician assessment after 24 weeks of therapy [38]. Given the scope of this trial, it seems less likely that the reason we also did not observe a reduction in PN with the inclusion of ALCAR was due sample size.

We also examined the pretreatment activation status of NF-κB in primary myeloma cells obtained from bone marrow in a subset of patients. We found a correlation between clinically relevant bortezomib-resistance and bortezomib-inducible NF-κB activation. Despite the use of what is considered an active regimen, there was a distinctly lower response rate among those patients whose primary myeloma cells displayed this characteristic. This bortezomib-inducible activity was found both in subjects with previous exposure to B and in one B-naive subject who failed to respond to BDD. These interesting results raise the possibility of using such an assay at the time of staging bone marrow biopsy to help determine optimal therapy. However, the assay as conducted here requires large numbers of MM cells and we are attempting to modify the assay to allow for use of smaller aliquots of primary MM cells [39]. In the future, we hope this technology could be incorporated in real time to determine the most effective therapeutic options for newly diagnosed and relapsed patients.