Adipocyte-released insulin-like growth factor-1 is regulated by glucose and fatty acids and controls breast cancer cell growth in vitro
verfasst von:
V. D’Esposito, F. Passaretti, A. Hammarstedt, D. Liguoro, D. Terracciano, G. Molea, L. Canta, C. Miele, U. Smith, F. Beguinot, P. Formisano
Type 2 diabetes and obesity are associated with increased risk of site-specific cancers. We have investigated whether metabolic alterations at the level of adipose-derived differentiating cells may affect specific phenotypes of breast cancer cells.
Methods
Growth profiles of breast cancer cell lines were evaluated in co-cultures with differentiated adipocytes or their precursor cells and upon treatment with adipocyte conditioned media. Production and release of cytokines and growth factors were assessed by real-time RT-PCR and multiplex-based ELISA assays.
Results
Co-cultures with either differentiated mouse 3T3-L1 or human mammary adipocytes increased viability of MCF-7 cells to a greater extent, when compared with their undifferentiated precursors. Adipocytes cultured in 25 mmol/l glucose were twofold more effective in promoting cell growth, compared with those grown in 5.5 mmol/l glucose, and activated mitogenic pathways in MCF-7 cells. Growth-promoting action was also enhanced when adipocytes were incubated in the presence of palmitate or oleate. Interestingly, 3T3-L1 and human adipocytes released higher amounts of keratinocyte-derived chemokine/IL-8, the protein ‘regulated upon activation, normally T expressed, and secreted’ (RANTES), and IGF-1, compared with their precursor cells. Their levels were reduced upon incubation with low glucose and enhanced by fatty acids. Moreover, both undifferentiated cells and differentiated adipocytes from obese individuals displayed about twofold higher IGF-1 release and MCF-7 cell growth induction than lean individuals. Finally, inhibition of the IGF-1 pathway almost completely prevented the growth-promoting effect of adipocytes on breast cancer cells.
Conclusions/interpretation
IGF-1 release by adipocytes is regulated by glucose and fatty acids and may contribute to the control of cancer cell growth in obese individuals.
The online version of this article (doi:10.1007/s00125-012-2629-7) contains peer-reviewed but unedited supplementary material, which is available to authorised users.
Abkürzungen
Adipo 3T3-L1
3T3-L1 adipocytes
AU
Arbitrary units
bFGF
Basic fibroblast growth factor
CM
Conditioned medium
ER
Oestrogen (estrogen) receptor
ERK
Extracellular signal-regulated kinase
G-CSF
Granulocyte-colony stimulating factor
GM-CSF
Granulocyte–macrophage colony stimulating factor
hAdipo
Human adipocyte
HG
High glucose
JAK
Janus kinase
KC
Keratinocyte-derived chemokine
LG
Low glucose
MAPK
Mitogen-activated protein kinase
MCP-1
Monocyte chemoattractant protein 1
MIP-1α/β
Macrophage inflammatory protein 1-alpha/beta
OLE
Oleate
PAL
Palmitate
PDGF
Platelet-derived growth factor
PI3K
Phosphatidylinositol 3-kinase
PKB/Akt
Protein kinase B
RANTES
Regulated upon activation normally T expressed, and secreted
STAT
Signal transducer and activator of transcription
SVF
Stromal vascular fraction
TBS
Tris-buffered saline
VEGF
Vascular endothelial growth factor
Introduction
It has recently become clear that obesity and type 2 diabetes are associated with an increased frequency of many cancers [1, 2]. For instance, increased adiposity is associated with an increased risk of several cancers [3]. Similarly, epidemiological data link type 2 diabetes with an increased incidence of multiple types of cancer, including breast, colorectal, hepatocellular, endometrial and pancreatic malignancies [4, 5]. Both obesity and diabetes can also lead to poorer treatment outcome and increase cancer-related mortality rates [6‐8].
The adipocyte may represent a candidate to integrate energy and nutrient metabolism with cancer cell growth by regulating cell functions through a complex network of endocrine, paracrine and autocrine signals [9‐11]. Indeed, in addition to storing excess calories in the form of lipid, adipose tissue is an active endocrine organ that can have far-reaching effects on the physiology of other tissues [12]. It has been shown that several hormones, growth factors and cytokines are produced or generated in white adipose tissue [13]. Recent interest has centred on the role of adipokines, chiefly leptin and adiponectin, and inflammatory cytokines [14]. However, all of the adipocyte factors may be envisioned as contributing factors for cancer onset or progression [15]. Moreover, adipocytes are largely represented in the microenvironment of several tumours, possibly providing a number of signals and resources to tumour cells.
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The adipocyte represents one of the most abundant cell types surrounding breast cancer cells and may prove to be a key player in the stromal–ductal epithelial cell interactions within the mammary microenvironment [16]. In this regard, several in-vitro and in-vivo studies demonstrated that adipocytes can directly influence breast tumour growth [17].
Obesity has been consistently shown to increase the rate of breast cancer occurrence in postmenopausal women by 30–50% [11]. Studies of breast cancer mortality rates and survival illustrate that adiposity is associated both with poorer survival and increased likelihood of recurrence among those with the disease, regardless of menopausal status and after adjustment for disease stage and treatment. Very obese women (BMI ≥40.0) have breast cancer death rates that are three times higher than very lean (BMI < 20.5) women [3, 18]. Moreover, case–control and cohort studies showed that women with diabetes may have a 20% increased risk of breast cancer [19] and increased cancer-related mortality rate [20].
Nevertheless, whether adipocyte-derived factors are directly involved in breast cancer onset or progression is still poorly understood [21]. The effect on tumour phenotype of metabolic alterations at the level of the adipose tissue is also unclear.
Here, we show that adipocytes may integrate inputs from the metabolic environment and promote growth of breast cancer cells. Indeed, both glucose and fatty acids enhance the ability of adipocytes to produce factors, including IL-8, RANTES (regulated upon activation, normally T expressed, and secreted) and IGF-1, involved in the control of cancer cell phenotypes. Finally, both stromal vascular fraction (SVF) cells and differentiated adipocytes from obese individuals release more IGF-1 than those from lean individuals, suggesting that obesity per se may enhance breast cancer cell growth.
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Methods
Materials
Media, sera and antibiotics for cell culture were from Lonza (Basel, Switzerland). Antibodies against phospho-Ser473 protein kinase B (PKB/Akt1), forms of extracellular signal-regulated kinase (ERK), signal transducer and activator of transcription (STAT)3 and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho-Thr202/Tyr204 ERK and phospho-Tyr705 STAT3 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). PKB/Akt antibody was from Millipore (Billerica, MA, USA). Anti-leptin antibodies were a generous gift from G. Matarese (CNR, Naples, Italy). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) reagents were from Bio-Rad (Hercules, CA, USA). All the other chemicals were from Sigma-Aldrich (St Louis, MO, USA).
Cell cultures
MCF-7 (ERα positive) and MDA-MB-231 (ERα negative) human breast cancer cells and 3T3-L1 mouse fibroblasts were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 2 mmol/l glutamine, 100 IU/ml penicillin and 100 IU/ml streptomycin. Cultures were maintained in a humidified atmosphere of 95% air and 5% CO2 at 37°C. 3T3-L1 differentiation was achieved as previously described [22]. Human adipose tissue samples were obtained from mammary adipose tissue and from abdominal subcutaneous adipose tissue biopsies. In detail, mammary adipose tissue specimens were obtained from women (n = 12; age 25–63 years; BMI 24.2–29) undergoing surgical mammary reduction. All women were otherwise healthy and free from metabolic or endocrine diseases. Abdominal adipose tissue was obtained from obese (n = 10; age 45–65 years; BMI 30–35) and lean women (n = 10; age 32–60 years; BMI 20–25) by abdominal biopsy in the periumbilical region under local anaesthesia (2% lidocaine). Informed consent was obtained from every study participant before the surgical procedure. This procedure was approved by the ethical committee of the University of Naples. Adipose tissue was digested with collagenase and then adipose-derived SVF cells were isolated and differentiated as previously reported [23]. Conditioned media were obtained by incubating the cells for 8 h with serum-free DMEM containing 0.25% BSA after two washes with PBS. After the incubation, medium was collected and centrifuged at 14,000 g to remove cellular debris and placed onto recipient cells for different times, as indicated, or analysed for cytokines and growth factor content, as described below. For co-cultures, 8 × 104 MCF-7 cells were seeded in the upper chamber of a transwell culture system (4 μm pore size, Costar plates, Corning Life Sciences, Lowell, MA, USA) in a complete medium. The following day, the cells were incubated in serum-free DMEM 0.25% BSA with or without adipocytes in the lower chamber. Sodium oleate and sodium palmitate were dissolved in NaOH at 100 mmol/l final concentration and conjugated with 10% fatty acid-free BSA (as a physiological carrier) at a molar ratio of 3:1 [24].
Cell proliferation and viability
MCF-7 (1 × 105 cells/well) and MDA-MB-231 (1 × 105 cells/well) cells were seeded in six-well culture plates in a complete medium. The following day, the cells were starved in serum-free DMEM 0.25% BSA for 16 h and incubated with conditioned media obtained as described above for different times. Cell count was performed either by Bürker chamber and with the TC10 Automated Cell Counter (Bio-Rad) according to the manufacturer’s protocol. Sulforhodamine assay or crystal violet staining were used for cell viability determination [25, 26].
Flow cytometry
MCF-7 cells were kept in the presence or in the absence of serum or incubated with conditioned media, as indicated. Cell cycle phases were then assayed by cytometric analysis as previously described [27].
Cytokine and growth factor assay
3T3-L1 and human pre-adipocyte and adipocyte conditioned media were screened for the concentration of IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17, eotaxin, granulocyte-colony stimulating factor (G-CSF), granulocyte–macrophage colony stimulating factor (GM-CSF), IFN-γ, keratinocyte-derived chemokine (KC)/IL-8, monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1-alpha/beta (MIP-1α, MIP-1β), RANTES, TNF-α, platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and IGF-1, using the Bio-Plex multiplex Mouse and Human Cytokine and Growth factor kits (Bio-Rad) according to the manufacturer’s protocol.
Real-time RT-PCR analysis
Total RNA was isolated from 3T3-L1 and human pre-adipocytes and adipocytes by using the Rneasy Kit (Qiagen Sciences, Germantown, MD, USA) according to the manufacturer’s instruction. For real-time RT-PCR analysis, 1 μg cell RNA was reverse transcribed using SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). PCRs were analysed using SYBR Green mix (Invitrogen). Reactions were performed using Platinum SYBR Green Quantitative PCR Super-UDG using an iCycler IQ multicolor Real-Time PCR Detection System (Bio-Rad). All reactions were performed in triplicate and β-actin was used as an internal standard. Primer sequences used are described in electronic supplementary material (ESM) Table 1.
Immunoblot procedure
Total cell lysates were obtained and separated by SDS-PAGE as previously described [27].
Phosphoprotein assay
Protein lysates were prepared by using Cell lysis kit (Bio-Rad) and the presence of phosphorylated Akt/PKB, ERK, STAT3 and IGF-1R was detected by Bio-Plex Phospho- Ser473 Akt1/PKB, Phospho-Thr202/Tyr204 ERK, Phospho-Tyr705 STAT3 and Phospho-Tyr1131 IGF-1R Assay kits and the Phosphoprotein Testing Reagent kit (Bio-Rad) according to the manufacturer’s protocol. The total proteins for Akt1/PKB, ERK and STAT3 were tested using the Bio-Plex 3-plex assay kit (Bio-Rad).
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Statistical analysis
Data were analysed with Statview software (Abacus concepts) by one-factor analysis of variance. A p-value of less than 0.05 was considered statistically significant.
Results
Adipocytes promote breast cancer cell survival
We first analysed the impact of adipocyte-secreted factors on breast cancer cell growth, in comparison with factors released by undifferentiated fibroblasts. Conditioned medium (CM) from 3T3-L1 adipocytes induced a time-dependent increase of cell growth, which was significantly more effective than that induced by CM from 3T3-L1 fibroblasts (Fig. 1a) and similar to that observed for cells incubated with 10% FBS-medium (Fig. 1a). Consistent results were obtained with the ERα-negative MDA-MB-231 cells (Fig. 1b), suggesting that the effect was independent of oestrogen release. To investigate whether human adipocytes were as effective as murine cell lines in promoting breast cancer cell growth, we obtained adipose tissue from breast surgery. As shown in Fig. 1c, CM from adipocytes, either differentiated from SVF or directly isolated from mammary specimens, induced growth of MCF-7 cells, by 1.5 fold and twofold, respectively, while CM from undifferentiated SVF cells had no significant effect. Similar data were also obtained with MDA-MB-231 cells (data not shown). Consistently, as assessed by sulforhodamine assay, cell viability was increased by 3T3-L1 and human mammary adipocyte CM by 1.6 and 2.6 fold, respectively compared with their undifferentiated precursors (Fig. 1d).
Fig. 1
Effect of adipocyte-released factors on breast cancer cell growth. (a) Undifferentiated mouse 3T3-L1 cells or mature 3T3-L1 adipocytes were incubated with serum-free DMEM for 8 h. Media were collected from undifferentiated 3T3-L1 cells and from mature 3T3-L1 adipocytes (respectively, 3T3-L1 CM [grey diamonds] and Adipo 3T3-L1 CM [black squares]) and added to serum-starved MCF-7 breast cancer cells for 6, 12, 24 and 48 h. As a control, MCF-7 cells were also incubated with DMEM without serum supplementation (DMEM-BSA [white triangles]) or with 10% FBS (DMEM 10% FBS [white circles]). The cells were then counted as described in the Methods and the results reported as fold increase over basal (cell count in DMEM-BSA). (b) 3T3-L1 CM and Adipo 3T3-L1 CM were added to serum-starved MDA-MB-231 breast cancer cells for 24 h. As a control, MDA-MB-231 cells were also incubated with DMEM-BSA or with DMEM 10% FBS. Cells were then counted as described in the Methods and the results reported as fold increase over basal (cell count in DMEM-BSA). (c) Adipose-derived SVF, differentiated adipocytes (hAdipo) or mature adipocytes (Isol. Adipo) isolated from breast surgery (n = 12) were incubated with serum-free medium for 8 h. Media were collected (CM) and added to serum-starved MCF-7 cells for 24 h. As a control, MCF-7 cells were also incubated with medium without serum supplementation (basal medium). Cells were then counted and the results reported as fold increase over basal (cell count in basal medium). (d) 3T3-L1 CM, Adipo 3T3-L1 CM, SVF CM and hAdipo CM were added to serum-starved MCF-7 for 48 h. Cell viability was then assessed by sulforhodamine assay as described in the Methods and the results reported as percentage of viable cells compared with cells in DMEM 10% FBS, considered as 100% viable cells. (e) MCF-7 cells were seeded in the upper chamber of a transwell culture system with or without 3T3-L1, Adipo 3T3-L1, SVF or hAdipo in the lower chamber and cell viability was determined by crystal violet staining as described in the Methods. The results are reported as percentage of stained cells. *p < 0.05, **p < 0.01, ***p < 0.001 compared with basal; †p < 0.05; ††p < 0.01 compared with 3T3-L1 or SVF CM. (f) MCF-7 cells were starved in serum-free DMEM for 18 h and incubated with 3T3-L1 CM, Adipo 3T3-L1 CM, SVF CM and hAdipo CM for 24 h. Cells were stained with propidium iodide and the ratio of cells in sub-G1 phase was determined by cytometric analysis, as described in the Methods. *p < 0.05, comparing indicated data For all the panels in the figure, data in the graphs represent the mean ± SD of at least four independent triplicate experiments
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Again, when co-cultured with 3T3-L1 and human adipocytes, MCF-7 displayed higher viability, comparable with that observed for cells cultured in DMEM–10% FBS. A much lower effect was observed when MCF-7 cells were co-cultured with either 3T3-L1 fibroblasts or SVF undifferentiated cells (Fig. 1e).
To further investigate whether growth-promoting action was dependent on oestrogen release by mature adipocytes, MCF-7 cells were incubated with mammary adipocyte CM in the presence of 1 μmol/l Tamoxifen, a selective oestrogen receptor antagonist (ESM Fig. 1). No significant effect was elicited by tamoxifen on the adipocyte growth-promoting action. Also, exposure of the cells to anti-leptin antibodies did not change the effect of adipocyte CM on cancer cell growth (ESM Fig. 1).
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Moreover, incubation of MCF-7 cells with CM from 3T3-L1 fibroblasts only slightly decreased the number of cell deaths induced by growth factor deprivation, reducing the number of cells in sub-G1 phase by 25% (Fig. 1f). Interestingly, cell death was substantially rescued by incubation with CM from 3T3-L1 adipocytes, at levels similar to those achieved with 10%FBS medium (Fig. 1f). Again, the number of cell deaths was decreased following incubation with CM from mammary adipocytes, and from undifferentiated SVF cells (Fig. 1f), although the effect of the latter CM was lower.
Adipocyte factors induce MAPK, PI3K and JAK/STAT activation in MCF-7 cells
To gain further insight into the molecular mechanism by which adipocytes promote breast cancer cell growth, we tested the ability of adipocyte conditioned media to induce mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K) and JAK/STAT activation. To this aim, MCF-7 cells were treated with CM from 3T3-L1 or from mammary adipocytes for 12 h (ESM Fig. 2). Western blot and phosphoprotein assay revealed that CM induced the phosphorylation of ERK, Akt and STAT3 (ESM Fig. 2).
Release of cytokines/chemokines and growth factors by 3T3-L1 and human mammary adipocytes
Based on the observed induction of signalling pathways, we have screened for the ability of 3T3-L1 and mammary adipocytes to release cytokines/chemokines and growth factors. Both 3T3-L1 fibroblasts and undifferentiated SVF cells released detectable amounts of IL-4, IL-6, IL-10, IFNγ, KC/IL-8, MIP-1β, RANTES and TNF-α; MIP-1α was exclusively detected in the medium of 3T3-L1 pre-adipocytes and not of human SVF cells. However, the levels of KC/IL-8 and RANTES were higher both in 3T3-L1 and human adipocytes, compared with their respective precursors. Moreover, increased levels of IL-6 and MIP-1β were detected in the CM of human adipocytes while not in the CM of 3T3-L1 adipocytes.
We also screened for growth factor release and found that VEGF, bFGF, PDGF and IGF-1 were detectable in both pre-adipocytes and adipocytes. However, while there was no significant difference in the content of PDGF and bFGF, the amount of VEGF was significantly reduced in the CM of both 3T3-L1 and human mammary adipocytes. At variance, IGF-1 was about fivefold higher in the CM of differentiated 3T3-L1 and mammary adipocytes (Table 1).
Table 1
Adipocyte-released cytokines and growth factors
Cytokine/growth factor
3T3-L1 CM (pg/ml)
Adipo 3T3-L1 CM (pg/ml)
SVF CM (pg/ml)
hAdipo CM (pg/ml)
IL-2
ND
ND
ND
ND
IL-4
0.7 ± 0.008
0.10 ± 0.02
1.12 ± 0.66
1.36 ± 0.66
IL-6
23.6 ± 1.13
22.37 ± 3.47
2,705.58 ± 541
5,005.06 ± 1,200†
IL-10
3.70 ± 0.86
15 ± 2.6*
10.47 ± 2.24
6.53 ± 1.66†††
GM-CSF
ND
ND
ND
ND
IFN-γ
3.73 ± 0.38
5.59 ± 0.80
46.70 ± 9.2
41.64 ± 19.8
KC/IL-8
989.14 ± 100.62
10,343.72 ± 1,551.53*
2,975.59 ± 300
15,647.85 ± 190.2††
MIP-1α
226.31 ± 18,27
280 ± 22.32
ND
ND
MIP-1β
10.33 ± 1.53
11.4 ± 2
3.37 ± 1.02
25.88 ± 9.01††
RANTES
1,960 ± 235.2
4,770 ± 524.7*
12.27 ± 1.5
52.99 ± 10††
TNF-α
143.54 ± 18.66
162.88 ± 25.92
1.54 ± 0.80
2.17 ± 1.10
PDGF
ND
ND
1.20 ± 0.90
2.65 ± 1.32
bFGF
200 ± 18
660.70 ± 2.67*
19.37 ± 3.87
25.58 ± 5.37
VEGF
11,479.20 ± 1,262.71
4,682.46 ± 607.71*
526.53 ± 105.30
295.58 ± 65.02††
IGF-1
79.37 ± 0.88
334.37 ± 40.41*
28.85 ± 0.50
136.66 ± 5.7††
Data represent the mean ± SD of at least four independent triplicate experiments. Supernatants from mouse 3T3-L1 pre-adipocytes (3T3-L1 CM), 3T3-L1 adipocytes (Adipo 3T3-L1 CM), human SVF (SVF CM) and differentiated adipocytes (hAdipo CM were collected for 8 h and tested by using the Bio-Plex multiplex cytokine assay kit and the Bio-Plex multiplex growth factor assay kit as described in the Methods
*p < 0.05 for 3T3-L1 adipocytes vs 3T3-L1 CM; †p < 0.05, ††p < 0.01, †††p < 0.001 for human adipocytes vs SVF CM
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Similar to protein levels in the conditioned media, Kc (also known as Cxcl1)/IL-8, RANTES and IGF-1 mRNA levels were higher by 5, 6 and 6.5 fold, and 4, 4.5 and 7.5 fold, respectively, in differentiated 3T3-L1 cells and in human adipocytes, compared with their undifferentiated counterparts (Fig. 2).
Fig. 2
Kc/IL-8, RANTES and IGF-1 expression in differentiated adipocytes vs undifferentiated precursors. Undifferentiated 3T3-L1 and SVF cells or mature 3T3-L1 and human adipocytes were tested for the levels of mouse Kc, Rantes and Igf-1 mRNA, or of human IL-8, RANTES and IGF-1 mRNA by real-time RT-PCR analysis, as described in the Methods. Dark grey columns represent 3T3-L1 adipocytes; light grey columns represent human adipocytes. Bars represent the mean ± SD of four independent experiments and show the mRNA levels in these cells relative to those in 3T3-L1/human adipocyte precursor cells. *p < 0.05, **p < 0.01, ***p < 0.001, in comparison with the respective measurement in precursor cells
×
Glucose and fatty acids modify adipocyte growth-promoting action
Next, 3T3-L1 adipocytes were cultured for 24 h either in 5.5 mmol/l glucose (LG, low glucose), a concentration corresponding to normal fasting glucose levels in humans, or in 25 mmol/l glucose (HG, high glucose), corresponding to the regular culture condition for this cell line, but resembling hyperglycaemia in humans. Moreover, we added to culture media either 0.5 μmol/l oleate or 10 μmol/l palmitate, two major fatty acids whose plasma levels are increased in dysmetabolic conditions. Media were changed and cells were allowed to secrete factors into freshly added serum-free medium. After 8 h, media were collected and tested for the content of cytokines/chemokines and growth factors. No significant change was detected for IL-4, IL-6, IL-10, IFN-γ, MIP-1α, MIP-1β, TNF-α, PDGF, bFGF and VEGF (data not shown). However, 3T3-L1 adipocytes cultured in HG medium released significantly higher levels of KC (Fig. 3a), RANTES (Fig. 3b) and IGF-1 (Fig. 3c), compared with those cultured in LG medium. Very similar results were obtained for IL-8 (Fig. 3d), RANTES (Fig. 3e) and IGF-1 (Fig. 3f) in differentiated mammary adipocytes. Interestingly, culturing 3T3-L1 and human adipocytes with either oleate or palmitate in LG medium was accompanied by a significant increase of KC/IL-8, RANTES and IGF-1 (Fig. 3a–f).
Fig. 3
Effect of glucose and fatty acids on adipocyte-released cytokines and growth factors. Mouse 3T3-L1 adipocytes (a–c) and human adipocytes (d–f) were pre-incubated with high glucose DMEM (25 mmol/l glucose, HG) or with low glucose DMEM (5.5 mmol/l glucose, LG) for 24 h in the presence or absence of 10 μmol/l palmitate (PAL) or 0.5 μmol/l oleate (OLE) for 18 h. They were then further incubated with serum-free DMEM for 8 h. Media were collected (CM) and tested by using the Bio-Plex multiplex cytokine assay kit and the Bio-Plex multiplex growth factor assay kit. Values ± SD for mouse KC (a), RANTES (b) and IGF-1 (c), and for human IL-8 (d), RANTES (e) and IGF-1 (f) are reported in the bar graph *p < 0.05, comparing indicated data and represent the mean ± SD of at least four independent triplicate experiments
×
We next addressed whether regulation of those adipocyte-produced factors occurred at the transcriptional level. Kc and Igf-1 mRNA levels were about twofold higher in 3T3-L1 adipocytes cultured in HG medium compared with LG medium (Fig. 4). Similarly, in differentiated human adipocytes, both IL-8 and IGF-1 levels were higher in HG medium than in LG medium. At variance, RANTES (also known as CCL5) mRNA levels were comparable in HG and LG media, both in 3T3-L1 and human adipocytes.
Fig. 4
Glucose and fatty acids control Kc/IL-8, RANTES and IGF-1 expression. Mouse 3T3-L1 adipocytes (a–c) and human adipocytes (hAdipo) (d–f) were pre-incubated with either HG DMEM or with LG DMEM for 24 h in the presence or absence of 10 μmol/l palmitate (PAL) or 0.5 μmol/l oleate (OLE) for 18 h. Following this, mRNA levels of mouse Kc (a), Rantes (b) and Igf-1 (c), and of human IL-8 (d), RANTES (e) and IGF-1 (f) were determined by real-time RT-PCR analysis. Data were normalized on β-actin as internal standard. Bars represent the mean ± SD of four independent experiments and show the mRNA levels in these cells relative to those in 3T3-L1/hAdipo HG cells, reported as 1 in the first column of each bar graph. *p < 0.05, **p < 0.01, comparing indicated data. Rel. expr., relative expression
×
However, when oleate or palmitate were added to LG medium, significant increases of RANTES and IGF-1 (also known as IGF1) mRNA levels, while not of Kc/IL-8, were observed both in 3T3-L1 and human adipocytes.
Thus, these data suggest that glucose may upregulate Kc/IL-8 and IGF-1, while fatty acids may induce RANTES and IGF-1 at mRNA levels.
Therefore, we tested whether glucose and fatty acids may change the growth-promoting action of murine and human adipocytes. To this end, MCF-7 cells were incubated for 24 h in the presence of either LG or HG conditioned media from 3T3-L1 and human mammary adipocytes. Pre-incubation of adipocytes (both 3T3-L1 and human) with HG medium enhanced, by about twofold, their ability to induce MCF-7 cell growth, compared with LG medium (Fig. 5). Moreover, the presence of palmitate or oleate in the LG medium (from both 3T3-L1 and human adipocytes) significantly increased breast cancer cell growth (Fig. 5).
Fig. 5
Effect of glucose and fatty acids on adipocyte growth-promoting action. Mouse 3T3-L1 adipocytes (a) and human adipocytes (b) were pre-incubated with either HG DMEM or with LG DMEM for 24 h in the presence or absence of 10 μmol/l palmitate or 0.5 μmol/l oleate for 18 h. They were then further incubated with serum-free DMEM for 8 h. Media were collected (CM) and added to serum-starved MCF-7 cells for 24 h. Cells were then counted and the results reported as fold increase over basal (cell count in DMEM-BSA). *p < 0.05, **p < 0.01, comparing indicated data. Data in the bar graphs represent the mean ± SD of at least four independent triplicate experiments
×
Induction of breast cancer cell growth by adipocytes from lean and obese individuals
SVF cells were isolated from abdominal liposuctions carried out in lean (n = 10) and obese (n = 10) individuals. These cells were differentiated into mature adipocytes and conditioned media were collected as previously described (see Methods). As for mammary-derived specimens (see Fig. 1c), abdominal subcutaneous differentiated adipocytes induced MCF-7 cell growth to a larger extent, compared with undifferentiated SVF cells (Fig. 6) from both lean and obese individuals. However, CM from both undifferentiated SVF cells and differentiated adipocytes obtained from obese individuals were significantly more effective than those obtained from lean individuals in inducing MCF-7 cell growth.
Fig. 6
Cancer cell growth induced by adipocytes from lean and obese individuals. SVF cells and human adipocytes isolated from obese (BMI 30–35) and lean women (BMI 20–25) were incubated with serum-free medium for 8 h. Media were collected (CM) and added to serum-starved MCF-7 cells for 24 h. Then, cells were counted and the results reported as fold increase over basal (cell count in medium without serum supplementation). Dark grey columns represent SVF CM; light grey columns represent human adipocyte (hAdipo) CM. *p < 0.05, comparing indicated data. Data in the bar graphs represent the mean ± SD of at least four independent triplicate experiments
×
Again, as for mammary adipocytes, the release of IL-8, RANTES and IGF-1 was more abundant in mature subcutaneous adipocytes, compared with their undifferentiated counterparts (Table 2), both for lean and obese individuals. No difference in IL-4, IL-8, IL-10, IFN-γ, MIP-1α, MIP-1β, RANTES, TNF-α, PDGF, bFGF and VEGF content was found in the media of SVF cells and mature adipocytes from lean and obese individuals. Nevertheless, IL-6 and IGF-1 were significantly more abundant in CM of obese compared with lean adipocytes (Table 2). Moreover, only IGF-1 levels were higher in SVF cells from obese individuals than in those from lean individuals.
Table 2
Cytokines and growth factors released by adipocytes from lean and obese individuals
Cytokine/growth factor
BMI 21–25
BMI 30–35
SVF CM (pg/ml)
hAdipo CM (pg/ml)
SVF CM (pg/ml)
hAdipo CM (pg/ml)
IL-2
ND
ND
ND
ND
IL-4
1.09 ± 0.60
1.49 ± 0.53
0.79 ± 0.06
0.96 ± 0.006
IL-6
2,446.87 ± 489.37
6,420.31 ± 1,348.26*
2,180.64 ± 436.12
12,367.95 ± 2,597.26*††
IL-10
11.40 ± 2.66
6.75 ± 1.48*
9.61 ± 2.32
5.90 ± 1.99*
GM-CSF
ND
ND
ND
ND
IFN-γ
45.82 ± 9.62
46.7 ± 10.27
38.86 ± 12.72
41.64 ± 18.80
IL-8
2,556.45 ± 350.45
25,019.32 ± 1,264.48**
1,229.32 ± 380.12
22,271.66 ± 5,308.75**
MIP-1α
58.82 ± 7.98
136.23 ± 9.62
54.14 ± 4.80
52.95 ± 7.83
MIP-1β
10.33 ± 3.46
27.96 ± 3.27*
3.52 ± 0.58
34.62 ± 8.44*
RANTES
17.98 ± 3.40
73.65 ± 9.68*
12.45 ± 2.27
45.26 ± 11.35**
TNF-α
204.08 ± 15.35
291.65 ± 35.82
173.49 ± 18.52
200.04 ± 21.71
PDGF
2.48 ± 1.35
3.04 ± 0.88
2.80 ± 0.52
3.16 ± 0.71
bFGF
28.63 ± 11.90
36.88 ± 12.66
14.68 ± 3.74
24.55 ± 6.27
VEGF
620.53 ± 165.61
178.54 ± 85.01**
483.06 ± 29.48
322.20 ± 71.55
IGF-1
115 ± 54.46
666 ± 40.42*
193.33 ± 64.29†
1,073.33 ± 22.30**†
SVF cells and differentiated SVF (hAdipo) isolated from obese (BMI 30–35; n = 10) and lean women (BMI 20–25; n = 10) were incubated with serum-free medium for 8 h. Media were collected (CM) and analysed by using the Bio-Plex multiplex human cytokine assay kit and the Bio-Plex multiplex human growth factor assay kit. Determinations were done in triplicate
*p < 0.05, **p < 0.01 for adipocytes vs SVF CM within the same group; †p < 0.05; ††p < 0.01 for SVF or hAdipo CM of one group vs the other
ND, no data
Effect of IGF-1 pathway inhibition on cell growth
To address the biological relevance of IGF-1 as an adipocyte-derived growth-promoting factor, MCF-7 cells were treated with HG CM in the presence of 10 μmol/l AG1024, a specific inhibitor of IGF-1R kinase activity.
AG1024 almost completely prevented the effect of 3T3-L1 CM on MCF-7 cell growth (Fig. 7a). Very similar results were obtained for the prevention of the effect induced by mammary (Fig. 7b) as well as subcutaneous abdominal adipocytes, both from lean and from obese individuals (Fig. 7c). Indeed, the induction of MCF-7 cell growth by lean and obese adipocytes was reduced at comparable levels, following treatment with AG1024 (Fig. 7c). Moreover, the increased growth induced by SVF cells from obese individuals was fully rescued by AG1024 (Fig. 7c) and returned to levels similar to those achieved by SVF cells from lean individuals. Similarly, conditioned media from obese adipocytes induced IGF-1R phosphorylation twofold more effectively than those from lean individuals (Fig. 7d). Also, only SVF cells from obese, and not from lean, individuals were able to increase IGF-1R phosphorylation. Finally, these effects were completely blocked by AG1024, indicating a major involvement of IGF-1 (Fig. 7d).
Fig. 7
Effect of IGF-1 pathway inhibition on cell growth. Conditioned media collected from mouse 3T3-L1 adipocytes (a) and mammary adipocytes (b) and subcutaneous abdominal SVF and adipocytes isolated from obese (BMI 30–35) and lean women (BMI 20–25) (c) were added to serum-starved MCF-7 cells for 24 h in the presence or absence of 10 μmol/l AG1024. Cells were then counted and the results reported as fold increase over basal (cell count in serum-free medium). White columns, SVF CM; light grey columns, SVF CM + Ag1024; dark grey columns, human adipocyte (hAdipo) CM; black columns, hAdipo CM + Ag1024. (d) MCF-7 cells were exposed to conditioned media collected from subcutaneous abdominal SVF and adipocytes isolated from obese (BMI 30–35) and lean women (BMI 20–25) for 24 h and proteins extracted were tested for the presence of phosphorylated IGF-1R by using the Bio-Plex phosphoprotein assay kit as described in Methods. White columns, SVF CM; light grey columns, SVF CM + Ag1024; dark grey columns, hAdipo CM; black columns, hAdipo CM + Ag1024. *p < 0.05, **p < 0.01, comparing indicated data. Data in the bar graphs represent the mean ± SD of at least four independent triplicate experiments
×
Discussion
Convincing evidence links obesity and diabetes to the risk of several forms of cancer [4, 11, 14]. We now describe that differentiated adipocytes, both of mouse and of human origin, have a significant impact in enhancing breast cancer cell survival and proliferation. Consistent with previous reports [16], the effect of adipocytes is larger than that of their precursor cells. Interestingly, however, their growth-promoting action is paralleled by a specific secretory pattern of inflammatory cytokines, chemokines and growth factors, which is also affected by culture conditions. In particular, both glucose and fatty acids enhanced the ability of adipocytes to induce the growth of breast cancer cells. When cultured in 25 mmol/l glucose (HG), a condition mimicking hyperglycaemia, both 3T3-L1 and human breast mammary adipocytes were more effective in inducing growth of MCF-7 and MDA-MB-231 cells, compared with when they were cultured in 5.5 mmol/l glucose (LG), which represents normal glycaemia in humans.
Incubation of adipocytes with fatty acids also led to increased growth-promoting action, suggesting that metabolic perturbations may change their secretory pattern. This effect was observed with palmitate or oleate, either individually or in combination (data not shown). Higher concentrations of fatty acids (up to 100 μmol/l palmitate and 10 μmol/l oleate) had a slightly higher effect on both cancer cell growth and secretory profile (data not shown). Moreover, it is known that high concentrations of fatty acids impair insulin sensitivity [28] and this may further affect adipocyte functions.
It has been reported that adipocyte-derived molecules can affect cancer cell growth. For instance, leptin has been shown to induce proliferation of breast cancer cells [29]. However, we found that glucose and fatty acids had no effect on leptin release by mammary adipocytes (data not shown). Nevertheless, treatment of MCF-7 cells with anti-leptin blocking antibodies did not rescue the effect of HG adipocyte CM on cell growth (ESM Fig. 1). Adipocyte-derived oestrogens have also been related to breast cancer development and progression [30]. It seems unlikely that oestrogens could mediate the effect of glucose and fatty acids, however, since adipocyte CM elicited a similar effect in the oestrogen-independent MDA-MB-231 cells and it was unchanged following tamoxifen treatment of MCF-7 cells. We therefore tested whether glucose and fatty acids could modify the release of cytokines, chemokines and growth factors by adipocytes. The secretory patterns obtained by mouse 3T3-L1 and human mammary adipocytes were somewhat different. For example, MIP-1α and TNF-α were more abundant in 3T3-L1 cells, while IL-6 and IFN-γ were more abundant in human cells. However, IL-8 and RANTES were released to a larger extent by both differentiated 3T3-L1 cells and human adipocytes, compared with their precursors, indicating that common changes were present in the differentiation process. The increased release of IL-8 and RANTES was paralleled by a similarly sized increase in their mRNA levels.
Interestingly, both glucose and fatty acids increased the release of IL-8 and RANTES, suggesting that the metabolic environment could further modify adipocyte releasing properties. Similar results were also found for IGF-1, which was more abundant in the medium of mature adipocytes and upregulated by glucose and fatty acids. bFGF also tended to be detected in higher amounts in the mature adipocytes, while VEGF levels were more elevated in the undifferentiated precursors. However, neither bFGF nor VEGF were significantly regulated by nutrients.
We have obtained evidence that glucose and fatty acids may regulate IL-8, RANTES and IGF-1 at different levels. Culturing adipocytes in HG is accompanied by a significant upregulation of IL-8 and IGF-1, while not of RANTES mRNA, compared with low glucose. At variance, both oleate and palmitate enhance IGF-1 and RANTES, while not IL-8 mRNA levels. Altogether, these data raise the possibility that, beside regulating gene expression, nutrients may control adipocyte secretion process.
Thus, these findings are consistent with the hypothesis that adipocytes, in an appropriate metabolic environment, may contribute to tumour growth and progression by releasing factors that may function to promote cancer cell proliferation. In particular, RANTES, IL-8 and IGF-1 represent good candidates since: (1) they are produced in the adipocytes and released in the culture medium; (2) their production/release is induced by nutritional factors, such as glucose and fatty acids; and (3) they have been implicated in the control of cancer cell phenotypes. For instance, RANTES and IL-8 are elevated in plasma of obese individuals [31‐33] and have been largely implicated in obesity-related health complications [34‐36] as well as in the mechanisms of cancer progression [37‐39].
IGF-1 also plays a pivotal role in the progression of many forms of cancer, including breast cancer [40‐42]. Indeed IGF-1 is involved in the development of normal mammary gland and in the onset and/or progression of mammary tumours [42], as well as in the development of drug resistance [43].
It was recently reported that, in pancreatic cancer, autocrine production of IGF-1 by cancer cells enhances the production of RANTES by stromal cells, thereby providing a paracrine loop to facilitate tumour progression [44]. Moreover, expression profile studies indicate that IL-8 and IGF-1 are produced at higher levels in tissues from patients with high-grade breast cancer than in tissues from patients with low-grade breast cancer [45].
We have also found that adipocytes obtained from subcutaneous fat specimens from obese individuals were capable of producing larger amounts of IGF-1 compared with lean controls. This is consistent with higher circulating IGF-1 levels often found in obesity [46]. Surprisingly, the difference was also significant when IGF-1 was measured in the conditioned media of undifferentiated SVF cells from obese individuals. Thus, IGF-1 upregulation appears to occur early during adipocyte differentiation, is enhanced in obesity and is maintained in cultured mesenchymal stem cells. This could possibly be due to epigenetic changes in IGF-1 gene expression regulation [47]. Interestingly, however, upregulation was only observed for IGF-1 and not for other chemokines and growth factors, both in undifferentiated SVF and in mature adipocytes.
IGF-1 in the adipose tissue may act either as an autocrine factor [48], regulating adipose tissue development and homoeostasis, or in a paracrine manner, regulating survival and function of the surrounding cells, including cancer cells. Inhibition of IGF-1 function, indeed, almost completely abolished the effect of adipocyte-released factors, thereby indicating that IGF-1 is a pivotal factor in adipocyte regulation of cancer cell growth. However, these results are limited to breast cancer models, in which the importance of the local environment, including adipocyte function, has been largely documented. Further studies are needed to assess the relevance of adipose-related factors in the control of other cancer types. Moreover, the communications between cancer cells and adipocytes may be bidirectional. Release of TNF-α from cancer cells may affect adipocyte differentiation and function, possibly increasing NEFA release, to provide additional feed for the cancer cells [49, 50].
Thus, we have described that IGF-1 release by adipocytes is enhanced in obesity and is regulated by metabolic perturbations. Other factors, such as RANTES and IL-8, which are not primarily upregulated in obese individuals, are also enhanced by glucose and fatty acids and may as well contribute to adipocyte control of breast cancer cell growth.
Acknowledgements
The authors are grateful to S. Libertini (University of Naples, Naples, Italy) and C. Passaro (University of Naples, Naples, Italy) for technical help with cytofluorimetric assays and to G. Perruolo (CNR, Naples, Italy) and G. Portella (University of Naples, Naples, Italy) for helpful discussion and advice.
Funding
This study was supported in part by the European Community’s FP6 PREPOBEDIA (201681), the European Foundation for the Study of Diabetes (EFSD), the Associazione Italiana per la Ricerca sul Cancro (AIRC) and by the Ministero dell’Università e della Ricerca Scientifica (grants PRIN and FIRB-MERIT).
Duality of interest
The authors declare that there is no duality of interest associated with this manuscript.
Contribution statement
VD’E was the main contributor in terms of conception, design, acquisition and interpretation of data and in drafting the article. FP, AH, DL, DT, GM, LC and CM mainly contributed in conceptual design and acquisition of data. FB and US mainly contributed in terms of conceptual design, analysis, interpretation and discussion of the results. PF mainly contributed in terms of conceptual design, interpretation and discussion of the results and supervision of the overall work. All the authors critically revised the article and approved the final version.
Open Access
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
Open AccessThis is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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