nach oben

Journal of Cardiovascular Translational Research

Erschienen in:

19.09.2018 | Original Article

Aerobic Exercise Prevents Insulin Resistance Through the Regulation of miR-492/Resistin Axis in Aortic Endothelium

verfasst von: Ying Cai, Kang-Ling Xie, Fan Zheng, Sui-Xin Liu

Erschienen in: Journal of Cardiovascular Translational Research | Ausgabe 6/2018

Einloggen, um Zugang zu erhalten

Abstract

Previously, we found that miR-492 delayed the progression of atherosclerosis (AS) by acting as an up-stream regulator of resistin. Therefore, we hypothesized that the anti-atherogenic effects of exercise are related to miR-492-mediated downregulation of resistin and repair of endothelial injury. In this study, we investigated the effects of the miR-492/resistin axis on improving endothelial injury in ApoE−/− mice (ApoE-deficient/knockout in C57BL/6 mice) through swimming exercises. Our results showed that the severity of AS and insulin resistance (IR) in these mice were significantly reduced by swimming exercises. In addition, miR-492 expression in the aortic endothelium of ApoE−/− mice was decreased, in addition to increased levels of resistin. Interestingly, swimming exercises increased miR-492 expression while decreasing that of resistin. Taken together, swimming exercises delayed the progression of AS, possibly by upregulating miR-492 and downregulating resistin in aortic endothelium. Therefore, exercises modulated glucose and lipid metabolism, alleviated endothelial IR, and repaired endothelial injury.

Tian, F., Yu, B. L., & Hu, J. R. (2014). mTOR mediates the cross-talk of macrophage polarization and autophagy in atherosclerosis. International Journal of Cardiology, 177(1), 144–145. https://doi.org/10.1016/j.ijcard.2014.09.035.CrossRefPubMed

Sun, C. H., Cai, Y., Zhang, W. L., Xie, K. L., Liu, Y., & Liu, S. X. (2013). The effects of swimming training on expression of PPAR-γ,CPT-1 and MCAD in ApoE knock-out mice. Chinese Journal of Rehabilitation Medicine, 28(2), 134–138.

Li, J. (2011). The effects of swimming training on myocardial PPAR-γ expression and glucose and lipid metabolism in mice with insulin resistance [D]. Changsha: Central South University.

Liu, H., Jiang, D., Zhang, S., & Ou, B. (2010). Aspirin inhibits fractalkine expression in atherosclerotic plaques and reduces atherosclerosis in ApoE gene knockout mice. Cardiovascular Drugs and Therapy, 24(1), 17–24. https://doi.org/10.1007/s10557-009-6210-7.CrossRefPubMed

Muniyappa, R., Montagnani, M., Koh, K. K., & Quon, M. J. (2007). Cardiovascular actions of insulin. Endocrine Reviews, 28(5), 463–491. https://doi.org/10.1210/er.2007-0006.CrossRefPubMed

Muniyappa, R., & Sowers, J. R. (2013). Role of insulin resistance in endothelial dysfunction. Reviews in Endocrine & Metabolic Disorders, 14(1), 5–12. https://doi.org/10.1007/s11154-012-9229-1.CrossRef

Zhang, Y., Li, L., You, J., Cao, J., & Fu, X. (2013). Effect of 7-difluoromethyl-5, 4′-dimethoxygenistein on aorta atherosclerosis in hyperlipidemia ApoE(-/-) mice induced by a cholesterol-rich diet. Drug Design, Development and Therapy, 7, 233–242. https://doi.org/10.2147/DDDT.S37512.CrossRefPubMedPubMedCentral

Melo, S. F., Fernandes, T., Barauna, V. G., Matos, K. C., Santos, A. A., Tucci, P. J., et al. (2014). Expression of MicroRNA-29 and collagen in cardiac muscle after swimming training in myocardial-infarcted rats. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 33(3), 657–669. https://doi.org/10.1159/000358642.CrossRef

Wu, X. D., Zeng, K., Liu, W. L., Gao, Y. G., Gong, C. S., Zhang, C. X., et al. (2014). Effect of aerobic exercise on miRNA-TLR4 signaling in atherosclerosis. International Journal of Sports Medicine, 35(4), 344–350. https://doi.org/10.1055/s-0033-1349075.CrossRefPubMed

10.

Cai, Y., Liu, S. X., Xie, K. L., Zhang, W. L., Dong, L., Liu, Y., et al. (2014). MicroRNA-492 reverses high glucose-induced insulin resistance in HUVEC cells through targeting resistin. Molecular and Cellular Biochemistry, 391(1–2), 117–125. https://doi.org/10.1007/s11010-014-1993-7.CrossRef

11.

Li, Q., Su, J., Jin, S. J., Wei, W., Cong, X. D., Li, X. X., et al. (2018). Argirein alleviates vascular endothelial insulin resistance through suppressing the activation of Nox4-dependent O2(-) production in diabetic rats. Free Radical Biology & Medicine, 121, 169–179. https://doi.org/10.1016/j.freeradbiomed.2018.04.573.CrossRef

12.

Nakashima, Y., Raines, E. W., Plump, A. S., Breslow, J. L., & Ross, R. (1998). Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse. Arteriosclerosis, Thrombosis, and Vascular Biology, 18(5), 842–851.CrossRef

13.

Nakashima, Y., Plump, A. S., Raines, E. W., Breslow, J. L., & Ross, R. (1994). ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arteriosclerosis and Thrombosis : A Journal of Vascular Biology, 14(1), 133–140.CrossRef

14.

Cheng, J., Song, J., He, X., Zhang, M., Hu, S., Zhang, S., et al. (2017). Erratum. Loss of Mbd2 protects mice against high-fat diet-induced obesity and insulin resistance by regulating the homeostasis of energy storage and expenditure. Diabetes 2016;65:3384-3395. Diabetes, 66(9), 2531. https://doi.org/10.2337/db17-er09b.CrossRefPubMedPubMedCentral

15.

Huang, X. S., Zhao, S. P., Hu, M., Bai, L., Zhang, Q., & Zhao, W. (2010). Decreased apolipoprotein A5 is implicated in insulin resistance-related hypertriglyceridemia in obesity. Atherosclerosis, 210(2), 563–568. https://doi.org/10.1016/j.atherosclerosis.2009.12.004.CrossRefPubMed

16.

Zhang, S. H., Reddick, R. L., Piedrahita, J. A., & Maeda, N. (1992). Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E. Science, 258(5081), 468–471.CrossRef

17.

Chen, Y., Yang, G. Y., & Ling, L. I. (2009). The changes of glucose-lipid metabolism in apoe~(-/-) mice with high-fat induced insulin resistance. Chinese Journal of Diabetes, 17(8), 590–593.

18.

Su, Z., Li, Y., James, J. C., Matsumoto, A. H., Helm, G. A., Lusis, A. J., et al. (2006). Genetic linkage of hyperglycemia, body weight and serum amyloid-P in an intercross between C57BL/6 and C3H apolipoprotein E-deficient mice. Human Molecular Genetics, 15(10), 1650–1658. https://doi.org/10.1093/hmg/ddl088.CrossRefPubMed

19.

Ruderman, N. B., & Haudenschild, C. (1984). Diabetes as an atherogenic factor. Progress in Cardiovascular Diseases, 26(5), 373–412.CrossRef

20.

von Eckardstein, A., Schulte, H., & Assmann, G. (2000). Risk for diabetes mellitus in middle-aged Caucasian male participants of the PROCAM study: Implications for the definition of impaired fasting glucose by the American Diabetes Association. Prospective Cardiovascular Munster. The Journal of Clinical Endocrinology and Metabolism, 85(9), 3101–3108. https://doi.org/10.1210/jcem.85.9.6773.CrossRef

21.

Kendall, D. M. (2005). The dyslipidemia of diabetes mellitus: Giving triglycerides and high-density lipoprotein cholesterol a higher priority? Endocrinology and Metabolism Clinics of North America, 34(1), 27–48. https://doi.org/10.1016/j.ecl.2004.11.004.CrossRefPubMed

22.

Tian, J., Pei, H., James, J. C., Li, Y., Matsumoto, A. H., Helm, G. A., et al. (2005). Circulating adhesion molecules in apoE-deficient mouse strains with different atherosclerosis susceptibility. Biochemical and Biophysical Research Communications, 329(3), 1102–1107. https://doi.org/10.1016/j.bbrc.2005.02.090.CrossRefPubMed

23.

Liu, Y., Wang, Q., Pan, Y. B., Gao, Z. J., Liu, Y. F., & Chen, S. H. (2008). Effects of over-expressing resistin on glucose and lipid metabolism in mice. Journal of Zhejiang University. Science. B, 9(1), 44–50. https://doi.org/10.1631/jzus.B071479.CrossRefPubMedPubMedCentral

24.

Moon, B., Kwan, J. J., Duddy, N., Sweeney, G., & Begum, N. (2003). Resistin inhibits glucose uptake in L6 cells independently of changes in insulin signaling and GLUT4 translocation. American Journal of Physiology. Endocrinology and Metabolism, 285(1), E106–E115. https://doi.org/10.1152/ajpendo.00457.2002.CrossRefPubMed

25.

Palanivel, R., & Sweeney, G. (2005). Regulation of fatty acid uptake and metabolism in L6 skeletal muscle cells by resistin. FEBS Letters, 579(22), 5049–5054. https://doi.org/10.1016/j.febslet.2005.08.011.CrossRefPubMed

26.

Sato, N., Kobayashi, K., Inoguchi, T., Sonoda, N., Imamura, M., Sekiguchi, N., et al. (2005). Adenovirus-mediated high expression of resistin causes dyslipidemia in mice. Endocrinology, 146(1), 273–279. https://doi.org/10.1210/en.2004-0985.CrossRefPubMed

27.

Kushiyama, A., Sakoda, H., Oue, N., Okubo, M., Nakatsu, Y., Ono, H., et al. (2013). Resistin-like molecule beta is abundantly expressed in foam cells and is involved in atherosclerosis development. Arteriosclerosis, Thrombosis, and Vascular Biology, 33(8), 1986–1993. https://doi.org/10.1161/ATVBAHA.113.301546.CrossRefPubMed

28.

Wang, J. M., Tao, J., Chen, D. D., Cai, J. J., Irani, K., Wang, Q., et al. (2014). MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus. Arteriosclerosis, Thrombosis, and Vascular Biology, 34(1), 99–109. https://doi.org/10.1161/ATVBAHA.113.302104.CrossRefPubMed

29.

Poliseno, L., Tuccoli, A., Mariani, L., Evangelista, M., Citti, L., Woods, K., et al. (2006). MicroRNAs modulate the angiogenic properties of HUVECs. Blood, 108(9), 3068–3071. https://doi.org/10.1182/blood-2006-01-012369.CrossRefPubMed

30.

Minami, Y., Satoh, M., Maesawa, C., Takahashi, Y., Tabuchi, T., Itoh, T., et al. (2009). Effect of atorvastatin on microRNA 221/222 expression in endothelial progenitor cells obtained from patients with coronary artery disease. European Journal of Clinical Investigation, 39(5), 359–367. https://doi.org/10.1111/j.1365-2362.2009.02110.x.CrossRefPubMed

31.

Liu, X., Cheng, Y., Zhang, S., Lin, Y., Yang, J., & Zhang, C. (2009). A necessary role of miR-221 and miR-222 in vascular smooth muscle cell proliferation and neointimal hyperplasia. Circulation Research, 104(4), 476–487. https://doi.org/10.1161/CIRCRESAHA.108.185363.CrossRefPubMedPubMedCentral

32.

Chen, T., Huang, Z., Wang, L., Wang, Y., Wu, F., Meng, S., et al. (2009). MicroRNA-125a-5p partly regulates the inflammatory response, lipid uptake, and ORP9 expression in oxLDL-stimulated monocyte/macrophages. Cardiovascular Research, 83(1), 131–139. https://doi.org/10.1093/cvr/cvp121.CrossRefPubMed

33.

Zhang, Q., Liu, L., & Zheng, X. Y. (2009). Protective roles of HDL, apoA-I and mimetic peptide on endothelial function: Through endothelial cells and endothelial progenitor cells. International Journal of Cardiology, 133(3), 286–292. https://doi.org/10.1016/j.ijcard.2008.11.034.CrossRefPubMed

34.

Patella, F., Leucci, E., Evangelista, M., Parker, B., Wen, J., Mercatanti, A., et al. (2013). MiR-492 impairs the angiogenic potential of endothelial cells. Journal of Cellular and Molecular Medicine, 17(8), 1006–1015. https://doi.org/10.1111/jcmm.12085.CrossRefPubMedPubMedCentral

35.

Adams, V., Linke, A., Krankel, N., Erbs, S., Gielen, S., Mobius-Winkler, S., et al. (2005). Impact of regular physical activity on the NAD(P)H oxidase and angiotensin receptor system in patients with coronary artery disease. Circulation, 111(5), 555–562. https://doi.org/10.1161/01.CIR.0000154560.88933.7E.CrossRefPubMed

36.

de Beer, V. J., Bender, S. B., Taverne, Y. J., Gao, F., Duncker, D. J., Laughlin, M. H., et al. (2011). Exercise limits the production of endothelin in the coronary vasculature. American Journal of Physiology. Heart and Circulatory Physiology, 300(5), H1950–H1959. https://doi.org/10.1152/ajpheart.00954.2010.CrossRefPubMedPubMedCentral

37.

Ekblom-Bak, E., Hellenius, M. L., Ekblom, O., Engstrom, L. M., & Ekblom, B. (2010). Independent associations of physical activity and cardiovascular fitness with cardiovascular risk in adults. European Journal of Cardiovascular Prevention and Rehabilitation : Official Journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology, 17(2), 175–180. https://doi.org/10.1097/HJR.0b013e32833254f2.CrossRef

38.

Andersen, L. B., Riddoch, C., Kriemler, S., & Hills, A. P. (2011). Physical activity and cardiovascular risk factors in children. British Journal of Sports Medicine, 45(11), 871–876. https://doi.org/10.1136/bjsports-2011-090333.CrossRefPubMed

39.

Gielen, S., Schuler, G., & Hambrecht, R. (2001). Exercise training in coronary artery disease and coronary vasomotion. Circulation, 103(1), E1–E6.CrossRef

40.

Perk, J., De Backer, G., Gohlke, H., Graham, I., Reiner, Z., Verschuren, M., et al. (2012). European Guidelines on cardiovascular disease prevention in clinical practice (version 2012). The Fifth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of nine societies and by invited experts). European Heart Journal, 33(13), 1635–1701. https://doi.org/10.1093/eurheartj/ehs092.CrossRefPubMed

Titel: Aerobic Exercise Prevents Insulin Resistance Through the Regulation of miR-492/Resistin Axis in Aortic Endothelium
verfasst von: Ying Cai
Kang-Ling Xie
Fan Zheng
Sui-Xin Liu
Publikationsdatum: 19.09.2018
Verlag: Springer US
Erschienen in: Journal of Cardiovascular Translational Research / Ausgabe 6/2018
Print ISSN: 1937-5387
Elektronische ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-018-9828-7

Neu im Fachgebiet Kardiologie

Screening-Mammografie offenbart erhöhtes Herz-Kreislauf-Risiko

26.04.2024 Mammografie Nachrichten

Routinemäßige Mammografien helfen, Brustkrebs frühzeitig zu erkennen. Anhand der Röntgenuntersuchung lassen sich aber auch kardiovaskuläre Risikopatientinnen identifizieren. Als zuverlässiger Anhaltspunkt gilt die Verkalkung der Brustarterien.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 Hypertonie Nachrichten

Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.

Adipositas-Medikament auch gegen Schlafapnoe wirksam

24.04.2024 Adipositas Nachrichten

Der als Antidiabetikum sowie zum Gewichtsmanagement zugelassene Wirkstoff Tirzepatid hat in Studien bei adipösen Patienten auch schlafbezogene Atmungsstörungen deutlich reduziert, informiert der Hersteller in einer Vorab-Meldung zum Studienausgang.

Update Kardiologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2018

Effect of Long-Term Exercise Training on lncRNAs Expression in the Vascular Injury of Insulin Resistance

Noncoding RNAs in Cardiac Hypertrophy

Protein Profiling in Serum and Cerebrospinal Fluid Following Complex Surgery on the Thoracic Aorta Identifies Biological Markers of Neurologic Injury

Development of a Novel Human Cell-Derived Tissue-Engineered Heart Valve for Transcatheter Aortic Valve Replacement: an In Vitro and In Vivo Feasibility Study