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01.12.2018 | Research article | Ausgabe 1/2018 Open Access

Arthritis Research & Therapy 1/2018

Age and fecal microbial strain-specific differences in patients with spondyloarthritis

Zeitschrift:
Arthritis Research & Therapy > Ausgabe 1/2018
Autoren:
Matthew L. Stoll, Pamela F. Weiss, Jennifer E. Weiss, Peter A. Nigrovic, Barbara S. Edelheit, S. Lou Bridges Jr, Maria I. Danila, Charles H. Spencer, Marilynn G. Punaro, Kenneth Schikler, Andreas Reiff, Ranjit Kumar, Randy Q. Cron, Casey D. Morrow, Elliot J. Lefkowitz
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s13075-018-1510-6) contains supplementary material, which is available to authorized users.

Abstract

Background

Prior studies have demonstrated abnormalities in the composition of the gastrointestinal microbiota in pediatric and adult patients with spondyloarthritis (SpA). In particular, diminished fecal abundance of Faecalibacterium prausnitzii and abnormalities in both directions in the abundance of the Bacteroides genus have been identified.

Methods

We obtained fecal specimens from 30 children with treatment-naïve enthesitis-related arthritis (ERA) and 19 healthy controls, as well as specimens from 11 adult patients with longstanding SpA and 10 adult healthy controls. All of the samples underwent sequencing of the 16S ribosomal DNA. A subset of the pediatric fecal samples was subjected to shotgun metagenomics sequencing.

Results

ERA patients had decreased abundance of the anti-inflammatory F. prausnitzii A2-165 strain (41 ± 28% versus 54 ± 20% of all sequences matching F. prausnitzii, p = 0.084) and an increased abundance of the control F. prausnitzii L2/6 strain (28 ± 28% versus 15 ± 15%, p = 0.038). Similar trends were observed in adults with longstanding SpA (n = 11) and controls (n = 10). In contrast, the fecal abundance of Bacteroides fragilis was increased in ERA subjects (2.0 ± 4.0% versus 0.45 ± 0.7% of all sequences, p = 0.045), yet was diminished in adult subjects (0.2 ± % versus 1.0 ± % of all sequences, p = 0.106). Shotgun metagenomics sequencing of the fecal DNA in the pediatric subjects revealed diminished coverage of the butanoate pathway (abundance normalized to controls of 1 ± 0.48 versus 0.72 ± 0.33 in ERA, p = 0.037).

Conclusions

The anti-inflammatory F. prausnitzii A2-165 strain appears to be depleted in both pediatric and adult SpA. In contrast, B. fragilis may be depleted in adult disease yet abundant in pediatric SpA, suggesting developmental effects on the immune system.
Zusatzmaterial
Additional file 1: Code for processing of 16S sequences with DADA2 and for shotgun sequence analysis with HUMAnN2. (DOCX 12 kb)
13075_2018_1510_MOESM1_ESM.docx
Literatur
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