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Comparative Clinical Pathology

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20.01.2021 | Original Article

Ameliorative effects of Garcinia hydroxybiflavanonol 1 (GB1) isolated from Garcinia kola seeds on cadmium chloride (CdCl₂) induced reproductive toxicity in the testis of the male Wistar rats

verfasst von: Clifford N. Abiaezute, Kenneth O. Anya, Edwin A. Uwagie-Ero, Chinaka O. Nwaehujor

Erschienen in: Comparative Clinical Pathology | Ausgabe 2/2021

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Abstract

A high association between cadmium, a common environmental pollutant, and infertility in males has been established. Studies have established that antioxidants can enhance fertility either directly or indirectly. Garcinia hydroxybiflavanonol (GB1) extracts from bitter kola is known for its natural antioxidant properties. This study is aimed at investigating the ameliorative effects of administration of (GB1) on the reproductive health of cadmium chloride (CdCl₂)–intoxicated male Wistar rats. Extraction, fractionation and isolation of Garcinia hydroxybiflavanonol (GB 1) were done from seeds of Garcinia kola. Thirty-six male adult Wistar rats weighing 170–190 g were acclimatized for 2 weeks and randomly divided into three groups. Group A were given distilled water as control, group B 2.5 mg/kg b.w. of CdCl₂ and group C 2.5 mg/kg b.w. of CdCl₂ and 2 mg/kg b.w. of GB1 dissolved in Tween20. The rats were orally dosed daily for 90 days. Every 30 days, 4 animals from each group were euthanized and blood samples collected for testosterone, GnRH, LH and FSH assay. Sperm qualities were determined using the cauda epididymis. The neutral-buffered formalin-fixed testes were processed for histology. The result showed significantly higher levels of testosterone, GnRH, FSH, LH and sperm quality in the CdCl₂ + GB1–treated rats than in the CdCl₂-only-treated rats. Histopathology showed progressive distortion, disorganisation, erosion and vacuolation of the seminiferous epithelium in CdCl₂-only-treated rats which were not observed in the other groups. These findings indicate a protective effect of the GB1 extract on the testes of Wistar rats.

Adaramoye OA, Arisekola M (2013) Kolaviron, a biflavonoid complex from Garcinia kola seeds, ameliorates ethanol-induced reproductive toxicity in male Wistar rats. Niger J Physiol Sci 28:009–015

Adaramoye OA, Farombi EO, Adeyemi EO, Emerole GO (2005) Inhibition of human low-density lipoprotein oxidation by flavonoids of Garcinia kola seeds. Pak J Med Sci 21:331–339

Adedara IA, Mathur PP, Farombi EO (2013) Kolaviron prevents ethylene glycol monoethyl ether-induced testicular apoptosis via down-regulation of stress proteins, Fas/Fas-L and caspases expressions in rats. Toxicol Mech Method 23:689–696CrossRef

Adesanya OA, Oluyemi KA, Ofusori IU, Omotuyi CO, Okwuonu A, Ukwenya AR (2007) Micromorphometric and stereological effects of ethanolic extracts of Garcinia cambogia seeds on the testes and epididymides of adult wistar rats. Intl J Alt Med 5(1):1–9

Aitken RJ, Roman DR (2008). Anti-oxidant systems and oxidative stress in the testes: in Cheng CY (ed) Molecular mechanisms in spermatogenesis. Landes Bioscience. Pp15-24

Akinloye O, Arowojolu AO, Shittu OB, Anetor JI (2006) Cadmium toxicity: a possible cause of male infertility in Nigeria. Reprod Biol 6:17–30PubMed

Akpantah AO, Oremosu AA, Ajala MO, Noronha CC, Okanlawon AO (2003) The effect of crude extract of Garcinia kola seed on the histology and hormonal milieu of male Sprague-Dawley rats’ reproductive organs. Niger J Health Biomed Sci 2:40–46

Akunna GG, Ogunmodede OS, Saalu CL, Ogunlade B, Bello AJ, Salawu EO (2012) Ameliorative effect of Moringa oleifera (drumstick) leaf extracts on chromium-induced testicular toxicity in rat testes. World J Life Sci Med Res 2:20–26

Akunna GG, Saalu LC, Ogunlade B, Enye LA (2014) Spermatotoxicity in animal models exposed to fragrance components. J Med Sci 14:46–50CrossRef

Alabi OK, Akomolafe RO, Olukiran OS, Adeyemi WJ (2017) The Garcinia kola biflavonoid kolaviron attenuates experimental hepatotoxicity induced by diclofenac. Pathophysiology 24:281–290PubMedCrossRef

Alkhedaide A, Alshehri ZS, Sabry A, Abdel-Ghaffar T, Soliman MM, Attia H (2016) Protective effect of grape seed extract against cadmium-induced testicular dysfunction. Mol Med Rep 13:3101–3109PubMedPubMedCentralCrossRef

Anetor JI (2002) Rising environmental cadmium levels in developing countries: threat to genome stability and health. Niger. J. Physiol. Sci. 27:103–115

Aoyagi T, Ishikawa H, Miyaji K, Hayakawa K, Hata M (2002) Cadmium-induced testicular damage in a rat model of subchronic intoxication. Reprod Med Biol 12:59–63CrossRef

Aprioku JS (2018) Investigation of the effects of ethanol seed extract of Garcinia kola on haematological and hepatorenal indices in Wistar albino rats. Int J Pharm Sci Res 9:975–980

Asuzu IU, Nwaehujor CO (2013) The anti-diabetic, hypolipidemic and anti-oxidant activities of D-3-O-methylchiroinositol in alloxan-induced diabetic rats. Hygeia J D Med 5:27–33

Benoff S, Auborn K, Marmar JL, Hurley IR (2008) Link between low-dose environmentally relevant cadmium exposures and asthenozoospermia in a rat model. Fertil Steril 89:73–79CrossRef

Chinnoch P (1996) Reprod health African Health 18:3–19

Doyle J, Pfander W, Grebing S, Pierce J (1974) Effect of dietary cadmium on growth, absorption and cadmium tissue levels in growing lambs. J Nutr 104:160–166PubMedCrossRef

Ebenebe CI, Okoli CA, Ogbu OC (2017) Reproductive traits of male weaner rabbits fed graded levels of Garcinia kola (bitter kola). Nig J Anim Sci 19:71–76

El-Demerdash FM, Yousef MI, Kedwany FS, Baghdadi HH (2004) Cadmium induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and b-carotene. Food Chem Toxicol 42:1563–1571PubMedCrossRef

Farombi EO, Adedara IA, Akinrinde SA, Ojo OO, Eboh AS (2012) Protective effects of kolaviron and quercetin on cadmium induced testicular damage and endocrine pathology in rats. Andrologia. 44:273–284PubMedCrossRef

Farombi O, Adedara I, Abarikwu S (2017) Chemopreventive activities of kolaviron, a novel bioflavonoid from the seed of Garcinia kola: mechanistic perspectives. Biochem Pharmacol 139:118CrossRef

Fasanya-odewumi C, Latinwo LM, Ikediobi CO, Giliard L, Sponholtz G, Nwoga J, Stino F, Hamilton N, Erdos GV (1998) The genotoxicity and cytotoxicity of dermally administered cadmium: effects of dermal cadmium administration. Int J Mol Med 1:1001–1006PubMed

Gallagher CM, Moonga BS, Kovach JS (2010) Cadmium, follicle-stimulating hormone, and effects on bone in women age 42–60 years, NHANES III. Environ Res 110:105–111PubMedCrossRef

Ganong WF (2003). Review of medical physiology. Lange medical book. McGraw-Hill Companies, USA. Pp. 429–430

Gollenberg AL, Hediger ML, Lee PA, Himes JH, Louis GM (2010). Association between lead and cadmium and reproductive hormones in peripubertal U.S. girls. Environ. Health Perspect 118: 1782–1787

Goyer RA, Liu J, Waalkes MP (2004) Cadmium and cancer of prostate and testis. Biometals 17:555–558PubMedCrossRef

Guyton AC, Hall JE (1998) Pocket companion to textbook of medical physiology, 9th edn. W.B. Saunders Company, India, pp 691–697

Habbeebu SS, Liu J, Klaassen CD (1998) Cadmium-induced apoptosis in mouse liver. Toxicol Appl Pharmacol 149:203–209CrossRef

Hachfi L, Sakly R (2010) Effect of cadmium transferred via food product on spermatogenesis in the rat. Andrologia 42:62–64PubMedCrossRef

Hew KW, Heath GL, Jiwa A, Welsh MJ (1993a) Cadmium in vivo causes disruption of tight junction-associated microfilaments in rat Sertoli cell. Biol Reprod 49:840–849PubMedCrossRef

Hew KW, Ericson WA, Welsh MJ (1993b) A single low cadmium dose causes failure of spermiation in the rat. Toxicol Appl Pharmacol 121:15–21PubMedCrossRef

Hoyer PB (2005) Damage to ovarian development and function. Cell Tissue Res 322:99–106PubMedCrossRef

International Register of Potentially Toxic Chemicals (IRPTC) legal files (1995). http://www.lavoisier.eu/books/agriculture/international-register-of-potentially-toxic- chemicals-irptc-legal-files-1994-1995-set-of-2-vols-et-user-s-guide-e-96-iii-d 1/description_2467506. Accessed September 20, 2017

Iwu MM, Igboko OA, Okunji CO, Tempesta MS (1990) Antidiabetic and aldose reductase activities of biflavanones of Garcnia kola. J Pharm Pharmacol 42:290–292PubMedCrossRef

Jackson LW, Howards PP, Wactawski-Wende J, Schisterman EF (2011) The association between cadmium, lead and mercury blood levels and reproductive hormones among healthy, premenopausal women. Hum Reprod 26:2887–2895PubMedPubMedCentralCrossRef

Jarup L, Berglund M, Elinder, C.G, Nordberg G, Vahter M (1998). Health effects of cadmium exposure: a review of the literature and risk estimate. Scand J Work Environ Health 24:1–52

Khanna S, Mitra S, Lakhera PC, Khandelwal S (2016) N-Acetylcysteine effectively mitigates cadmium-induced oxidative damage and cell death in Leydig cells in vitro. Drug Chem Toxicol 39:74–80PubMedCrossRef

Klimova S, Misurova E (2004) Effects of cadmium and ionizing radiationon histones in rat testes. Acta Vet Brno 73:483–489CrossRef

Koizumi T, Li ZG (1992) Role of oxidative stress in single-dose, cadmium-induced testicular cancer. J Toxicol Environ Health 37:25–36PubMedCrossRef

Koyama H, Kitoh H, Satoh M, Tohyama C (2002) Low dose exposure to cadmium and its health effects (1). Genotoxicity and carcinogenicity. Jpn J Hyg 57:547–555CrossRef

Lafuente A, Marquez N, Perez-Lorenzo M, Pazo D, Esquifino AI (2000) Pubertal and postpubertal cadmium exposure differentially affects the hypothalamic-pituitary-testicular axis function in the rat. Food Chem Toxicol 38:913–923PubMedCrossRef

Lafuente A, Cano P, Esquifino AI (2003) Are cadmium effects on plasmagonadotropins, prolactin, ACTH, GH and TSH levels, dose-dependent? Biometals 16:243–250PubMedCrossRef

Li J, Yi J, Wang C, Xu P (2000) Effect of cadmium on apoptosis of spermatogenic cells of rat testis and the protection effect of zinc against it. Wei Sheng Yan Jiu 30:135–137

Linares V, Belles M, Albina ML (2006) Assessment of the pro-oxidant activity of uranium in kidney and testis of rats. Toxicol Lett 167:152–161PubMedCrossRef

Lymberopoulos AG, Kotsaki-Kovatsi VP, Papaioannou N, Taylor A, Brikas P, Belibasaki S (2003) Effects of cadmium chloride administration on the testicular growth and plasma testosterone secretion of Chios ram-lambs. Small Rumin Res 49:51–60CrossRef

Madubunyi II (2010) Mechanism of hepatoprotective activities of Garcinia hydroxybiflavanonol, GB1. J Complement Integrat Med 7:1

Madubunyi II (2012) Effect of Garcinia hydroxybiflavanonols on protein synthesis in primary cultured rat hepatocytes. Comp Clin Path 21:137–142CrossRef

Marchlewicz M, Wiszniewska B, Gonet B (2007) Increased lipid peroxidation and ascorbic acid utilization in testis and epididymis of rats chronically exposed to lead. Biometals 20:13–19PubMedCrossRef

Marettová E, Maretta M, Legáth J (2010) Changes in the peritubular tissue of rat testis after cadmium treatment. Biol Trace Elem Res 134:288–295PubMedCrossRef

Marettová E, Maretta M, Legáth J (2013) Effect of cd with or without se supplementation on spermatogenesis and semen quality in the rooster (Gallus gallus). Avian Biol Res 6:275–280CrossRef

Massanyi P, Lukác N, Uhrin V, Toman R, Pivko J, Rafay J, Forgács Z, Somosy Z (2007) Female reproductive toxicology of cadmium. Acta Biol Hung 58:287–299PubMedCrossRef

Mendiola J, Moreno JM, Roca M, Vergara-Juarez N, Martinez-Garcia MJ (2011) Relationships between heavy metal concentrations in three different body fluids and male reproductive parameters: a pilot study. Environ Health 10:6PubMedPubMedCentralCrossRef

Mesembe GU, Asuquo OE, Fischer OR, Udoaffah VA (2013) Influence of long-term ingestion of Garcinia kola seed diet on sperm count. Sperm motility and fertility in the Wistar rat J Health Med Nurs 1:20–22

Mruk DD, Cheng CY (2011) Environmental contaminants. Is male reproductive health at risk? Spermatogenesis 1:1–8CrossRef

Nair AR, Lee WK, Smeets K, Swennen Q, Sanchez A, Thevenod F, Cuypers A (2015) Glutathione and mitochondria determine acute defence responses and adaptive processes in cadmium induced oxidative stress and toxicity of the kidney. Arch Toxicol 89:2273–2289PubMedCrossRef

Newairy AA, El-Sharaky AS, Badreldeen MM, Eweda SM, Sheweita SA (2007) The hepatoprotective effects of selenium against cadmium toxicity in rats. Toxicology 242:23–30PubMedCrossRef

Nna VU, Ujah GA, Mohamed M, Etim KB, Igba BO, Augustine ER, Osim EE (2017) Cadmium chloride–induced testicular toxicity in male Wistar rats; prophylactic effect of quercetin, and assessment of testicular recovery following cadmium chloride withdrawal. Biomed Pharmacother 94:109–123PubMedCrossRef

Nwaehujor CO, Nwinyi FC, Igile GO (2013) The wound healing activities of Garcinia hydroxybiflavanonol (GB1) from Garcinia kola in streptozotocin-induced diabetic rats. Int J Biochem Photon 108:281–287

Nwaehujor CO, Udegbunam RI, Ode JO, Udegbunam SO (2015) Analgesic anti-inflammatory anti-pyretic activities of Garcinia hydroxybiflavanonol (GB1) from Garcinia kola. J Korean Soc Appl Bi 58:91–96CrossRef

Olayinka ET, Ore A (2014). Kolaviron and L-ascorbic acid attenuate chlorambucil-induced testicular oxidative stress in rats. J Toxicol 2014, Article ID 587015, 1–9

Oluyemi KA, Jimoh R, Adesanya OA, Omotuyi IO, Josiah SJ, Oyesola TO (2007) Effects of crude ethanolic extract of Garcinia cambogia on the reproductive system of male wistar rats (Rattus novergicus). Afr J Biotec 6:1236–1238

Oyeyemi MO, Samuel GO, Ajayi TA, Adeniji DA (2011) Semen characteristics and sperm morphological studies of the West African Dwarf buck treated with Aloe vera gel extract. Iranian J Reprod Med 9:83–88

Pajavic SB, Saicic ZS (2008) Modulation of antioxidant enzymes activities by sexual steroid hormone. Physiol Res 57:801–811CrossRef

Pant N, Kumar G, Upadhyay AD, Gupta YK, Chaturvedi PK (2015) Correlation between lead and cadmium concentration and semen quality. Andrologia. 47:887–891PubMed

Reddy KP, Madhu P, Reddy PS (2016) Protective effects of resveratrol against cisplatin-induced testicular and epididymal toxicity in rats. Food Chem Toxicol 91:65–72PubMedCrossRef

Renugadevi J, Prabu SM (2009) Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology. 256:128–134PubMedCrossRef

Roychoudhury S, Massanyi P, Bulla J, Choudhury MD, Lukac N (2010). Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrity in vitro. J. Environ. Sci. Health Part A, 45: 1374–1383

Santos FW, Oro T, Zeni G (2004) Cadmium induced testicular damage and its response to administration of succimer and diphenyl diselenide in mice. Toxicol Lett 152:255–263PubMedCrossRef

Sewani-Rusike CR, Ralebona N, Nkeh-Chungag BN (2016) Dose and time dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats. Andrologia 48:300–307PubMedCrossRef

Takiguchi M, Yoshihara S (2006) New aspects of cadmium as endocrine disruptor. Environ Sci 13:107–116PubMed

Tietz NW (1995). Clinical guide to laboratory tests. Section I: general clinical tests 3rd ed. Philadelphia: WB Saunders Company, Pp 66-71

Thompson J, Bannigan J (2008) Cadmium: toxic effects on the reproductive system and the embryo. Reprod Toxicol 25:304–315PubMedCrossRef

Toman R, Massanyi P, Uhrin V (2002) Changes in the testis and epididymis of rabbits after an intraperitoneal and peroral administration of cadmium. Trace Elem Electroly 19:114–117

Uwagie-Ero EA, Nwaehujor CO, Abiaezute CN, Ocheja OB, Ekeolu KB, Asuzu SI (2018) D-3-O-Methylchiroinositol (from Pilostigma thonningii) ameliorates cadmium chloride (CdCl₂)-induced toxicity in male reproduction. J Appl Sci Environ Manage 22:853–856

Valko MMHCM, Morris H, Cronin MTD (2005) Metals, toxicity and oxidative stress. Curr Med Chem 12:1161–1208PubMedCrossRef

Waalkes MP (2000) Cadmium carcinogenesis in review. J Inorg Biochem 79:241–244PubMedCrossRef

Waye A, Trudeau VL (2011) Neuroendocrine disruption: more than hormones are upset. J Toxicol Environ Health B Crit Rev 14:270–291PubMedPubMedCentralCrossRef

World Health Organization (1999) WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge University Press, Cambridge

Xu CY, Johnson JE, Singh PK, Jones MM, Yan H, Carter CE (1996) In vivo studies of cadmium induced apoptosis in testicular tissue of the rat and its modulation by a chelating agent. Toxicol. 107:1–8CrossRef

Yan H, Carter C, Xu C, Singh PK, Jones MM, Johnson JE, Dietrich MS (1997) Cadmium-induced apoptosis in the urogenital organs of the male rat and its suppression by chelaton. J Toxicol Environ Health 52:149–168PubMedCrossRef

Zhou T, Zhou G, Song W, Eguchi N, Lu W, Lundin E, Jin T, Nord-berg G (1999) Cadmium-induced apoptosis and changes in expression of p53, C-jun and Mt-i genes in testes and ventral prostate of rats. Toxicology 142:1–13PubMedCrossRef

Zhou T, Jia X, Chapin RE, Maronpot RR, Harris MW, Liu J, Waalkes MP, Eddy EM (2004) Cadmium at a non-toxic dose alters gene expression in mouse testes. Toxicol Lett 154:191–200PubMedCrossRef

Titel: Ameliorative effects of Garcinia hydroxybiflavanonol 1 (GB1) isolated from Garcinia kola seeds on cadmium chloride (CdCl2) induced reproductive toxicity in the testis of the male Wistar rats
verfasst von: Clifford N. Abiaezute
Kenneth O. Anya
Edwin A. Uwagie-Ero
Chinaka O. Nwaehujor
Publikationsdatum: 20.01.2021
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 2/2021
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-021-03201-8

Springer Medizin

Ameliorative effects of Garcinia hydroxybiflavanonol 1 (GB1) isolated from Garcinia kola seeds on cadmium chloride (CdCl₂) induced reproductive toxicity in the testis of the male Wistar rats

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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