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01.07.2016 | Original Investigation

Analysis of an echovirus 18 outbreak in Thuringia, Germany: insights into the molecular epidemiology and evolution of several enterovirus species B members

verfasst von: Andi Krumbholz, Renate Egerer, Heike Braun, Michaela Schmidtke, Dagmar Rimek, Claudia Kroh, Bert Hennig, Marco Groth, Andreas Sauerbrei, Roland Zell

Erschienen in: Medical Microbiology and Immunology | Ausgabe 5/2016

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Abstract

In October and November 2010, six children and one woman were presented with symptoms of aseptic meningitis in Jena, Thuringia, Germany. Enterovirus RNA was detected in the cerebrospinal fluid of all patients by RT-PCR, and preliminary molecular typing revealed echovirus 18 (E-18) as causative agent. Virus isolates were obtained from stool samples of three patients and several contact persons. Again, most isolates were typed as E-18. In addition, coxsackievirus B5 (CV-B5) and echovirus 25 (E-25) were found to co-circulate. As only few complete E-18 sequences are available in GenBank, the entire genomes of these isolates were determined using direct RNA-sequencing technology. We did not find evidence for recombination between E-18, E-25 or CV-B5 during the outbreak. Viral protein 1 gene sequences and the cognate 3D polymerase gene sequences of each isolate and GenBank sequences were analysed in order to define type-specific recombination groups (recogroups).

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Titel: Analysis of an echovirus 18 outbreak in Thuringia, Germany: insights into the molecular epidemiology and evolution of several enterovirus species B members
verfasst von: Andi Krumbholz
Renate Egerer
Heike Braun
Michaela Schmidtke
Dagmar Rimek
Claudia Kroh
Bert Hennig
Marco Groth
Andreas Sauerbrei
Roland Zell
Publikationsdatum: 01.07.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Medical Microbiology and Immunology / Ausgabe 5/2016
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI: https://doi.org/10.1007/s00430-016-0464-z

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