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Archives of Virology

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01.11.2014 | Brief Report

Analysis of small RNAs derived from Chinese wheat mosaic virus

verfasst von: Jian Yang, Shi-Ling Zheng, Heng-Mu Zhang, Xiao-Ya Liu, Jing Li, Jun-Min Li, Jian-Ping Chen

Erschienen in: Archives of Virology | Ausgabe 11/2014

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Abstract

The virus-derived small interfering RNAs (vsiRNAs) of Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus, were characterised from wheat plants by deep sequencing. CWMV vsiRNAs of 21–22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CWMV-infected wheat plants. The 5′-terminal base of vsiRNAs was biased towards A/U, suggesting that CWMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were predicted.

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Titel: Analysis of small RNAs derived from Chinese wheat mosaic virus
verfasst von: Jian Yang
Shi-Ling Zheng
Heng-Mu Zhang
Xiao-Ya Liu
Jing Li
Jun-Min Li
Jian-Ping Chen
Publikationsdatum: 01.11.2014
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 11/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2155-7

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