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01.12.2014 | Research | Ausgabe 1/2014 Open Access

Chinese Medicine 1/2014

Anti-infective and cytotoxic properties of Bupleurum marginatum

Zeitschrift:
Chinese Medicine > Ausgabe 1/2014
Autoren:
Mohamed L Ashour, Mahmoud Z El-Readi, Razan Hamoud, Safaa Y Eid, Sherweit H El Ahmady, Endalkachew Nibret, Florian Herrmann, Mahmoud Youns, Ahmed Tahrani, Dorothea Kaufmann, Michael Wink
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1749-8546-9-4) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

MLA and MW designed the study. MLA, MZL, RH, SYE, SHE, EN, FH, MY, AT and DKperformed the experiments. MLA and MW wrote the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Bupleurum marginatum Wall. ex DC (Apiaceae) is a perennial herb widely used in traditional Chinese and Kampo medicine for the treatment of various infectious diseases. The biological activities of B. marginatum have not been fully investigated. This study aims to investigate the antitrypanosomal, antimicrobial and antiviral activities of methanol (ME) and dichloromethane (DCM) extracts of B. marginatum aerial parts and the ability of both extracts to inhibit the growth of different cancer cell lines.

Methods

Phytochemical characterization of the extracts was performed by LC-MS profiling. The antitrypanosomal activity was evaluated using the resazurin method. The antimicrobial activity was assessed using agar diffusion and microdilution methods, and the minimum inhibitory concentration (MIC) values were determined. The antiviral activity was determined for 6.25, 12.5, and 50 μg/mL doses using a plaque reduction assay. Cytotoxicity was investigated in eight cancer cell lines (Caco-2, CCL-81, CCRF-CEM, COS-7, HL-60, MIA PaCa-2, MCF-7, and PANC-1) using the MTT assay and the caspase 3/7 activity was determined over the range of 62.5–1000 μg/mL.

Results

Phytochemical analyses resulted in the characterization of 15 components, mainly flavonoids and lignans. The DCM extract showed significant antitrypanosomal activity (IC50: 36.21 μg/mL) and moderate activity against Streptococcus pyogenes (MIC value: 0.25 mg/mL). At a dose of 12.5 μg/mL, the DCM extract inhibited 73.6% of the plaque production by hepatitis A virus. CCRF-CEM cells were the most sensitive to both extracts (IC50: 12.5–22.7 μg/mL). The cytotoxicity was mediated by induction of apoptosis (19-fold increase in the cellular caspase 3/7 level after treatment with the DCM extract at 1 mg/mL).

Conclusions

ME and DCM extract of B. marginatum showed anti-infective and antiproliferative effects.
Zusatzmaterial
Authors’ original file for figure 1
13020_2013_199_MOESM1_ESM.tiff
Authors’ original file for figure 2
13020_2013_199_MOESM2_ESM.pdf
Authors’ original file for figure 3
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Authors’ original file for figure 4
13020_2013_199_MOESM4_ESM.tif
Literatur
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