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Archives of Virology

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01.09.2008 | Original Article

Antigenic characterization of bovine ephemeral fever rhabdovirus G and G_NS glycoproteins expressed from recombinant baculoviruses

verfasst von: Jasjit Johal, Karryn Gresty, Kritaya Kongsuwan, Peter J. Walker

Erschienen in: Archives of Virology | Ausgabe 9/2008

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Abstract

Recombinant baculoviruses expressing the BEFV envelope glycoprotein G and non-structural glycoprotein G_NS were constructed. The G protein expressed in insect cells was located on the cell surface and induced spontaneous cell fusion at mildly acidic pH. The expressed G protein reacted with MAbs to continuous and conformational neutralization sites (G1, G2, G3b and G4), but not to conformational site G3a. The expressed G_NS protein was also located on the cell surface but did not exhibit fusogenic activity. The G_NS protein reacted with polyclonal antiserum produced from vaccinia-virus-expressed recombinant G_NS but did not react with G protein antibodies. A His₆-tagged, soluble form of the G protein was expressed and purified by Ni²⁺–NTA chromatography. The purified G protein reacted with BEFV-neutralizing MAbs to all continuous and conformational antigenic sites. The highly protective characteristics of the native BEFV G protein suggest that the secreted, baculovirus-expressed product may be a useful vaccine antigen.

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Titel: Antigenic characterization of bovine ephemeral fever rhabdovirus G and GNS glycoproteins expressed from recombinant baculoviruses
verfasst von: Jasjit Johal
Karryn Gresty
Kritaya Kongsuwan
Peter J. Walker
Publikationsdatum: 01.09.2008
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 9/2008
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-008-0164-0

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