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Erschienen in: Oral and Maxillofacial Surgery 4/2020

11.07.2020 | Original Article

Bone healing at collagenated bicortically installed implants: an experimental study in rabbits

verfasst von: Luigi Feletto, Franco Bengazi, Joaquín Juan Urbizo Velez, Mauro Ferri, Riccardo Favero, Daniele Botticelli

Erschienen in: Oral and Maxillofacial Surgery | Ausgabe 4/2020

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Abstract

Purpose

To study the healing at collagenated bicortically installed implants.

Methods

Twenty albino New Zealand rabbits were used for implant installation. Two implants with a double acid etched surface, coated with a collagen type I or left uncoated, were installed bicortically in the metaphysis and in the diaphysis of each tibia. Ten rabbits were euthanized after 2 weeks and ten after 6 weeks after installation. Ground sections were prepared for histological analyses that were performed both in the cortical layers and in the marrow regions.

Results

After 2 weeks of healing, highest amounts of new bone were found at the collagenated implants (43.2 ± 6.0%) compared to the standard implants (33.9 ± 6.1%; p = 0.022). After 6 weeks of healing, similar percentages of new bone were observed, being 51.8 ± 7.3% and 50.9 ± 9.6% (p = 0.678) for the standard and collagenated surfaces, respectively.

Conclusions

A coated surface with collagen type I promoted bone apposition in the earliest periods of healing. However, the effect vanished over time so that similar results were obtained after 6 weeks of healing.
Literatur
1.
Zurück zum Zitat Botticelli D, Lang NP (2017) Dynamics of osseointegration in various human and animal models - a comparative analysis. Clin Oral Implants Res 28(6):742–748PubMed Botticelli D, Lang NP (2017) Dynamics of osseointegration in various human and animal models - a comparative analysis. Clin Oral Implants Res 28(6):742–748PubMed
2.
Zurück zum Zitat Abrahamsson I, Berglundh T, Linder E, Lang NP, Lindhe J (2004) Early bone formation adjacent to rough and turned endosseous implant surfaces. An experimental study in the dog. Clin Oral Implants Res 15(4):381–392PubMed Abrahamsson I, Berglundh T, Linder E, Lang NP, Lindhe J (2004) Early bone formation adjacent to rough and turned endosseous implant surfaces. An experimental study in the dog. Clin Oral Implants Res 15(4):381–392PubMed
3.
Zurück zum Zitat Rossi F, Lang NP, De Santis E, Morelli F, Favero G, Botticelli D (2014) Bone-healing pattern at the surface of titanium implants: an experimental study in the dog. Clin Oral Implants Res 25(1):124–131PubMed Rossi F, Lang NP, De Santis E, Morelli F, Favero G, Botticelli D (2014) Bone-healing pattern at the surface of titanium implants: an experimental study in the dog. Clin Oral Implants Res 25(1):124–131PubMed
4.
Zurück zum Zitat Wennerberg A, Albrektsson T (2009) Effects of titanium surface topography on bone integration: a systematic review. Clin Oral Implants Res 20(Suppl 4):172–184PubMed Wennerberg A, Albrektsson T (2009) Effects of titanium surface topography on bone integration: a systematic review. Clin Oral Implants Res 20(Suppl 4):172–184PubMed
5.
Zurück zum Zitat Nagassa ME, Daw AE, Rowe WG, Carley A, Thomas DW, Moseley R (2008) Optimisation of the hydrogen peroxide pre-treatment of titanium: surface characterisation and protein adsorption. Clin Oral Implants Res 19(12):1317–1326PubMed Nagassa ME, Daw AE, Rowe WG, Carley A, Thomas DW, Moseley R (2008) Optimisation of the hydrogen peroxide pre-treatment of titanium: surface characterisation and protein adsorption. Clin Oral Implants Res 19(12):1317–1326PubMed
6.
Zurück zum Zitat Junker R, Dimakis A, Thoneick M, Jansen JA (2009) Effects of implant surface coatings and composition on bone integration: a systematic review. Clin Oral Implants Res 20(Suppl 4):185–206PubMed Junker R, Dimakis A, Thoneick M, Jansen JA (2009) Effects of implant surface coatings and composition on bone integration: a systematic review. Clin Oral Implants Res 20(Suppl 4):185–206PubMed
7.
Zurück zum Zitat Sawase T, Wennerberg A, Hallgren C, Albrektsson T, Baba K (2000) Chemical and topographical surface analysis of five different implant abutments. Clin Oral Implants Res 11(1):44–50PubMed Sawase T, Wennerberg A, Hallgren C, Albrektsson T, Baba K (2000) Chemical and topographical surface analysis of five different implant abutments. Clin Oral Implants Res 11(1):44–50PubMed
8.
Zurück zum Zitat Tugulu S, Löwe K, Scharnweber D, Schlottig F (2010) Preparation of superhydrophilic microrough titanium implant surfaces by alkali treatment. J Mater Sci Mater Med 21(10):2751–2763PubMed Tugulu S, Löwe K, Scharnweber D, Schlottig F (2010) Preparation of superhydrophilic microrough titanium implant surfaces by alkali treatment. J Mater Sci Mater Med 21(10):2751–2763PubMed
9.
Zurück zum Zitat Rupp F, Scheideler L, Olshanska N, de Wild M, Wieland M, Geis-Gerstorfer J (2006) Enhancing surface free energy and hydrophilicity through chemical modification of microstructured titanium implant surfaces. J Biomed Mater Res A 76(2):323–334PubMed Rupp F, Scheideler L, Olshanska N, de Wild M, Wieland M, Geis-Gerstorfer J (2006) Enhancing surface free energy and hydrophilicity through chemical modification of microstructured titanium implant surfaces. J Biomed Mater Res A 76(2):323–334PubMed
10.
Zurück zum Zitat Canullo L, Genova T, Tallarico M, Gautier G, Mussano F, Botticelli D (2016) Plasma of argon affects the earliest biological response of different implant surfaces: an in vitro comparative study. J Dent Res 95(5):566–573PubMed Canullo L, Genova T, Tallarico M, Gautier G, Mussano F, Botticelli D (2016) Plasma of argon affects the earliest biological response of different implant surfaces: an in vitro comparative study. J Dent Res 95(5):566–573PubMed
11.
Zurück zum Zitat Coelho PG, Giro G, Teixeira HS, Marin C, Witek L, Thompson VP, Tovar N, Silva NR (2012) Argon-based atmospheric pressure plasma enhances early bone response to rough titanium surfaces. J Biomed Mater Res A 100(7):1901–1906PubMed Coelho PG, Giro G, Teixeira HS, Marin C, Witek L, Thompson VP, Tovar N, Silva NR (2012) Argon-based atmospheric pressure plasma enhances early bone response to rough titanium surfaces. J Biomed Mater Res A 100(7):1901–1906PubMed
12.
Zurück zum Zitat Duske K, Koban I, Kindel E, Schröder K, Nebe B, Holtfreter B, Jablonowski L, Weltmann KD, Kocher T (2012) Atmospheric plasma enhances wettability and cell spreading on dental implant metals. J Clin Periodontol 39(4):400–407PubMed Duske K, Koban I, Kindel E, Schröder K, Nebe B, Holtfreter B, Jablonowski L, Weltmann KD, Kocher T (2012) Atmospheric plasma enhances wettability and cell spreading on dental implant metals. J Clin Periodontol 39(4):400–407PubMed
13.
Zurück zum Zitat Beolchini M, Lang NP, Gómez Moreno G, Iezzi G, Botticelli D, Calvo Guirado JL (2016) Bone healing at implants with different surface configurations: an experimental study in dogs. Clin Oral Implants Res 27(2):196–202PubMed Beolchini M, Lang NP, Gómez Moreno G, Iezzi G, Botticelli D, Calvo Guirado JL (2016) Bone healing at implants with different surface configurations: an experimental study in dogs. Clin Oral Implants Res 27(2):196–202PubMed
14.
Zurück zum Zitat Caneva M, Lang NP, Calvo Guirado JL, Spriano S, Iezzi G, Botticelli D (2015) Bone healing at bicortically installed implants with different surface configurations. An experimental study in rabbits. Clin Oral Implants Res 26(3):293–299PubMed Caneva M, Lang NP, Calvo Guirado JL, Spriano S, Iezzi G, Botticelli D (2015) Bone healing at bicortically installed implants with different surface configurations. An experimental study in rabbits. Clin Oral Implants Res 26(3):293–299PubMed
16.
Zurück zum Zitat Regazzoni C, Winterhalter KH, Rohrer L (2001) Type I collagen induces expression of bone morphogenetic protein receptor type II. Biochem Biophys Res Commun 283(2):316–322CrossRef Regazzoni C, Winterhalter KH, Rohrer L (2001) Type I collagen induces expression of bone morphogenetic protein receptor type II. Biochem Biophys Res Commun 283(2):316–322CrossRef
17.
Zurück zum Zitat Morra M, Cassinelli C, Cascardo G, Cahalan P, Cahalan L, Fini M, Giardino R (2003) Surface engineering of titanium by collagen immobilization. Surface characterization and in vitro and in vivo studies. Biomaterials. 24(25):4639–4654CrossRef Morra M, Cassinelli C, Cascardo G, Cahalan P, Cahalan L, Fini M, Giardino R (2003) Surface engineering of titanium by collagen immobilization. Surface characterization and in vitro and in vivo studies. Biomaterials. 24(25):4639–4654CrossRef
18.
Zurück zum Zitat Morra M, Cassinelli C, Cascardo G, Mazzucco L, Borzini P, Fini M, Giavaresi G, Giardino R (2006) Collagen I-coated titanium surfaces: mesenchymal cell adhesion and in vivo evaluation in trabecular bone implants. J Biomed Mater Res A 78(3):449–458CrossRef Morra M, Cassinelli C, Cascardo G, Mazzucco L, Borzini P, Fini M, Giavaresi G, Giardino R (2006) Collagen I-coated titanium surfaces: mesenchymal cell adhesion and in vivo evaluation in trabecular bone implants. J Biomed Mater Res A 78(3):449–458CrossRef
19.
Zurück zum Zitat Munisamy S, Vaidyanathan TK, Vaidyanathan J (2008) A bone-like precoating strategy for implants: collagen immobilization and mineralization on pure titanium implant surface. J Oral Implantol 34(2):67–75CrossRef Munisamy S, Vaidyanathan TK, Vaidyanathan J (2008) A bone-like precoating strategy for implants: collagen immobilization and mineralization on pure titanium implant surface. J Oral Implantol 34(2):67–75CrossRef
20.
Zurück zum Zitat Morra M, Cassinelli C, Cascardo G, Bollati D, Rodriguez Y, Baena R (2010) Multifunctional implant surfaces: surface characterization and bone response to acid-etched Ti implants surface-modified by fibrillar collagen I. J Biomed Mater Res A 94(1):271–279PubMed Morra M, Cassinelli C, Cascardo G, Bollati D, Rodriguez Y, Baena R (2010) Multifunctional implant surfaces: surface characterization and bone response to acid-etched Ti implants surface-modified by fibrillar collagen I. J Biomed Mater Res A 94(1):271–279PubMed
21.
Zurück zum Zitat Morra M, Cassinelli C, Cascardo G, Bollati D, Baena RR (2011) Gene expression of markers of osteogenic differentiation of human mesenchymal cells on collagen I-modified microrough titanium surfaces. J Biomed Mater Res A 96(2):449–455PubMed Morra M, Cassinelli C, Cascardo G, Bollati D, Baena RR (2011) Gene expression of markers of osteogenic differentiation of human mesenchymal cells on collagen I-modified microrough titanium surfaces. J Biomed Mater Res A 96(2):449–455PubMed
22.
Zurück zum Zitat Sartori M, Giavaresi G, Parrilli A, Ferrari A, Aldini NN, Morra M, Cassinelli C, Bollati D, Fini M (2015) Collagen type I coating stimulates bone regeneration and osteointegration of titanium implants in the osteopenic rat. Int Orthop 39(10):2041–2052PubMed Sartori M, Giavaresi G, Parrilli A, Ferrari A, Aldini NN, Morra M, Cassinelli C, Bollati D, Fini M (2015) Collagen type I coating stimulates bone regeneration and osteointegration of titanium implants in the osteopenic rat. Int Orthop 39(10):2041–2052PubMed
23.
Zurück zum Zitat Costa DG, Ferraz EP, Abuna RPF, de Oliveira PT, Morra M, Beloti MM, Rosa AL (2017) The effect of collagen coating on titanium with nanotopography on in vitro osteogenesis. J Biomed Mater Res A 105(10):2783–2788PubMed Costa DG, Ferraz EP, Abuna RPF, de Oliveira PT, Morra M, Beloti MM, Rosa AL (2017) The effect of collagen coating on titanium with nanotopography on in vitro osteogenesis. J Biomed Mater Res A 105(10):2783–2788PubMed
25.
Zurück zum Zitat Sverzut AT, Crippa GE, Morra M, de Oliveira PT, Beloti MM, Rosa AL (2012) Effects of type I collagen coating on titanium osseointegration: histomorphometric, cellular and molecular analyses. Biomed Mater 7(3):035007PubMed Sverzut AT, Crippa GE, Morra M, de Oliveira PT, Beloti MM, Rosa AL (2012) Effects of type I collagen coating on titanium osseointegration: histomorphometric, cellular and molecular analyses. Biomed Mater 7(3):035007PubMed
26.
Zurück zum Zitat de Assis AF, Beloti MM, Crippa GE, de Oliveira PT, Morra M, Rosa AL (2009) Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface. Clin Oral Implants Res 20(3):240–246PubMed de Assis AF, Beloti MM, Crippa GE, de Oliveira PT, Morra M, Rosa AL (2009) Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface. Clin Oral Implants Res 20(3):240–246PubMed
Metadaten
Titel
Bone healing at collagenated bicortically installed implants: an experimental study in rabbits
verfasst von
Luigi Feletto
Franco Bengazi
Joaquín Juan Urbizo Velez
Mauro Ferri
Riccardo Favero
Daniele Botticelli
Publikationsdatum
11.07.2020
Verlag
Springer Berlin Heidelberg
Erschienen in
Oral and Maxillofacial Surgery / Ausgabe 4/2020
Print ISSN: 1865-1550
Elektronische ISSN: 1865-1569
DOI
https://doi.org/10.1007/s10006-020-00882-3

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