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Graefe's Archive for Clinical and Experimental Ophthalmology

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01.11.2003 | Laboratory Investigation

Cathepsin B in the rat eye

verfasst von: Johan Wassélius, Hanna Wallin, Magnus Abrahamson, Berndt Ehinger

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 11/2003

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Abstract

Background

Cathepsin B is a mammalian cysteine protease. The enzyme has been suggested to participate in the patophysiological processes of keratoconus as well as in the corneal response to infectious agents. This study describes the localization of cathepsin B in the rat eye.

Methods

Cathepsin B was identified in rat ocular tissues by Western blotting and immunohistochemistry. Cathepsin B mRNA levels were analyzed in the tissues by quantitative real-time cDNA amplification (QRT-PCR).

Results

Cathepsin B is present in the epithelium, in stromal cells and in the endothelium of the cornea. It is also present in the epithelium lining the ciliary processes, in occasional stromal cells in the iris, in the anterior subcapsular lens epithelium and in various cell types in the retina. At all locations cathepsin B is present in cytoplasmic granules, presumably lysosomes. QRT-PCR analysis detected cathepsin B mRNA in all these tissues in amounts correlating to the immunodetection results, suggesting that the enzyme detected is locally produced.

Conclusions

Cathepsin B is present in several tissues and cell types throughout the rat eye. It is localized to cytoplasmic granules, presumably lysosomes. Our results suggest that it is probably also produced in the same cell types.

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Titel: Cathepsin B in the rat eye
verfasst von: Johan Wassélius
Hanna Wallin
Magnus Abrahamson
Berndt Ehinger
Publikationsdatum: 01.11.2003
Verlag: Springer-Verlag
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 11/2003
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-003-0782-x

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Springer Medizin

Cathepsin B in the rat eye

Abstract

Background

Methods

Results

Conclusions

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Springer Medizin

Abstract

Background

Methods

Results

Conclusions

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