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Irish Journal of Medical Science (1971 -)

Erschienen in:

03.05.2017 | Original Article

CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma

verfasst von: W. Wu, B. Ouyang, Z. Lu, H. Liu, Y. Tan, P. Cui

Erschienen in: Irish Journal of Medical Science (1971 -) | Ausgabe 4/2017

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Abstract

Background

CCK1 (cholecystokinin receptor 1) and protein lysine acetylation were associated with several cancers, respectively. However, whether they are involved in the alternation of gallbladder carcinoma is unknown.

Aims

This study investigated the characteristics of CCK1 and protein lysine acetylation in GBC-SD cells and carcinoma of gallbladder.

Methods

The expression and localization of CCK1 were detected by western blot analysis and indirect immunofluorescence. GBC-SD cells were treated with CCK-8 and CCK-8+CCK1 inhibitor. The protein lysine acetylation from cells, as well as tissues of gallbladder carcinoma, was examined by western blotting.

Results

CCK1 receptor was expressed and localized in the GBC-SD cells. The synthetic octapeptide of CCK (CCK-8) could accelerate the lysine acetylation of a subset of proteins in dose-dependent manners in GBC-SD cells. Further investigation demonstrated that the specific inhibitor (CR1409) of CCK1 receptor could attenuate the CCK8-induced increase of protein lysine acetylation. In addition, we revealed that the rise of CCK1 receptor expression is associated with the increase of protein lysine acetylation in tissues from carcinoma of gallbladder.

Conclusions

CCK might regulate protein lysine acetylation via CCK1 receptor.

Chandra R, Liddle RA (2007) Cholecystokinin. Curr Opin Endocrinol Diabetes Obes 14:63–67CrossRefPubMed

Crawley JN, Corwin RL (1994) Biological actions of cholecystokinin. Peptides 15:731–755CrossRefPubMed

Mutt V (1994) Historical perspectives on cholecystokinin research. Ann N Y Acad Sci 713:1–10CrossRefPubMed

Vanhoutte PM, Humphrey PP, Spedding M (1996) X. International Union of Pharmacology recommendations for nomenclature of new receptor subtypes. Pharmacol Rev 48:1–2PubMed

Honda T, Wada E, Battey JF, Wank SA (1993) Differential gene expression of CCK(A) and CCK(B) receptors in the rat brain. Mol Cell Neurosci 4:143–154CrossRefPubMed

Wank SA (1995) Cholecystokinin receptors. Am J Phys 269:G628–G646

Matsusue K, Takiguchi S, Takata Y, Funakoshi A, Miyasaka K et al (1999) Expression of cholecystokinin type A receptor gene correlates with DNA demethylation during postnatal development of rat pancreas. Biochem Biophys Res Commun 264:29–32CrossRefPubMed

Hughes J, Boden P, Costall B, Domeney A, Kelly E et al (1990) Development of a class of selective cholecystokinin type B receptor antagonists having potent anxiolytic activity. Proc Natl Acad Sci U S A 87:6728–6732CrossRefPubMedPubMedCentral

Miyasaka K, Kanai S, Ohta M, Kawanami T, Kono A et al (1994) Lack of satiety effect of cholecystokinin (CCK) in a new rat model not expressing the CCK-A receptor gene. Neurosci Lett 180:143–146CrossRefPubMed

10.

Miyasaka K, Kobayashi S, Ohta M, Kanai S, Yoshida Y et al (2002) Anxiety-related behaviors in cholecystokinin-A, B, and AB receptor gene knockout mice in the plus-maze. Neurosci Lett 335:115–118CrossRefPubMed

11.

Rehfeld JF (2016) Cholecystokinin expression in tumors: biogenetic and diagnostic implications. Future Oncol 12:2135–2147CrossRefPubMed

12.

Mandair KK, Towner P, Stamford IF, Morris JD, Harper E et al (1998) Cholecystokinin receptors in human pancreatic cancer cell lines. Eur J Cancer 34:1455–1459CrossRefPubMed

13.

Rai R, Chandra V, Tewari M, Kumar M, Shukla HS (2012) Cholecystokinin and gastrin receptors targeting in gastrointestinal cancer. Surg Oncol 21:281–292CrossRefPubMed

14.

Smith JP, Solomon TE (2014) Cholecystokinin and pancreatic cancer: the chicken or the egg? Am J Physiol Gastrointest Liver Physiol 306:G91–G101CrossRefPubMed

15.

Piehler JM, Crichlow RW (1978) Primary carcinoma of the gallbladder. Surg Gynecol Obstet 147:929–942PubMed

16.

Ho WL, Hsu WM, Huang MC, Kadomatsu K, Nakagawara A (2016) Protein glycosylation in cancers and its potential therapeutic applications in neuroblastoma. J Hematol Oncol 9:100CrossRefPubMedPubMedCentral

17.

Li NY, Weber CE, Mi Z, Wai PY, Cuevas BD et al (2013) Osteopontin up-regulates critical epithelial-mesenchymal transition transcription factors to induce an aggressive breast cancer phenotype. J Am Coll Surg 217:17–26 discussion 26 CrossRefPubMed

18.

Iyer NG, Chin SF, Ozdag H, Daigo Y, Hu DE et al (2004) p300 regulates p53-dependent apoptosis after DNA damage in colorectal cancer cells by modulation of PUMA/p21 levels. Proc Natl Acad Sci U S A 101:7386–7391CrossRefPubMedPubMedCentral

19.

Zhou Y, Ru Y, Shi H, Wang Y, Wu B et al (2015) Cholecystokinin receptors regulate sperm protein tyrosine phosphorylation via uptake of HCO3. Reproduction 150:257–268CrossRefPubMed

20.

Yu H, Diao H, Wang C, Lin Y, Yu F et al (2015) Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). Mol Cell Proteomics 14:1009–1023CrossRefPubMedPubMedCentral

21.

Wank SA, Harkins R, Jensen RT, Shapira H, de Weerth A et al (1992) Purification, molecular cloning, and functional expression of the cholecystokinin receptor from rat pancreas. Proc Natl Acad Sci U S A 89:3125–3129CrossRefPubMedPubMedCentral

22.

Kulaksiz H, Arnold R, Goke B, Maronde E, Meyer M et al (2000) Expression and cell-specific localization of the cholecystokinin B/gastrin receptor in the human stomach. Cell Tissue Res 299:289–298CrossRefPubMed

23.

Baldwin GS, Shulkes A (2007) CCK receptors and cancer. Curr Top Med Chem 7:1232–1238CrossRefPubMed

24.

Moonka R, Zhou W, Bell RH Jr (1999) Cholecystokinin-A receptor messenger RNA expression in human pancreatic cancer. J Gastrointest Surg 3:134–140CrossRefPubMed

25.

Srivastava A, Pandey SN, Dixit M, Choudhuri G, Mittal B (2008) Cholecystokinin receptor A gene polymorphism in gallstone disease and gallbladder cancer. J Gastroenterol Hepatol 23:970–975CrossRefPubMed

26.

Faridi MS, Jaiswal MS, Goel SK (2015) Expression of CCK receptors in carcinoma gallbladder and cholelithiasis: a pilot study. J Clin Diagn Res 9:PC04–PC07PubMedPubMedCentral

27.

Kazmi HR, Chandra A, Baghel K, Singh A, Nigam J et al (2014) Differential expression of cholecystokinin A receptor in gallbladder cancer in the young and elderly suggests two subsets of the same disease? Biomed Res Int 2014:625695CrossRefPubMedPubMedCentral

28.

Allfrey VG, Faulkner R, Mirsky AE (1964) Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc Natl Acad Sci U S A 51:786–794CrossRefPubMedPubMedCentral

29.

Zhao S, Xu W, Jiang W, Yu W, Lin Y et al (2010) Regulation of cellular metabolism by protein lysine acetylation. Science 327:1000–1004CrossRefPubMedPubMedCentral

30.

Gil J, Ramirez-Torres A, Encarnacion-Guevara S (2017) Lysine acetylation and cancer: A proteomics perspective. J Proteome 150:297–309CrossRef

31.

Di Gennaro E, Bruzzese F, Caraglia M, Abruzzese A, Budillon A (2004) Acetylation of proteins as novel target for antitumor therapy: review article. Amino Acids 26:435–441CrossRefPubMed

Titel: CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma
verfasst von: W. Wu
B. Ouyang
Z. Lu
H. Liu
Y. Tan
P. Cui
Publikationsdatum: 03.05.2017
Verlag: Springer London
Erschienen in: Irish Journal of Medical Science (1971 -) / Ausgabe 4/2017
Print ISSN: 0021-1265
Elektronische ISSN: 1863-4362
DOI: https://doi.org/10.1007/s11845-017-1603-2

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CCK1 receptor is involved in the regulation of protein lysine acetylation in GBC-SD cells and gallbladder carcinoma

Abstract

Background

Aims

Methods

Results

Conclusions

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Abstract

Background

Aims

Methods

Results

Conclusions

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