nach oben

Archives of Virology

Erschienen in:

01.02.2016 | Original Article

Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin

verfasst von: Meng Li, Mengzhe Li, Hong Lin, Jingxue Wang, Yanqiu Jin, Feng Han

Erschienen in: Archives of Virology | Ausgabe 2/2016

Einloggen, um Zugang zu erhalten

Abstract

While screening for new antimicrobial agents for multidrug-resistant Salmonella enterica, the novel lytic bacteriophage STP4-a was isolated and characterized. Phage morphology revealed that STP4-a belongs to the family Myoviridae. Bacterial challenge assays showed that different serovars of Salmonella enterica were susceptible to STP4-a infection. The genomic characteristics of STP4-a, containing 159,914 bp of dsDNA with an average GC content of 36.86 %, were determined. Furthermore, the endolysin of STP4-a was expressed and characterized. The novel endolysin, LysSTP4, has hydrolytic activity towards outer-membrane-permeabilized S. enterica and Escherichia coli. These results provide essential information for the development of novel phage-based biocontrol agents against S. enterica.

Nur mit Berechtigung zugänglich

Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jones JL, Griffin PM (2011) Foodborne illness acquired in the United States—major pathogens. Emerg Infect Dis 17:7–15PubMedCentralCrossRefPubMed

O’Mahony R, Quinn T, Drudy D, Walsh C, Whyte P, Mattar S, Fanning S (2006) Antimicrobial resistance in nontyphoidal Salmonella from food sources in Colombia: evidence for an unusual plasmid-localized class 1 integron in serotypes Typhimurium and Anatum. Microb Drug Resist 12:269–277CrossRefPubMed

Garcia P, Martinez B, Obeso JM, Rodriguez A (2008) Bacteriophages and their application in food safety. Lett Appl Microbiol 47:479–485CrossRefPubMed

Lang LH (2006) FDA approves use of bacteriophages to be added to meat and poultry products. Gastroenterology 131:1370PubMed

Whichard JM, Sriranganathan N, Pierson FW (2003) Suppression of Salmonella growth by wild-type and large-plaque variants of bacteriophage Felix O1 in liquid culture and on chicken frankfurters. J Food Prot 66:220–225PubMed

Zinno P, Devirgiliis C, Ercolini D, Ongeng D, Mauriello G (2014) Bacteriophage P22 to challenge Salmonella in foods. Int J Food Microbiol 191C:69–74CrossRef

Sillankorva SM, Oliveira H, Azeredo J (2012) Bacteriophages and their role in food safety. Int J Microbiol 2012:863945PubMedCentralCrossRefPubMed

Schmelcher M, Donovan DM, Loessner MJ (2012) Bacteriophage endolysins as novel antimicrobials. Future Microbiol 7:1147–1171PubMedCentralCrossRefPubMed

Garcia P, Martinez B, Rodriguez L, Rodriguez A (2010) Synergy between the phage endolysin LysH5 and nisin to kill Staphylococcus aureus in pasteurized milk. Int J Food Microbiol 141:151–155CrossRefPubMed

10.

Zhang H, Bao H, Billington C, Hudson JA, Wang R (2012) Isolation and lytic activity of the Listeria bacteriophage endolysin LysZ5 against Listeria monocytogenes in soya milk. Food Microbiol 31:133–136CrossRefPubMed

11.

Courchesne NM, Parisien A, Lan CQ (2009) Production and application of bacteriophage and bacteriophage-encoded lysins. Recent Pat Biotechnol 3:37–45CrossRefPubMed

12.

Lim JA, Shin H, Kang DH, Ryu S (2012) Characterization of endolysin from a Salmonella Typhimurium-infecting bacteriophage SPN1S. Res Microbiol 163:233–241CrossRefPubMed

13.

Borysowski J, Weber-Dabrowska B, Gorski A (2006) Bacteriophage endolysins as a novel class of antibacterial agents. Exp Biol Med (Maywood) 231:366–377

14.

Park M, Lee JH, Shin H, Kim M, Choi J, Kang DH, Heu S, Ryu S (2012) Characterization and comparative genomic analysis of a novel bacteriophage, SFP10, simultaneously inhibiting both Salmonella enterica and Escherichia coli O157:H7. Appl Environ Microbiol 78:58–69PubMedCentralCrossRefPubMed

15.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410CrossRefPubMed

16.

Marchler-Bauer A, Anderson JB, Derbyshire MK, DeWeese-Scott C, Gonzales NR, Gwadz M, Hao L, He S, Hurwitz DI, Jackson JD, Ke Z, Krylov D, Lanczycki CJ, Liebert CA, Liu C, Lu F, Lu S, Marchler GH, Mullokandov M, Song JS, Thanki N, Yamashita RA, Yin JJ, Zhang D, Bryant SH (2007) CDD: a conserved domain database for interactive domain family analysis. Nucleic Acids Res 35:D237–D240PubMedCentralCrossRefPubMed

17.

Zdobnov EM, Apweiler R (2001) InterProScan–an integration platform for the signature-recognition methods in InterPro. Bioinformatics 17:847–848CrossRefPubMed

18.

Lowe TM, Eddy SR (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25:955–964PubMedCentralCrossRefPubMed

19.

Grant JR, Stothard P (2008) The CGView Server: a comparative genomics tool for circular genomes. Nucleic Acids Res 36:W181–W184PubMedCentralCrossRefPubMed

20.

Mahadevan P, King JF, Seto D (2009) CGUG: in silico proteome and genome parsing tool for the determination of “core” and unique genes in the analysis of genomes up to ca. 1.9 Mb. BMC Res Notes 2:168PubMedCentralCrossRefPubMed

21.

Strating H, Clarke AJ (2001) Differentiation of bacterial autolysins by zymogram analysis. Analytical biochemistry 291:149–154CrossRefPubMed

22.

Clarke AJ (1993) Extent of peptidoglycan O acetylation in the tribe Proteeae. J Bacteriol 175:4550–4553PubMedCentralPubMed

23.

Han F, Li M, Lin H, Wang J, Cao L, Khan MN (2014) The novel Shewanella putrefaciens-infecting bacteriophage Spp001: genome sequence and lytic enzymes. J Ind Microbiol Biotechnol 41:1017–1026CrossRefPubMed

24.

Mikoulinskaia GV, Odinokova IV, Zimin AA, Lysanskaya VY, Feofanov SA, Stepnaya OA (2009) Identification and characterization of the metal ion-dependent l-alanoyl-d-glutamate peptidase encoded by bacteriophage T5. FEBS J 276:7329–7342CrossRefPubMed

25.

Sillankorva S, Oliveira D, Moura A, Henriques M, Faustino A, Nicolau A, Azeredo J (2011) Efficacy of a broad host range lytic bacteriophage against E. coli adhered to urothelium. Curr Microbiol 62:1128–1132CrossRefPubMed

26.

Kuznetsov YG, Chang SC, McPherson A (2011) Investigation of bacteriophage T4 by atomic force microscopy. Bacteriophage 1:165–173PubMedCentralCrossRefPubMed

27.

Marti R, Zurfluh K, Hagens S, Pianezzi J, Klumpp J, Loessner MJ (2013) Long tail fibres of the novel broad-host-range T-even bacteriophage S16 specifically recognize Salmonella OmpC. Mol Microbiol 87:818–834CrossRefPubMed

28.

Santos SB, Kropinski AM, Ceyssens PJ, Ackermann HW, Villegas A, Lavigne R, Krylov VN, Carvalho CM, Ferreira EC, Azeredo J (2011) Genomic and proteomic characterization of the broad-host-range Salmonella phage PVP-SE1: creation of a new phage genus. J Virol 85:11265–11273PubMedCentralCrossRefPubMed

29.

Toreci K, Erdem B, Ongen B (2013) Salmonella serovars isolated in Turkey up to the end of year 2011. Mikrobiyol Bul 47:442–460CrossRefPubMed

30.

Davidson AR, Cardarelli L, Pell LG, Radford DR, Maxwell KL (2012) Long noncontractile tail machines of bacteriophages. Adv Exp Med Biol 726:115–142CrossRefPubMed

31.

Wang IN, Smith DL, Young R (2000) Holins: the protein clocks of bacteriophage infections. Annu Rev Microbiol 54:799–825CrossRefPubMed

32.

Tran TA, Struck DK, Young R (2007) The T4 RI antiholin has an N-terminal signal anchor release domain that targets it for degradation by DegP. J Bacteriol 189:7618–7625PubMedCentralCrossRefPubMed

33.

Summer EJ, Berry J, Tran TA, Niu L, Struck DK, Young R (2007) Rz/Rz1 lysis gene equivalents in phages of Gram-negative hosts. J Mol Biol 373:1098–1112CrossRefPubMed

34.

Matsushita I, Yanase H (2008) A novel thermophilic lysozyme from bacteriophage φIN93. Biochem Biophys Res Commun 377:89–92CrossRefPubMed

35.

Lavigne R, Briers Y, Hertveldt K, Robben J, Volckaert G (2004) Identification and characterization of a highly thermostable bacteriophage lysozyme. Cell Mol Life Sci 61:2753–2759CrossRefPubMed

36.

Black LM, Hogness DS (1968) The lysozyme of bacteriophage λ. J Biol Chem 244:1968–1975

37.

Catalao MJ, Gil F, Moniz-Pereira J, Sao-Jose C, Pimentel M (2013) Diversity in bacterial lysis systems: bacteriophages show the way. FEMS Microbiol Rev 37:554–571CrossRefPubMed

38.

Walmagh M, Boczkowska B, Grymonprez B, Briers Y, Drulis-Kawa Z, Lavigne R (2012) Characterization of five novel endolysins from Gram-negative infecting bacteriophages. Appl Microbiol Biotechnol 97:4369–4375CrossRefPubMed

39.

Vaara M (1992) Agents that increase the permeability of the outer membrane. Microbiol Rev 56:395–411PubMedCentralPubMed

40.

Briers Y, Walmagh M, Lavigne R (2011) Use of bacteriophage endolysin EL188 and outer membrane permeabilizers against Pseudomonas aeruginosa. J Appl Microbiol 110:778–785CrossRefPubMed

41.

Oliveira H, Thiagarajan V, Walmagh M, Sillankorva S, Lavigne R, Neves-Petersen MT, Kluskens LD, Azeredo J (2014) A thermostable Salmonella phage endolysin, Lys68, with broad bactericidal properties against gram-negative pathogens in presence of weak acids. PLoS One 9:e108376PubMedCentralCrossRefPubMed

Titel: Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin
verfasst von: Meng Li
Mengzhe Li
Hong Lin
Jingxue Wang
Yanqiu Jin
Feng Han
Publikationsdatum: 01.02.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 2/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2647-0

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Notfall-TEP der Hüfte ist auch bei 90-Jährigen machbar

26.04.2024 Hüft-TEP Nachrichten

Ob bei einer Notfalloperation nach Schenkelhalsfraktur eine Hemiarthroplastik oder eine totale Endoprothese (TEP) eingebaut wird, sollte nicht allein vom Alter der Patientinnen und Patienten abhängen. Auch über 90-Jährige können von der TEP profitieren.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

25.04.2024 Hypertonie Nachrichten

Beginnen ältere Männer im Pflegeheim eine Antihypertensiva-Therapie, dann ist die Frakturrate in den folgenden 30 Tagen mehr als verdoppelt. Besonders häufig stürzen Demenzkranke und Männer, die erstmals Blutdrucksenker nehmen. Dafür spricht eine Analyse unter US-Veteranen.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2016

Molecular characterization of coxsackievirus A21 in Shandong, China

Functional characterization of a new promoter isolated from torque teno sus virus 1

Complete genome sequence of a strain of Actinidia virus X detected in Ribes nigrum cv. Baldwin showing unusual symptoms

Complete genome sequence of currant latent virus (genus Cheravirus, family Secoviridae)

Clinical validation of a novel real-time human papillomavirus assay for simultaneous detection of 14 high-risk HPV type and genotyping HPV type 16 and 18 in China