Background
Methods
Sample collection and preparation
Semen analysis
Measurement of white blood cells
Measurement of reactive oxygen species
Statistical analysis
Results
Semen parameters across donors and patients
Parameter | Donors | Patients | P value |
---|---|---|---|
All donors vs. all patients
| |||
Volume (mL)
| 3.36 ± 2.02 | 3.15 ± 1.49 | 0.95 |
Concentration (X10
6
/mL)
| 54.26 ± 32.19 | 45.33 ± 51.00 |
0.001
|
Motility (%)
| 53.70 ± 15.00 | 46.95 ± 20.16 |
0.016
|
Endtz(X10
6
/mL)
| 1.04 ± 2.54 | 0.50 ± 1.62 | 0.41 |
Normal morphology (%)
| 6.93 ± 3.91 | 3.20 ± 2.91 |
<0.001
|
ROS (RLU/s)
| 228.80 ± 396.27 | 12540.40 ± 70846.85 |
<0.001
|
64.8(21.1,198.2)a | 267.5(59.4, 1320)a | ||
Proven donors vs. all patients
| |||
Volume (mL)
| 4.24 ± 2.13 | 3.15 ± 1.49 |
0.008
|
Concentration (X10
6
/mL)
| 60.07 ± 33.44 | 45.33 ± 51.00 |
0.002
|
Motility (%)
| 50.85 ± 13.52 | 46.95 ± 20.16 | 0.30 |
WBC X10
6
/mL)
| 0.00 ± 0.00 | 0.50 ± 1.62 | 0.09 |
Normal morphology (%)
| 7.00 ± 4.35 | 3.20 ± 2.91 |
<0.001
|
ROS (RLU/s)
| 149.46 ± 275.08 | 12540.40 ± 70846.85 |
<0.001
|
75.8(33.3, 147.8)a | 267.5(59.4, 1320)a | ||
Proven donors <2y vs. all patients
| |||
Volume (mL)
| 5.03 ± 2.22 | 3.15 ± 1.49 |
<0.001
|
Concentration (X10
6
/mL)
| 61.59 ± 23.93 | 45.33 ± 51.00 |
0.002
|
Motility (%)
| 49.88 ± 8.68 | 46.95 ± 20.16 | 0.40 |
Endtz(X10
6
/mL)
| 0.00 ± 0.00 | 0.50 ± 1.62 | 0.18 |
Normal morphology (%)
| 6.77 ± 4.95 | 3.20 ± 2.91 |
0.006
|
ROS (RLU/s)
| 54.53 ± 45.31 | 12540.40 ± 70846.85 |
<0.001
|
75.8(33.3, 147.8)a | 267.5(59.4, 1320)a |
Correlations with semen parameters
Parameter | Group | Cut-off | Sensitivity | Specificity | AUC |
---|---|---|---|---|---|
All donors + All patients
| |||||
Concentration (X10
6
/mL)
|
31.55
|
56.6
|
74.5
|
0.640
| |
Motility (%)
|
45.5
|
51.9
|
77.8
|
0.602
| |
Morphology (%)
|
3.5
|
62.9
|
78.0
|
0.775
| |
Proven donors + All patients
| |||||
Concentration (X10
6
/mL)
|
27.8
|
50.9
|
85.7
|
0.674
| |
Motility (%)
|
45.5
|
51.9
|
69.2
|
0.562
| |
Morphology (%)
|
2.5
|
50.0
|
89.5
|
0.765
| |
Proven donors < 2 years + All patients
| |||||
Concentration (X10
6
/mL)
|
28.5
|
52.5
|
100
|
0.728
| |
Motility (%)
|
46.5
|
52.5
|
75.0
|
0.562
| |
Morphology (%)
|
11.5
|
98.7
|
38.5
|
0.723
|