Background
Materials and methods
Animals
Experimental protocol
Surgical procedures for renal function studies
Experimental protocol for renal function studies
Renal cortex IL-β, IL-10, TNF-α measurements
Activity of NADPH oxidase by lucigenin-enhanced chemiluminescence
Real-time quantitative RT-PCR (qRT-PCR) analysis
Nuclear/cytosolic fractionation
Western blot analysis
Electrophoretic mobility shift assay (EMSA)
Statistical analysis
Results
Physiological parameters
Parameter | LZ Control | LZ PDTC | ZDF Control | ZDF PDTC |
---|---|---|---|---|
Food intake (g/day) | 23.4 ± 1.4 | 24.0 ± 1.7 | 37.7 ± 1.6*
| 36.7 ± 1.2*
|
Body weight (g) | 290.1 ± 6.5 | 288.6 ± 4.3 | 435.3 ± 7.3*
| 431.1 ± 7.5*
|
Blood glucose (mmol/L) | 5.5 ± 0.2 | 5.3 ± 0.3 | 8.6 ± 0.3*
| 7.0 ± 0.1*#
|
Insulin (nmol/L) | 0.63 ± 0.04 | 0.60 ± 0.04 | 4.17 ± 0.20*
| 1.67 ± 0.14*#
|
Triglycerides (mg/dl) | 61.8 ± 5.0 | 57.4 ± 4.3 | 315.9 ± 13.3*
| 132.2 ± 15.5*#
|
Heart rate (bpm) | 370.6 ± 5.9 | 367.8 ± 5.6 | 363.3 ± 4.2 | 362.4 ± 4.6 |
Effect of PDTC on renal function in ZDF rats
Group | LZ Control | LZ PDTC | ZDF Control | ZDF PDTC |
---|---|---|---|---|
Kidney weight (g) | 1.02 ± 0.03 | 1.01 ± 0.02 | 1.83 ± 0.04*
| 1.80 ± 0.03*
|
Water intake (ml/day) | 17.3 ± 0.64 | 16.9 ± 0.84 | 22.3 ± 0.69*
| 21.8 ± 0.74*
|
Creatinine (mg/dl) | 0.62 ± 0.05 | 0.63 ± 0.05 | 1.25 ± 0.13*
| 0.77 ± 0.04#
|
Urinary albumin (mg/24 h) | 10.69 ± 0.97 | 10.36 ± 1.55 | 60.49 ± 4.93*
| 36.19 ± 3.23*#
|
Urine Volume (ml/day) | 7.09 ± 0.48 | 6.83 ± 0.64 | 8.28 ± 0.50*
| 10.02 ± 0.90*#
|
Normalized urine volume (ml/day/kg · body wt) | 24.74 ± 2.17 | 24.10 ± 2.44 | 19.42 ± 1.50*
| 23.95 ± 1.21#
|
UNaV (mmol/day) | 224.5 ± 13.3 | 227.8 ± 20.4 | 264.4 ± 11.4*
| 298.9 ± 14.5*#
|
Normalized UnaV (μmol/day /kg · body wt) | 782.8 ± 62.9 | 801.8 ± 74.5 | 596.3 ± 37.8*
| 730.1 ± 58.2#
|
GFR (ml/min) | 1.01 ± 0.04 | 0.96 ± 0.05 | 0.75 ± 0.03*
| 0.88 ± 0.04#
|