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Journal of Cardiovascular Translational Research

Erschienen in:

01.06.2009

Chronic Treatment with Clenbuterol Modulates Endothelial Progenitor Cells and Circulating Factors in a Murine Model of Cardiomyopathy

verfasst von: James E. Rider, Sean P. Polster, Sangjin Lee, Nathan J. Charles, Neeta Adhikari, Ami Mariash, George Tadros, Jenna Stangland, Ryszard T. Smolenski, Cesare M Terracciano, Paul J.R. Barton, Emma J. Birks, Magdi H. Yacoub, Leslie W. Miller, Jennifer L. Hall

Erschienen in: Journal of Cardiovascular Translational Research | Ausgabe 2/2009

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Abstract

The purpose of this study was to determine the effects of chronic treatment with the beta 2 adrenergic receptor agonist clenbuterol on endothelial progenitor cells (EPC) in a well-characterized model of heart failure, the muscle LIM protein knockout (MLP^−/−) mouse. MLP^−/− mice were treated daily with clenbuterol (2 mg/kg) or saline subcutaneously for 6 weeks. Clenbuterol led to a 30% increase in CD31⁺ cells in the bone marrow of MLP^−/− heart failure mice (p < 0.004). Clenbuterol did not improve ejection fraction. Clenbuterol treatment in MLP^−/− mice was associated with significant changes in the following circulating factors: tissue inhibitor of metalloproteinase-type 1, leukemia inhibitory factor 1, C-reactive protein, apolipoprotein A1, fibroblast growth factor 2, serum glutamic oxaloacetic transaminase, macrophage-derived chemokine, and monocyte chemoattractant protein-3. Clenbuterol treatment in the MLP^−/− model of heart failure did not rescue heart function, yet did increase CD31⁺ cells in the bone marrow. This is the first evidence that a beta 2 agonist increases EPC proliferation in the bone marrow in a preclinical model of heart failure.

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Titel: Chronic Treatment with Clenbuterol Modulates Endothelial Progenitor Cells and Circulating Factors in a Murine Model of Cardiomyopathy
verfasst von: James E. Rider
Sean P. Polster
Sangjin Lee
Nathan J. Charles
Neeta Adhikari
Ami Mariash
George Tadros
Jenna Stangland
Ryszard T. Smolenski
Cesare M Terracciano
Paul J.R. Barton
Emma J. Birks
Magdi H. Yacoub
Leslie W. Miller
Jennifer L. Hall
Publikationsdatum: 01.06.2009
Verlag: Springer US
Erschienen in: Journal of Cardiovascular Translational Research / Ausgabe 2/2009
Print ISSN: 1937-5387
Elektronische ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-009-9089-6

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Chronic Treatment with Clenbuterol Modulates Endothelial Progenitor Cells and Circulating Factors in a Murine Model of Cardiomyopathy

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