Skip to main content
main-content

01.12.2017 | Research article | Ausgabe 1/2017 Open Access

BMC Cancer 1/2017

Clinical significance of PCDH10 promoter methylation in diffuse large B-cell lymphoma

Zeitschrift:
BMC Cancer > Ausgabe 1/2017
Autoren:
Wenting Huang, Xuemin Xue, Ling Shan, Tian Qiu, Lei Guo, Jianming Ying, Ning Lu
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12885-017-3810-7) contains supplementary material, which is available to authorized users.
Wenting Huang and Xuemin Xue are co-first authors
Jianming Ying and Ning Lv are co-senior authors

Abstract

Background

PCDH10, one of the non-clustered protocadherins, is identified as a tumor suppressor gene in many tumors. Recently, promoter methylation of PCDH10 was found in diffuse large B-cell lymphoma (DLBCL) but not in normal lymph nodes, suggesting that its epigenetic aberrance is essential to the lymphomagenesis. However, there are few studies on the clinicopathological relevance and prognostic significance of PCDH10 methylation status in DLBCL.

Methods

One hundred-seven cases of DLBCL between Jan 2009 and Jul 2010 were selected to extract genomic DNA and perform bisulfite modification. Their methylation status of PCDH10 promoter were accessed by methylation-specific PCR (MSP) with methylated and unmethylated primers. Analysis of overall survival and clinicopathological correlation were conducted.

Results

PCDH10 hypermethylation were found in 54.2% (58/107) of DLBCL cases, but only 12.5% (1/8) in reactive lymph node/follicular hyperplasia. In RCHOP-treated cohort, promoter methylation of PCDH10 is an independent prognostic indicator of worse overall survival (p = 0.017; HR 4.045; 95%CI 1.287–12.711) and worse progress-free survival (p = 0.014; HR 2.977; 95%CI 1.245–7.119). Whereas, PCDH10 hypermethylation wasn’t correlated with MYC translocation and cell of origin classification using Hans model.

Conclusions

PCDH10 methylation status could serve as a valuable biomarker for risk classification, and a potential therapeutic target for demethylating drugs in DLBCL in the future.
Zusatzmaterial
Additional file 1: Table S1. The clinicopathological characteristics and PCDH10 methylation status of 107 cases are showed in this table. (XLS 32 kb)
12885_2017_3810_MOESM1_ESM.xls
Additional file 2: Table S2. The details of survival (OS and PFS) and other clinicopathological characteristics of 65 RCHOP-treated cases are showed in this table. (XLS 40 kb)
12885_2017_3810_MOESM2_ESM.xls
Additional file 3: Diagram S1. Description: The location of the primers in relation to the PCDH10 promoter and start site. (PPTX 194 kb)
12885_2017_3810_MOESM3_ESM.pptx
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2017

BMC Cancer 1/2017 Zur Ausgabe

Neu im Fachgebiet Onkologie

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Onkologie und bleiben Sie gut informiert – ganz bequem per eMail.

Bildnachweise