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Journal of Cancer Research and Clinical Oncology

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01.12.2015 | Original Article – Cancer Research

Clinicopathological significance of cancer stem-like cell markers in high-grade neuroendocrine carcinoma of the lung

verfasst von: Masahiro Morise, Tomoyuki Hishida, Akiko Takahashi, Junji Yoshida, Yuichiro Ohe, Kanji Nagai, Genichiro Ishii

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 12/2015

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Abstract

Purpose

This study aimed to evaluate the clinicopathological significance of cancer stem-like cell (CSLC) markers in high-grade neuroendocrine carcinoma (HGNEC) of the lung, including small cell lung carcinoma (SCLC) and large cell neuroendocrine carcinoma (LCNEC).

Methods

We retrospectively studied patients who underwent surgical resection of SCLC (n = 60) and LCNEC (n = 45) to analyze their clinicopathological profiles and the immunohistochemical expression of putative CSLC markers (Caveolin, Notch, CD44, CD166, SOX2, ALDH1, and Musashi1). Staining scores for these markers in tumor cells were calculated by multiplying the percentage of positive tumor cells per lesion by the staining intensity level (0, 1, and 2); a score of ≥10 represented positive expression.

Results

There was a difference between SCLC and LCNEC with respect to both SOX2 (55 vs. 27 %, p = 0.003) and CD166 (27 vs. 47 %, p = 0.034) expression. ALDH1 expression was equally observed in SCLC and LCNEC (67 vs. 73 %, p = 0.46), and patients with ALDH1-positive HGNEC had significantly worse recurrence-free survival (RFS) and overall survival (OS) rates than those with ALDH1-negative HGNEC (5-year RFS: 39 vs. 67 %, p = 0.009; 5-year OS: 50 vs. 79 %, p = 0.021). A multivariate analysis revealed that positive ALDH1 expression was an independent unfavorable prognostic factor with respect to both RFS and OS.

Conclusions

The differences in the expression profiles of CSLC markers might reflect morphological differences between SCLC and LCNEC. Positive ALDH1 expression in lung HGNEC was associated with an unfavorable patient prognosis, which suggested that ALDH1-positive tumor cells might be future therapeutic targets for the treatment of lung HGNEC.

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Titel: Clinicopathological significance of cancer stem-like cell markers in high-grade neuroendocrine carcinoma of the lung
verfasst von: Masahiro Morise
Tomoyuki Hishida
Akiko Takahashi
Junji Yoshida
Yuichiro Ohe
Kanji Nagai
Genichiro Ishii
Publikationsdatum: 01.12.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 12/2015
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-015-1985-3

Springer Medizin

Clinicopathological significance of cancer stem-like cell markers in high-grade neuroendocrine carcinoma of the lung