Background
Material and methods
Patient material
Circulating tumor cell (CTC) detection
Whole genome amplification (WGA) and quality control of CTCs
DNA extraction from tumor samples
Next-generation sequencing
ARID1A immunohistochemical analysis
Statistical analyses
Results
CTC detection in blood samples from BCBM patients
≥ 5 CTCs (EpCAM dependent) | ≥ 1 CTC (EpCAM independent) | |||||||
---|---|---|---|---|---|---|---|---|
Total (n) | Neg (n) | Pos (n) | p value | Total (n) | Neg (n) | Pos (n) | p value | |
Total | 44 | 35 | 9 | 46 | 31 | 15 | ||
Histology | ||||||||
Ductal | 24 | 20 | 4 | 0.655 | 25 | 17 | 8 | 0.552 |
Lobular | 4 | 3 | 1 | 5 | 3 | 2 | ||
Others | 2 | 1 | 1 | 3 | 1 | 2 | ||
n.a. | 14 | 13 | ||||||
Age at BM | ||||||||
< Mean | 21 | 15 | 6 | 0.213 | 25 | 18 | 7 | 0.476 |
> Mean | 23 | 20 | 3 | 21 | 13 | 8 | ||
Hormone | ||||||||
Neg | 21 | 17 | 4 | 0.878 | 20 | 9 | 11 | 0.018 |
Receptor | ||||||||
Pos | 19 | 15 | 4 | 21 | 17 | 4 | ||
n.a. | 4 | 5 | ||||||
ERBB2 | ||||||||
Neg | 16 | 10 | 6 | 0.029 | 15 | 6 | 9 | 0.021 |
Pos | 24 | 22 | 2 | 26 | 20 | 6 | ||
n.a. | 4 | 5 | ||||||
Subtype | ||||||||
HR-pos | 8 | 5 | 3 | 0.102 | 5 | 3 | 2 | 0.044 |
TNBC | 8 | 5 | 3 | 10 | 3 | 7 | ||
ERBB2-pos | 24 | 22 | 2 | 26 | 20 | 6 | ||
n.a. | 4 | 5 | ||||||
Brain surgery | ||||||||
No OP | 14 | 10 | 4 | 0.388 | 15 | 9 | 6 | 0.471 |
OP | 30 | 25 | 5 | 20 | 22 | 9 | ||
n.a. | 0 | 11 | ||||||
Oligo brain met. | ||||||||
Multiple | 30 | 24 | 6 | 0.827 | 28 | 16 | 12 | 0.080 |
Oligo | 13 | 10 | 3 | 17 | 14 | 3 | ||
n.a. | 1 | 1 | ||||||
Bone met. | ||||||||
No | 25 | 23 | 2 | 0.021 | 25 | 18 | 7 | 0.476 |
Yes | 19 | 12 | 7 | 21 | 13 | 8 | ||
Liver met. | ||||||||
No | 33 | 28 | 5 | 0.165 | 31 | 22 | 9 | 0.471 |
Yes | 11 | 7 | 4 | 15 | 9 | 6 | ||
Pulmonary met. | ||||||||
No | 36 | 29 | 7 | 0.743 | 38 | 28 | 10 | 0.064 |
Yes | 8 | 6 | 2 | 8 | 3 | 5 |
Clinical value of detected CTCs
Bone met. | Liver met. | Pulmonary met. | |
---|---|---|---|
HR-pos | 7 | 2 | 2 |
ERBB2-pos | 3 | 3 | 2 |
TNBC | 14 | 10 | 5 |