Background
Methods
Animals
Drug treatment
Estradiol (E2), Luteinising Hormone (LH) and Follicle-Stimulating Hormone (FSH) assays
Observation of oestrous cyclicity
Histological study and follicle counting
Statistical analysis
Results
Combination pretreatment completely prevented gonadotropin flare-up
Groups | n | FSH (mIU/ml) | LH (mIU/ml) | E2 (pg/ml) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1st day | 3rd day | 5th day | 8th day | 1st day | 3rd day | 5th day | 8th day | 1st day | 3rd day | 5th day | 8th day | ||
Control
| 12 | 2.45±0.13 | 2.43±0.08 | 2.32±0.06 | 2.45±0.12 | 2.77±0.18 | 2.83±0.19 | 2.84±0.11 | 2.90±0.21 | 252.98±10.29 | 242.00±12.96 | 256.60±11.75 | 246.03±2.80 |
CDDP
| 12 | 2.51±0.16 | 2.49±0.09 | 2.41±0.05 | 2.44±0.14 | 2.75±0.16 | 2.83±0.20 | 2.66±0.32 | 2.89±0.17 | 257.71±13.28 | 252.60±13.90 | 250.59±11.07 | 250.98±4.09 |
GnRHa
| 12 | 2.47±0.08 | 2.71±0.10 | 2.21±0.07 | 2.33±0.11 | 2.87±0.18 | 3.93±0.13a | 2.81±0.27 | 2.89±0.11 | 250.34±13.01 | 265.42±13.49 | 197.10±13.43 | 159.63±2.52ab |
GnRHant
| 12 | 2.33±0.07 | 2.22±0.11 | 2.01±0.07 | 2.14±0.14 | 2.70±0.12 | 2.56±0.06 | 2.42±0.14 | 2.62±0.08 | 252.83±11.75 | 153.04±12.37a | 179.87±13.83 | 151.87±3.43ab |
Combination (lng)
| 12 | 2.52±0.13 | 2.29±0.10 | 2.44±0.05 | 2.48±0.07 | 2.96±0.20 | 2.74±0.05 | 2.73±0.13 | 2.64±0.30 | 247.65±2.80 | 171.95±7.20a | 185.10±13.86 | 96.57±5.79a |
Combination (sht)
| 12 | 2.64±0.09 | 2.33±0.10 | 2.24±0.25 | 2.30±0.16 | 2.88±0.12 | 2.53±0.17 | 2.86±0.21 | 2.75±0.07 | 260.86±19.57 | 163.60±17.4a | 214.63±11.77 | 142.21±8.34ab |
Combination pretreatment partly restored oestrous cyclicity
Combination pretreatment preserved more primordial follicles
Groups | n | Primordial follicle | Growing follicle | Mature follicle | Total |
---|---|---|---|---|---|
Control
| 12 | 370.5±6.9 | 235.3±31.1 | 54.6±17.1 | 660±24.4 |
CDDP
| 12 | 136.5±23.1a | 28.6±4.7a | 9.1±6.3a | 174.2±32.5a |
GnRHa
| 12 | 213.2±22.3abc | 41.6±12.3a | 13±10.6a | 267.8±34.2abc |
GnRHant
| 12 | 227.5±8.9abc | 71.5±12.0ab | 19.5±6.8ab | 318.5±6.9abc |