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International Urogynecology Journal
Erschienen in: International Urogynecology Journal 10/2008

01.10.2008 | Original Article

Combined estrogen and ghrelin administration decreases expression of p27kip1 and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats

verfasst von: Diaa E. E. Rizk, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Mohammed Shafiullah, Mohamed A. Fahim

Erschienen in: International Urogynecology Journal | Ausgabe 10/2008

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Abstract

We compared estrogen and/or ghrelin effects on pelvic floor muscles in old versus young adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n = 24 × 2) received 42 daily intraperitoneal 17-β estradiol (10 μg kg−1), ghrelin (2 μg kg−1), both, or vehicle (n = 6 × 4/group). Cytoplasmic p27kip1 expression and isomyosin I proportion in striated urethral and anal sphincters and levator ani were measured, respectively, by Western blot analysis and gel electrophoresis with immunohistochemistry of muscle ghrelin receptors and radioimmunoassay of circulating growth hormone. In young adult rats, estrogen significantly decreased cytoplasmic p27kip1 and isomyosin I signal intensities. In old rats, ghrelin and estrogen/ghrelin significantly decreased both intensities with greater estrogen/ghrelin effect. Ghrelin receptors were not immunostained in any muscle. Estrogen and/or ghrelin significantly increased or decreased, respectively, circulating growth hormone in old and young adult rats. Estrogen/ghrelin administration reversed pelvic floor muscle ageing changes in old ovariectomized rats through growth hormone production.
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Metadaten
Titel
Combined estrogen and ghrelin administration decreases expression of p27kip1 and proportion of isomyosin type I in the striated urethral and anal sphincters and levator ani of old ovariectomized rats
verfasst von
Diaa E. E. Rizk
Hazem A. Hassan
Ahmed H. Al-Marzouqi
Mohammed Shafiullah
Mohamed A. Fahim
Publikationsdatum
01.10.2008
Verlag
Springer-Verlag
Erschienen in
International Urogynecology Journal / Ausgabe 10/2008
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-008-0638-3

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