Erschienen in:
17.01.2019 | Annotated Sequence Record
Complete genome sequence of a novel negevirus isolated from Culex tritaeniorhynchus in China
verfasst von:
Yuanyuan Wang, Xiaofang Guo, Honghong Peng, Yunlan Lu, Xucan Zeng, Ke Dai, Shuqing Zuo, Hongning Zhou, Jiusong Zhang, Yigang Tong
Erschienen in:
Archives of Virology
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Ausgabe 3/2019
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Abstract
A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus.