Erschienen in:
01.09.2011 | Annotated Sequence Record
Complete nucleotide sequences of two dsRNAs associated with a new partitivirus infecting Aspergillus fumigatus
verfasst von:
Muhammad Faraz Bhatti, Elaine M. Bignell, Robert H. A. Coutts
Erschienen in:
Archives of Virology
|
Ausgabe 9/2011
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Excerpt
Viruses are ubiquitous in all major groups of filamentous fungi, and those with RNA genomes are now classified into 10 families, of which four include double-stranded RNA (dsRNA) viruses and the remaining six include single-stranded (ss) RNA viruses [
10]. Most mycoviruses cause cryptic infections, but some cause phenotypic alterations including hypovirulence and debilitation [
10]. In the genus
Aspergillus, dsRNA mycoviruses are common in several asexual species, where they vary widely both in number and composition, but are rare in sexual
Aspergilli [
6,
13,
14]. Documentation of
Aspergillus mycoviruses concerns cataloguing the presence or absence of dsRNA elements in 0-13% of the species examined [
13], and until recently, the molecular characterisation of
Aspergillus mycoviruses was restricted to a partitivirus found in
A. ochraceus [
9]. However, in a recent screen of 366
A. fumigatus (Nakazawa) isolates, in contrast to a previous study [
14], dsRNA elements were discovered in 6.6% of the isolates, including a chrysovirus named Aspergillus fumigatus chrysovirus, the complete genome of which was cloned, sequenced and analysed [
7]. We now report that the dsRNA profile found in another group of virus-infected
A. fumigatus isolates parallels that found in the genus
Partitivirus [
5], and the complete bipartite genome of one strain has been cDNA cloned, sequenced and analysed. Phylogenetic analysis was performed to determine its evolutionary relationship status with other members of the family
Partitiviridae. …