Erschienen in:
01.09.2011 | Brief Report
Usefulness of a genotypic resistance test using dried blood spot specimens in African HIV-infected children with virological failure according to the 2010-revised WHO criteria
verfasst von:
Charlotte Charpentier, Jean-Chrysostome Gody, Pascaline Tisserand, Mathieu Matta, Julien Fournier, Olivia Mbitikon, Laurent Bélec
Erschienen in:
Archives of Virology
|
Ausgabe 9/2011
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Abstract
We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All children displayed virological failure (HIV-1 RNA >3.70 log10copies/ml). Testing for resistance genotype was carried out in a reference laboratory in Paris, France. A successful test result was obtained in 54 (100%) plasmas and 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to a high concordance rate of 98.1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity.