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Archives of Virology

Erschienen in:

01.06.2015 | Original Article

Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth

verfasst von: Zhiqiang Zhang, Wencheng Lin, Xiaoqi Li, Hong Cao, Yongqiang Wang, Shijun J. Zheng

Erschienen in: Archives of Virology | Ausgabe 6/2015

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Abstract

Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. It is well established that the ARV sigma-C protein induces apoptosis in host cells. However, the underlying molecular mechanism of this induction is still unclear. We report here the identification of eukaryotic elongation factor 1 alpha 1 (EEF1A1) as the interacting partner of σC. We found that σC-induced apoptosis in DF-1 cells could be completely abolished by knockdown of EEF1A1 by siRNA. Furthermore, knockdown of EEF1A1 markedly reduced ARV-induced apoptosis associated with decreased caspase-9 and -3 activation and cytochrome C release, leading to increased ARV growth in host cells. Thus, EEF1A1 plays a critical role in σC-induced apoptosis and inhibition of viral growth.

Varela R, Martinez-Costas J, Mallo M, Benavente J (1996) Intracellular posttranslational modifications of S1133 avian reovirus proteins. J Virol 70(5):2974–2981PubMedCentralPubMed

Labrada L, Bodelon G, Vinuela J, Benavente J (2002) Avian reoviruses cause apoptosis in cultured cells: viral uncoating, but not viral gene expression, is required for apoptosis induction. J Virol 76(16):7932–7941PubMedCentralPubMedCrossRef

Benavente J, Martinez-Costas J (2007) Avian reovirus: structure and biology. Virus Res 123(2):105–119PubMedCrossRef

Varela R, Benavente J (1994) Protein coding assignment of avian reovirus strain S1133. J Virol 68(10):6775–6777PubMedCentralPubMed

Schnitzer TJ, Ramos T, Gouvea V (1982) Avian reovirus polypeptides: analysis of intracellular virus-specified products, virions, top component, and cores. J Virol 43(3):1006–1014PubMedCentralPubMed

Bodelon G, Labrada L, Martinez-Costas J, Benavente J (2001) The avian reovirus genome segment S1 is a functionally tricistronic gene that expresses one structural and two nonstructural proteins in infected cells. Virology 290(2):181–191PubMedCrossRef

Shmulevitz M, Yameen Z, Dawe S et al (2002) Sequential partially overlapping gene arrangement in the tricistronic S1 genome segments of avian reovirus and Nelson Bay reovirus: implications for translation initiation. J Virol 76(2):609–618PubMedCentralPubMedCrossRef

Clarke P, Tyler KL (2003) Reovirus-induced apoptosis: a minireview. Apoptosis 8(2):141–150PubMedCrossRef

Coffey CM, Sheh A, Kim IS, Chandran K, Nibert ML, Parker JS (2006) Reovirus outer capsid protein micro1 induces apoptosis and associates with lipid droplets, endoplasmic reticulum, and mitochondria. J Virol 80(17):8422–8438PubMedCentralPubMedCrossRef

10.

Shih WL, Hsu HW, Liao MH, Lee LH, Liu HJ (2004) Avian reovirus sigmaC protein induces apoptosis in cultured cells. Virology 321(1):65–74PubMedCrossRef

11.

Barry C, Duncan R (2009) Multifaceted sequence-dependent and -independent roles for reovirus FAST protein cytoplasmic tails in fusion pore formation and syncytiogenesis. J Virol 83(23):12185–12195PubMedCentralPubMedCrossRef

12.

Top D, Barry C, Racine T, Ellis CL, Duncan R (2009) Enhanced fusion pore expansion mediated by the trans-acting Endodomain of the reovirus FAST proteins. PLoS Pathog 5(3):e1000331PubMedCentralPubMedCrossRef

13.

Salsman J, Top D, Boutilier J, Duncan R (2005) Extensive syncytium formation mediated by the reovirus FAST proteins triggers apoptosis-induced membrane instability. J Virol 79(13):8090–8100PubMedCentralPubMedCrossRef

14.

Shmulevitz M, Corcoran J, Salsman J, Duncan R (2004) Cell-cell fusion induced by the avian reovirus membrane fusion protein is regulated by protein degradation. J Virol 78(11):5996–6004PubMedCentralPubMedCrossRef

15.

Lin PY, Liu HJ, Liao MH et al (2010) Activation of PI 3-kinase/Akt/NF-kappaB and Stat3 signaling by avian reovirus S1133 in the early stages of infection results in an inflammatory response and delayed apoptosis. Virology 400(1):104–114PubMedCrossRef

16.

Connolly JL, Rodgers SE, Clarke P et al (2000) Reovirus-induced apoptosis requires activation of transcription factor NF-kappaB. J Virol 74(7):2981–2989PubMedCentralPubMedCrossRef

17.

Lin PY, Lee JW, Liao MH et al (2009) Modulation of p53 by mitogen-activated protein kinase pathways and protein kinase C delta during avian reovirus S1133-induced apoptosis. Virology 385(2):323–334PubMedCrossRef

18.

Liu HJ, Lin PY, Lee JW, Hsu HY, Shih WL (2005) Retardation of cell growth by avian reovirus p17 through the activation of p53 pathway. Biochem Biophys Res Commun 336(2):709–715PubMedCrossRef

19.

Chulu JL, Lee LH, Lee YC et al (2007) Apoptosis induction by avian reovirus through p53 and mitochondria-mediated pathway. Biochem Biophys Res Commun 356(3):529–535PubMedCrossRef

20.

Geng H, Zhang Y, Liu-Partanen Y et al (2009) Apoptosis induced by duck reovirus p10.8 protein in primary duck embryonated fibroblast and Vero E6 cells. Avian Dis 53(3):434–440PubMedCrossRef

21.

Lin PY, Liu HJ, Chang CD et al (2011) Avian reovirus S1133-induced DNA damage signaling and subsequent apoptosis in cultured cells and in chickens. Arch Virol 156(11):1917–1929PubMedCrossRef

22.

Ping-Yuan L, Hung-Jen L, Meng-Jiun L et al (2006) Avian reovirus activates a novel proapoptotic signal by linking Src to p53. Apoptosis 11(12):2179–2193PubMedCrossRef

23.

Uma R, Kataria M, Rahul S, Kataria JM (2013) Anti-neoplastic effect of avian reovirus sigma C protein on Rous sarcoma virus-induced tumors in chicken. Acta Virol 57(1):69–74PubMedCrossRef

24.

Reed LJ, Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Epidemiol 27:493–497

25.

Chen E, Proestou G, Bourbeau D, Wang E (2000) Rapid up-regulation of peptide elongation factor EF-1alpha protein levels is an immediate early event during oxidative stress-induced apoptosis. Exp Cell Res 259(1):140–148PubMedCrossRef

26.

Duttaroy A, Bourbeau D, Wang XL, Wang E (1998) Apoptosis rate can be accelerated or decelerated by overexpression or reduction of the level of elongation factor-1 alpha. Exp Cell Res 238(1):168–176PubMedCrossRef

27.

Talapatra S, Wagner JD, Thompson CB (2002) Elongation factor-1 alpha is a selective regulator of growth factor withdrawal and ER stress-induced apoptosis. Cell Death Differ 9(8):856–861PubMedCrossRef

28.

Mateyak MK, Kinzy TG (2010) eEF1A: thinking outside the ribosome. J Biol Chem 258(28):21209–21213CrossRef

29.

Li Z, Wang Y, Xue Y, Li X, Cao H, Zheng SJ (2012) Critical role for voltage-dependent anion channel 2 in infectious bursal disease virus-induced apoptosis in host cells via interaction with VP5. J Virol 86(3):1328–1338PubMedCentralPubMedCrossRef

30.

Wei L, Hou L, Zhu S, Wang J, Zhou J, Liu J (2011) Infectious bursal disease virus activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by interaction of VP5 protein with the p85alpha subunit of PI3K. Virology 417(1):211–220PubMedCrossRef

31.

Rodriguez-Grille J, Busch LK, Martinez-Costas J, Benavente J (2014) Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein. Virology 462–463:49–59PubMedCrossRef

32.

Hsu HW, Su HY, Huang PH, Lee BL, Liu HJ (2005) Sequence and phylogenetic analysis of P10- and P17-encoding genes of avian reovirus. Avian Dis 49(1):36–42PubMedCrossRef

33.

Liu HJ, Huang PH (2001) Sequence and phylogenetic analysis of the sigmaA-encoding gene of avian reovirus. J Virol Methods 98(2):99–107PubMedCrossRef

34.

Liu HJ, Lee LH, Hsu HW, Kuo LC, Liao MH (2003) Molecular evolution of avian reovirus: evidence for genetic diversity and reassortment of the S-class genome segments and multiple cocirculating lineages. Virology 314(1):336–349PubMedCrossRef

35.

Su YP, Su BS, Shien JH, Liu HJ, Lee LH (2006) The sequence and phylogenetic analysis of avian reovirus genome segments M1, M2, and M3 encoding the minor core protein muA, the major outer capsid protein muB, and the nonstructural protein muNS. J Virol Methods 133(2):146–157PubMedCrossRef

36.

Gouvea VS, Schnitzer TJ (1982) Polymorphism of the genomic RNAs among the avian reoviruses. J Gen Virol 61(Pt l):87–91

37.

Gouvea VS, Schnitzer TJ (1982) Polymorphism of the migration of double-stranded RNA genome segments of avian reoviruses. J Virol 43(2):465–471PubMedCentralPubMed

38.

Hsu CJ, Wang CY, Lee LH et al (2006) Development and characterization of monoclonal antibodies against avian reovirus sigma C protein and their application in detection of avian reovirus isolates. Avian Pathol 35(4):320–326PubMedCrossRef

39.

Lin HY, Chuang ST, Chen YT, Shih WL, Chang CD, Liu HJ (2007) Avian reovirus-induced apoptosis related to tissue injury. Avian Pathol 36(2):155–159PubMedCrossRef

Titel: Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth
verfasst von: Zhiqiang Zhang
Wencheng Lin
Xiaoqi Li
Hong Cao
Yongqiang Wang
Shijun J. Zheng
Publikationsdatum: 01.06.2015
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 6/2015
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2403-5

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