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02.03.2019 | Laboratory Investigation

Cytometric analysis of cell suspension generated by cavitron ultrasonic surgical aspirator in pediatric brain tumors

verfasst von: Martina Vaskova, Michal Tichy, Josef Zamecnik, Petr Liby, Daniela Kuzilkova, Ales Vicha, Jan Hrabeta, Tomas Kalina, Jan Stary, Ondrej Hrusak

Erschienen in: Journal of Neuro-Oncology | Ausgabe 1/2019

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Abstract

Purpose

The aim of this study was to test the possibility of using specimens obtained by a cavitron ultrasonic surgical aspirator (CUSA) in flow and mass cytometry investigations of pediatric brain tumors.

Methods

CUSA specimens obtained from 19 pediatric patients with brain tumors were investigated. Flow and mass cytometry methods were applied to analyze the composition of material collected using the CUSA. Cell suspensions were prepared from CUSA aspirates. Then sample viability was assessed by conventional flow cytometry and subsequently stained with a panel of 31 metal-labeled antibodies.

Results

Viability assessment was performed using conventional flow cytometry. Viability of cells in the acquired samples was below 50% in 16 of 19 cases. A mass cytometry investigation and subsequent analysis enabled us to discriminate brain tumor cells from contaminating leukocytes, whose proportions varied across the specimens. The addition of the viability marker cisplatin directly into the mass cytometry panel gave the means to selecting viable cells only for subsequent analyses. The proportion of non-viable cells was higher among tumor cells compared leukocytes.

Conclusions

When the analysis of the tumor cell immunophenotype is performed with markers for determining viability, the expression of the investigated markers can be evaluated. Suitable markers can be selected by high-throughput methods, such as mass cytometry, and those that are diagnostically relevant can be investigated using flow cytometry, which is more flexible in terms of time.

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Titel: Cytometric analysis of cell suspension generated by cavitron ultrasonic surgical aspirator in pediatric brain tumors
verfasst von: Martina Vaskova
Michal Tichy
Josef Zamecnik
Petr Liby
Daniela Kuzilkova
Ales Vicha
Jan Hrabeta
Tomas Kalina
Jan Stary
Ondrej Hrusak
Publikationsdatum: 02.03.2019
Verlag: Springer US
Erschienen in: Journal of Neuro-Oncology / Ausgabe 1/2019
Print ISSN: 0167-594X
Elektronische ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-019-03135-w

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Abstract

Purpose

Methods

Results

Conclusions

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