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01.07.2011 | Brief Report

Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR

verfasst von: J. Araujo, A. Pereira, M. S. Nardi, D. A. Henriques, D. A. Lautenschalager, L. M. Dutra, T. L. Ometto, R. F. Hurtado, F. Maués, A. Nava, F. A. Morais, C. C. Aires, S. Favorito, E. L. Durigon

Erschienen in: Archives of Virology | Ausgabe 7/2011

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Abstract

Current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. Specific primers to detect the presence of viral genomes were developed using an SYBR-Green-based real-time RT-PCR protocol. One hundred sixty-four rodents native to the Atlantic Forest biome were captured in São Paulo State, Brazil, and their tissues were tested. The presence of hantavirus RNA was detected in sixteen rodents: three specimens of Akodon montensis, three of Akodon cursor, two of Necromys lasiurus, one of Juliomys sp., one of Thaptomys nigrita, five of Oligoryzomys nigripes, and one of Oryzomys sp. This SYBR Green real-time RT-PCR method for detection of hantavirus may be useful for surveying hantaviruses in Brazil.

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Titel: Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR
verfasst von: J. Araujo
A. Pereira
M. S. Nardi
D. A. Henriques
D. A. Lautenschalager
L. M. Dutra
T. L. Ometto
R. F. Hurtado
F. Maués
A. Nava
F. A. Morais
C. C. Aires
S. Favorito
E. L. Durigon
Publikationsdatum: 01.07.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-0968-1

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Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR

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