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Archives of Virology

Erschienen in:

01.08.2011 | Original Article

Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay

verfasst von: Jin Yang, Mei-xin Fang, Jie Li, Guo-qiang Lou, Hang-jun Lu, Nan-ping Wu

Erschienen in: Archives of Virology | Ausgabe 8/2011

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Abstract

An improved, sensitive, specific, and rapid one-step reverse transcription loop-mediated isothermal amplification (LAMP) assay targeting the 5′ untranslated region (UTR) was developed to detect hepatitis C virus (HCV) infection. Based on an accelerating primer (AP), the present assay, named AP-LAMP, has the advantages of rapidity and sensitivity over the routine LAMP method. The possible AP-based amplification pathway during the reaction was revealed by restriction enzyme digestion and eletrophoresis. The detection limit of the AP-LAMP assay was approximately 84 IU/ml, and no cross-detection was observed. The assay was evaluated further with 126 clinical specimens, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for detection of HCV RNA.

(2002) National Institutes of Health Consensus Development Conference Statement: management of hepatitis C: 2002 June 10–12, 2002. Hepatology 36:S3–S20

Aoi Y, Hosogai M, Tsuneda S (2006) Real-time quantitative LAMP (loop-mediated isothermal amplification of DNA) as a simple method for monitoring ammonia-oxidizing bacteria. J Biotechnol 125:484–491PubMedCrossRef

Ascione A, Tartaglione T, Di Costanzo GG (2007) Natural history of chronic hepatitis C virus infection. Dig Liver Dis 39(Suppl 1):S4–S7PubMedCrossRef

Bashiardes S, Richter J, Christodoulou CG (2008) An in-house method for the detection and quantification of HCV in serum samples using a TaqMan assay real time PCR approach. Clin Chem Lab Med 46:1729–1731PubMedCrossRef

Castelain S, Descamps V, Thibault V, Francois C, Bonte D, Morel V, Izopet J, Capron D, Zawadzki P, Duverlie G (2004) TaqMan amplification system with an internal positive control for HCV RNA quantitation. J Clin Virol 31:227–234PubMedCrossRef

Crignis ED, Re MC, Cimatti L, Zecchi L, Gibellini D (2009) HIV-1 and HCV detection in dried blood spots by SYBR Green multiplex real time RT-PCR. J Virol Methods 165:51–56CrossRef

Curtis KA, Rudolph DL, Owen SM (2009) Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1. J Med Virol 81:966–972PubMedCrossRef

Daniel HD, Grant PR, Garson JA, Tedder RS, Chandy GM, Abraham P (2008) Quantitation of hepatitis C virus using an in-house real-time reverse transcriptase polymerase chain reaction in plasma samples. Diagn Microbiol Infect Dis 61:415–420PubMedCrossRef

Drexler JF, Kupfer B, Petersen N, Grotto RM, Rodrigues SM, Grywna K, Panning M, Annan A, Silva GF, Douglas J, Koay ES, Smuts H, Netto EM, Simmonds P, Pardini MI, Roth WK, Drosten C (2009) A novel diagnostic target in the hepatitis C virus genome. PLoS Med 6:e31PubMedCrossRef

10.

Fang X, Liu Y, Kong J, Jiang X (2010) Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens. Anal Chem 82:3002–3006PubMedCrossRef

11.

Glynn SA, Wright DJ, Kleinman SH, Hirschkorn D, Tu Y, Heldebrant C, Smith R, Giachetti C, Gallarda J, Busch MP (2005) Dynamics of viremia in early hepatitis C virus infection. Transfusion 45:994–1002PubMedCrossRef

12.

Gonzalez-Perez I, Vina-Rodriguez A, Cayarga AA, Rosa IG, Gonzalez YJ (2003) Design of an antisense reverse-transcriptase-polymerase chain reaction primer efficient for all hepatitis C virus genotypes: comparison of its performance vs a commercial primer. Anal Biochem 315:281–284PubMedCrossRef

13.

Guichon A, Chiparelli H, Martinez A, Rodriguez C, Trento A, Russi JC, Carballal G (2004) Evaluation of a new NASBA assay for the qualitative detection of hepatitis C virus based on the NucliSens Basic Kit reagents. J Clin Virol 29:84–91PubMedCrossRef

14.

Kleter GE, van Doorn LJ, Brouwer JT, Schalm SW, Heijtink RA, Quint WG (1994) Sequence analysis of the 5’ untranslated region in isolates of at least four genotypes of hepatitis C virus in The Netherlands. J Clin Microbiol 32:306–310PubMed

15.

Lan X, Yang B, Li BY, Yin XP, Li XR, Liu JX (2009) Reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis E virus. J Clin Microbiol 47:2304–2306PubMedCrossRef

16.

Le Guillou-Guillemette H, Lunel-Fabiani F (2009) Detection and quantification of serum or plasma HCV RNA: mini review of commercially available assays. Methods Mol Biol 510:3–14PubMedCrossRef

17.

Lu L, Nakano T, He Y, Fu Y, Hagedorn CH, Robertson BH (2005) Hepatitis C virus genotype distribution in China: predominance of closely related subtype 1b isolates and existence of new genotype 6 variants. J Med Virol 75:538–549PubMedCrossRef

18.

Meng S, Li J (2010) A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control. Virol J 7:117PubMedCrossRef

19.

Mori Y, Notomi T (2009) Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother 15:62–69PubMedCrossRef

20.

Murphy DG, Willems B, Deschenes M, Hilzenrat N, Mousseau R, Sabbah S (2007) Use of sequence analysis of the NS5B region for routine genotyping of hepatitis C virus with reference to C/E1 and 5’ untranslated region sequences. J Clin Microbiol 45:1102–1112PubMedCrossRef

21.

Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16:223–229PubMedCrossRef

22.

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63PubMedCrossRef

23.

Ozaras R, Tahan V (2009) Acute hepatitis C: prevention and treatment. Expert Rev Anti Infect Ther 7:351–361PubMedCrossRef

24.

Parida M, Posadas G, Inoue S, Hasebe F, Morita K (2004) Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42:257–263PubMedCrossRef

25.

Parida M, Sannarangaiah S, Dash PK, Rao PV, Morita K (2008) Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol 18:407–421PubMedCrossRef

26.

Sarrazin C, Gartner BC, Sizmann D, Babiel R, Mihm U, Hofmann WP, von Wagner M, Zeuzem S (2006) Comparison of conventional PCR with real-time PCR and branched DNA-based assays for hepatitis C virus RNA quantification and clinical significance for genotypes 1 to 5. J Clin Microbiol 44:729–737PubMedCrossRef

27.

Tomita N, Mori Y, Kanda H, Notomi T (2008) Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3:877–882PubMedCrossRef

28.

Tramarin A, Gennaro N, Compostella FA, Gallo C, Wendelaar Bonga LJ, Postma MJ (2008) HCV screening to enable early treatment of hepatitis C: a mathematical model to analyse costs and outcomes in two populations. Curr Pharm Des 14:1655–1660PubMedCrossRef

29.

Wei Y, Yang C, Wei B, Huang J, Wang L, Meng S, Zhang R, Li J (2008) RNase-resistant virus-like particles containing long chimeric RNA sequences produced by two-plasmid coexpression system. J Clin Microbiol 46:1734–1740PubMedCrossRef

30.

Xu SX, Lan K, Shan YL, Wang H, Luo JY, Cui F, Huang YS, Meng JP, Zhang XM, Yin YB (2007) Real-time quantitative assay of HCV RNA using the duplex scorpion primer. Arch Virol 152:431–440PubMedCrossRef

31.

Yoshida N, Fujino M, Miyata A, Nagai T, Kamada M, Sakiyama H, Ihara T, Kumagai T, Okafuji T, Nakayama T (2008) Mumps virus reinfection is not a rare event confirmed by reverse transcription loop-mediated isothermal amplification. J Med Virol 80:517–523PubMedCrossRef

Titel: Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay
verfasst von: Jin Yang
Mei-xin Fang
Jie Li
Guo-qiang Lou
Hang-jun Lu
Nan-ping Wu
Publikationsdatum: 01.08.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 8/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1001-4

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