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Erschienen in: Comparative Clinical Pathology 6/2015

01.11.2015 | Original Article

Detection of paratuberculosis using histopathology, immunohistochemistry, and ELISA in West Algeria

verfasst von: H. Hemida, M. Kihal

Erschienen in: Comparative Clinical Pathology | Ausgabe 6/2015

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Abstract

Paratuberculosis (Ptb), caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic enteritis that affects many ruminants and other wild animals worldwide. Although the prevalence of infection is unknown in nearly all countries, many studies report that paratuberculosis is the greatest economic problem in animal health. Here, we report the occurrence of paratuberculosis in herds of cattle in the Tiaret region of Algeria, using different diagnostic procedures. Tiaret and Sougueur abattoirs were being selected representing the main parts of the province. Four hundred intestines were examined from apparently healthy cattle that were slaughtered at Tiaret city (300) and Sougueur (100) slaughterhouses. Occurrence of subclinical paratuberculosis in cattle was 54, 51, 8, and 7.5 % using histopathology, IHC, ELISA, and ZN techniques, respectively. Histopathology is reported to be a better diagnostic tool mainly when complemented with immunohistochemistry.
Literatur
Zurück zum Zitat Al Hajri S, Alluwaimi A (2007) ELISA and PCR for evaluation of subclinical paratuberculosis in the Saudi dairy herds. Vet Microbiol 121:384–385CrossRefPubMed Al Hajri S, Alluwaimi A (2007) ELISA and PCR for evaluation of subclinical paratuberculosis in the Saudi dairy herds. Vet Microbiol 121:384–385CrossRefPubMed
Zurück zum Zitat Beard P, Rhind S, Buxton D, Daniels M, Henderson D, Pirie A, Rudge K, Greig A, Hutchings M, Stevenson K (2001) Natural paratuberculosis infection in rabbits in Scotland. J Comp Pathol 124:290–299CrossRefPubMed Beard P, Rhind S, Buxton D, Daniels M, Henderson D, Pirie A, Rudge K, Greig A, Hutchings M, Stevenson K (2001) Natural paratuberculosis infection in rabbits in Scotland. J Comp Pathol 124:290–299CrossRefPubMed
Zurück zum Zitat Bech-Nielsen S, Jorgensen JB, Ahrens P, Feld N (1992) Diagnostic accuracy of a Mycobacterium phlei-absorbed serum enzyme-linked immunosorbent assay for diagnosis of bovine paratuberculosis in dairy cows. J Clin Microbiol 30:613–618PubMedCentralPubMed Bech-Nielsen S, Jorgensen JB, Ahrens P, Feld N (1992) Diagnostic accuracy of a Mycobacterium phlei-absorbed serum enzyme-linked immunosorbent assay for diagnosis of bovine paratuberculosis in dairy cows. J Clin Microbiol 30:613–618PubMedCentralPubMed
Zurück zum Zitat Benazzi S, Berrada J, Schliesser T (1995) First report of paratuberculosis (Johne’s disease) in sheep in Morocco. J Veterinary Med Ser B 42:339–344CrossRef Benazzi S, Berrada J, Schliesser T (1995) First report of paratuberculosis (Johne’s disease) in sheep in Morocco. J Veterinary Med Ser B 42:339–344CrossRef
Zurück zum Zitat Billman‐Jacobe H, Carrigan M, Cockram F, Corner L, Gill I, Hill J, Jessep T, Milner A, Wood P (1992) A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne's disease in cattle. Aust Vet J 69:25–28CrossRefPubMed Billman‐Jacobe H, Carrigan M, Cockram F, Corner L, Gill I, Hill J, Jessep T, Milner A, Wood P (1992) A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne's disease in cattle. Aust Vet J 69:25–28CrossRefPubMed
Zurück zum Zitat Buergelt CD, Ginn PE (2000) The histopathologic diagnosis of subclinical Johne’s disease in North American Bison (Bison bison). Vet Microbiol 77:325–331CrossRefPubMed Buergelt CD, Ginn PE (2000) The histopathologic diagnosis of subclinical Johne’s disease in North American Bison (Bison bison). Vet Microbiol 77:325–331CrossRefPubMed
Zurück zum Zitat Buergelt C, Layton A, Ginn P, Taylor M, King J, Habecker P, Mauldin E, Whitlock R, Rossiter C, Collins M (2000) The pathology of spontaneous paratuberculosis in the North American bison (Bison bison). Vet Pathol 37:428–438CrossRefPubMed Buergelt C, Layton A, Ginn P, Taylor M, King J, Habecker P, Mauldin E, Whitlock R, Rossiter C, Collins M (2000) The pathology of spontaneous paratuberculosis in the North American bison (Bison bison). Vet Pathol 37:428–438CrossRefPubMed
Zurück zum Zitat Chiodini RJ, Van Kruiningen HJ, Merkal RS (1984) Ruminant paratuberculosis (Johne’s disease): the current status and future prospects. Cornell Vet 74:218–262 Chiodini RJ, Van Kruiningen HJ, Merkal RS (1984) Ruminant paratuberculosis (Johne’s disease): the current status and future prospects. Cornell Vet 74:218–262
Zurück zum Zitat Coetsier C, Havaux X, Mattelard F, Sadatte S, Cormont F, Buergelt K, Limbourg B, Latinne D, Bazin H, Denef J-F (1998) Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures. Clin Diagn Lab Immunol 5:446–451PubMedCentralPubMed Coetsier C, Havaux X, Mattelard F, Sadatte S, Cormont F, Buergelt K, Limbourg B, Latinne D, Bazin H, Denef J-F (1998) Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures. Clin Diagn Lab Immunol 5:446–451PubMedCentralPubMed
Zurück zum Zitat Colgrove G, Thoen C, Blackburn B, Murphy C (1989) Paratuberculosis in cattle: a comparison of three serologic tests with results of fecal culture. Vet Microbiol 19:183–187CrossRefPubMed Colgrove G, Thoen C, Blackburn B, Murphy C (1989) Paratuberculosis in cattle: a comparison of three serologic tests with results of fecal culture. Vet Microbiol 19:183–187CrossRefPubMed
Zurück zum Zitat Collins MT (1994) Clinical approach to control of bovine paratuberculosis. J-Am Vet Med Assoc 204:208PubMed Collins MT (1994) Clinical approach to control of bovine paratuberculosis. J-Am Vet Med Assoc 204:208PubMed
Zurück zum Zitat Collins MT, Kenefick KB, Sockett DC, Lambrecht RS, McDonald J, Jorgensen JB (1990) Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens. J Clin Microbiol 28:2514–2519PubMedCentralPubMed Collins MT, Kenefick KB, Sockett DC, Lambrecht RS, McDonald J, Jorgensen JB (1990) Enhanced radiometric detection of Mycobacterium paratuberculosis by using filter-concentrated bovine fecal specimens. J Clin Microbiol 28:2514–2519PubMedCentralPubMed
Zurück zum Zitat Collins M, Sockett D, Ridge S, Cox J (1991) Evaluation of a commercial enzyme-linked immunosorbent assay for Johne's disease. J Clin Microbiol 29:272–276PubMedCentralPubMed Collins M, Sockett D, Ridge S, Cox J (1991) Evaluation of a commercial enzyme-linked immunosorbent assay for Johne's disease. J Clin Microbiol 29:272–276PubMedCentralPubMed
Zurück zum Zitat Corpa J, Garrido J, Garcıa Marın J, Perez V (2000) Classification of lesions observed in natural cases of paratuberculosis in goats. J Comp Pathol 122:255–265CrossRefPubMed Corpa J, Garrido J, Garcıa Marın J, Perez V (2000) Classification of lesions observed in natural cases of paratuberculosis in goats. J Comp Pathol 122:255–265CrossRefPubMed
Zurück zum Zitat Djønne B, Jensen M, Grant I, Holstad G (2003) Detection by immunomagnetic PCR of Mycobacterium avium subsp. paratuberculosis in milk from dairy goats in Norway. Vet Microbiol 92:135–143CrossRefPubMed Djønne B, Jensen M, Grant I, Holstad G (2003) Detection by immunomagnetic PCR of Mycobacterium avium subsp. paratuberculosis in milk from dairy goats in Norway. Vet Microbiol 92:135–143CrossRefPubMed
Zurück zum Zitat Green E, Tizard M, Moss M, Thompson J, Winterbourne D, McFadden J, Hermon-Taylor J (1989) Sequence and characteristics or IS900, an insertion element identified in a human Crohn's disease isolate or Mycobacterium paratuberculosis. Nucleic Acids Res 17:9063–9073PubMedCentralCrossRefPubMed Green E, Tizard M, Moss M, Thompson J, Winterbourne D, McFadden J, Hermon-Taylor J (1989) Sequence and characteristics or IS900, an insertion element identified in a human Crohn's disease isolate or Mycobacterium paratuberculosis. Nucleic Acids Res 17:9063–9073PubMedCentralCrossRefPubMed
Zurück zum Zitat Hailat N, Hananeh W, Metekia A, Stabel J, Al-Majali A, Lafi S (2010) Pathology of subclinical paratuberculosis (Johne’s disease) in Awassi sheep with reference to its occurrence in Jordan. Vet Med 55:590–602 Hailat N, Hananeh W, Metekia A, Stabel J, Al-Majali A, Lafi S (2010) Pathology of subclinical paratuberculosis (Johne’s disease) in Awassi sheep with reference to its occurrence in Jordan. Vet Med 55:590–602
Zurück zum Zitat Hailat N, Hemida H, Hananeh W, Stabel J, Rezig F, Jaradat S, Al-Saleh A-A (2012) Investigation on the occurrence and pathology of paratuberculosis (Johne’s disease) in apparently healthy cattle in Jordan. Comp Clin Pathol 21:879–888CrossRef Hailat N, Hemida H, Hananeh W, Stabel J, Rezig F, Jaradat S, Al-Saleh A-A (2012) Investigation on the occurrence and pathology of paratuberculosis (Johne’s disease) in apparently healthy cattle in Jordan. Comp Clin Pathol 21:879–888CrossRef
Zurück zum Zitat Huda A, Jensen H (2003) Comparison of histopathology, cultivation of tissues and rectal contents, and interferon-gamma and serum antibody responses for the diagnosis of bovine paratuberculosis. J Comp Pathol 129:259–267CrossRefPubMed Huda A, Jensen H (2003) Comparison of histopathology, cultivation of tissues and rectal contents, and interferon-gamma and serum antibody responses for the diagnosis of bovine paratuberculosis. J Comp Pathol 129:259–267CrossRefPubMed
Zurück zum Zitat Johnson-Ifearulundu Y, Kaneene J, Sprecher D, Gardiner J, Lloyd J (2000) The effect of subclinical Mycobacterium paratuberculosis infection on days open in Michigan, USA, dairy cows. Prev vet Med 46:171–181CrossRefPubMed Johnson-Ifearulundu Y, Kaneene J, Sprecher D, Gardiner J, Lloyd J (2000) The effect of subclinical Mycobacterium paratuberculosis infection on days open in Michigan, USA, dairy cows. Prev vet Med 46:171–181CrossRefPubMed
Zurück zum Zitat Kurade N, Tripathi B, Rajukumar K, Parihar N (2004) Sequential development of histologic lesions and their relationship with bacterial isolation, fecal shedding, and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. paratuberculosis. Vet Pathol 41:378–387CrossRefPubMed Kurade N, Tripathi B, Rajukumar K, Parihar N (2004) Sequential development of histologic lesions and their relationship with bacterial isolation, fecal shedding, and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. paratuberculosis. Vet Pathol 41:378–387CrossRefPubMed
Zurück zum Zitat Lambrecht RS, Carriere JF, Collins MT (1988) A model for analyzing growth kinetics of a slowly growing Mycobacterium sp. Appl Environ Microbiol 54:910–916PubMedCentralPubMed Lambrecht RS, Carriere JF, Collins MT (1988) A model for analyzing growth kinetics of a slowly growing Mycobacterium sp. Appl Environ Microbiol 54:910–916PubMedCentralPubMed
Zurück zum Zitat Lee H, Stabel J, Kehrli M Jr (2001) Cytokine gene expression in ileal tissues of cattle infected with Mycobacterium paratuberculosis. Vet Immunol Immunopathol 82:73–85CrossRefPubMed Lee H, Stabel J, Kehrli M Jr (2001) Cytokine gene expression in ileal tissues of cattle infected with Mycobacterium paratuberculosis. Vet Immunol Immunopathol 82:73–85CrossRefPubMed
Zurück zum Zitat Mahmoud O, Haroun E, Elfaki M, Abbas B (2002) Pigmented paratuberculosis granulomata in the liver of sheep. Small Rumin Res 43:211–217CrossRef Mahmoud O, Haroun E, Elfaki M, Abbas B (2002) Pigmented paratuberculosis granulomata in the liver of sheep. Small Rumin Res 43:211–217CrossRef
Zurück zum Zitat Manning EJ, Steinberg H, Krebs V, Collins MT (2003) Diagnostic testing patterns of natural Mycobacterium paratuberculosis infection in pygmy goats. Can J Vet Res 67:213PubMedCentralPubMed Manning EJ, Steinberg H, Krebs V, Collins MT (2003) Diagnostic testing patterns of natural Mycobacterium paratuberculosis infection in pygmy goats. Can J Vet Res 67:213PubMedCentralPubMed
Zurück zum Zitat Martinson SA, Hanna PE, Ikede BO, Lewis JP, Miller LM, Keefe GP, McKenna SL (2008) Comparison of bacterial culture, histopathology, and immunohistochemistry for the diagnosis of Johne’s disease in culled dairy cows. J Vet Diagn Investig 20:51–57CrossRef Martinson SA, Hanna PE, Ikede BO, Lewis JP, Miller LM, Keefe GP, McKenna SL (2008) Comparison of bacterial culture, histopathology, and immunohistochemistry for the diagnosis of Johne’s disease in culled dairy cows. J Vet Diagn Investig 20:51–57CrossRef
Zurück zum Zitat Merkal R, Curran B (1974) Growth and metabolic characteristics of Mycobacterium paratuberculosis. Appl Microbiol 28:276–279PubMedCentralPubMed Merkal R, Curran B (1974) Growth and metabolic characteristics of Mycobacterium paratuberculosis. Appl Microbiol 28:276–279PubMedCentralPubMed
Zurück zum Zitat Momotani E, Romona NM, Yoshihara K, Momotani Y, Hori M, Ozaki H, Eda S, Ikegami M (2012) Molecular pathogenesis of bovine paratuberculosis and human inflammatory bowel diseases. Vet Immunol Immunopathol 148:55–68CrossRefPubMed Momotani E, Romona NM, Yoshihara K, Momotani Y, Hori M, Ozaki H, Eda S, Ikegami M (2012) Molecular pathogenesis of bovine paratuberculosis and human inflammatory bowel diseases. Vet Immunol Immunopathol 148:55–68CrossRefPubMed
Zurück zum Zitat Nielsen SS, Enevoldsen C, Gröhn YT (2002) The Mycobacterium avium subsp. paratuberculosis ELISA response by parity and stage of lactation. Pre Vet Med 54:1–10CrossRef Nielsen SS, Enevoldsen C, Gröhn YT (2002) The Mycobacterium avium subsp. paratuberculosis ELISA response by parity and stage of lactation. Pre Vet Med 54:1–10CrossRef
Zurück zum Zitat Nielsen SS, Thamsborg SM, Houe H, Bitsch V (2000) Bulktank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. Prev Vet Med 44:1–7 Nielsen SS, Thamsborg SM, Houe H, Bitsch V (2000) Bulktank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. Prev Vet Med 44:1–7
Zurück zum Zitat Perez V, Marín J, Badiola J (1996) Description and classification of different types of lesion associated with natural paratuberculosis infection in sheep. J Comp Pathol 114:107–122CrossRefPubMed Perez V, Marín J, Badiola J (1996) Description and classification of different types of lesion associated with natural paratuberculosis infection in sheep. J Comp Pathol 114:107–122CrossRefPubMed
Zurück zum Zitat Perez V, Tellechea J, Corpa J, Gutierrez M, García MJ (1999) Relation between pathologic findings and cellular immune responses in sheep with naturally acquired paratuberculosis. Am J Vet Res 60:123–127PubMed Perez V, Tellechea J, Corpa J, Gutierrez M, García MJ (1999) Relation between pathologic findings and cellular immune responses in sheep with naturally acquired paratuberculosis. Am J Vet Res 60:123–127PubMed
Zurück zum Zitat Raizman E, Fetrow J, Wells S (2009) Loss of income from cows shedding Mycobacterium avium subspecies paratuberculosis prior to calving compared with cows not shedding the organism on two Minnesota dairy farms. J Dairy Sci 92:4929–4936CrossRefPubMed Raizman E, Fetrow J, Wells S (2009) Loss of income from cows shedding Mycobacterium avium subspecies paratuberculosis prior to calving compared with cows not shedding the organism on two Minnesota dairy farms. J Dairy Sci 92:4929–4936CrossRefPubMed
Zurück zum Zitat Ris D, Hamel K, Ayling J (1988) The detection of Mycobacterium paratuberculosis in bovine faeces by isolation and the comparison of isolation with the examination of stained smears by light microscopy. N Z Vet J 36:112–114CrossRefPubMed Ris D, Hamel K, Ayling J (1988) The detection of Mycobacterium paratuberculosis in bovine faeces by isolation and the comparison of isolation with the examination of stained smears by light microscopy. N Z Vet J 36:112–114CrossRefPubMed
Zurück zum Zitat Salem M, Zeid A, Hassan D, El‐Sayed A, Zschoeck M (2005) Studies on Johne’s disease in Egyptian cattle. J Veterinary Med Ser B 52:134–137CrossRef Salem M, Zeid A, Hassan D, El‐Sayed A, Zschoeck M (2005) Studies on Johne’s disease in Egyptian cattle. J Veterinary Med Ser B 52:134–137CrossRef
Zurück zum Zitat Sigurdardottir O, Press CM, Saxegaard F, Evensen Ø (1999) Bacterial isolation, immunological response, and histopathological lesions during the early subclinical phase of experimental infection of goat kids with Mycobacterium avium subsp. paratuberculosis. Vet Pathol 36:542–550CrossRefPubMed Sigurdardottir O, Press CM, Saxegaard F, Evensen Ø (1999) Bacterial isolation, immunological response, and histopathological lesions during the early subclinical phase of experimental infection of goat kids with Mycobacterium avium subsp. paratuberculosis. Vet Pathol 36:542–550CrossRefPubMed
Zurück zum Zitat Stabel JR (1997) An improved method for cultivation of Mycobacterium paratuberculosis from bovine fecal samples and comparison to three other methods. J Vet Diagn Investig 9:375–380CrossRef Stabel JR (1997) An improved method for cultivation of Mycobacterium paratuberculosis from bovine fecal samples and comparison to three other methods. J Vet Diagn Investig 9:375–380CrossRef
Zurück zum Zitat Stabel J, Wells S, Wagner B (2002) Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne’s disease in US dairy herds. J Dairy Sci 85:525–531CrossRefPubMed Stabel J, Wells S, Wagner B (2002) Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne’s disease in US dairy herds. J Dairy Sci 85:525–531CrossRefPubMed
Zurück zum Zitat Sternberg S, Viske D (2002) Control strategies for paratuberculosis in Sweden. Acta Vet Scand 44:247–249 Sternberg S, Viske D (2002) Control strategies for paratuberculosis in Sweden. Acta Vet Scand 44:247–249
Zurück zum Zitat Thoresen OF, Falk K, Evensen Ø (1994) Comparison of immunohistochemistry, acid-fast staining, and cultivation for detection of Mycobacterium paratuberculosis in goats. J Vet Diagn Investig 6:195–199CrossRef Thoresen OF, Falk K, Evensen Ø (1994) Comparison of immunohistochemistry, acid-fast staining, and cultivation for detection of Mycobacterium paratuberculosis in goats. J Vet Diagn Investig 6:195–199CrossRef
Zurück zum Zitat Van der Giessen J, Haring R, Vauclare E, Eger A, Haagsma J, Van der Zeijst B (1992) Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle: application in a control program. J Clin Microbiol 30:1216–1219PubMedCentralPubMed Van der Giessen J, Haring R, Vauclare E, Eger A, Haagsma J, Van der Zeijst B (1992) Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle: application in a control program. J Clin Microbiol 30:1216–1219PubMedCentralPubMed
Metadaten
Titel
Detection of paratuberculosis using histopathology, immunohistochemistry, and ELISA in West Algeria
verfasst von
H. Hemida
M. Kihal
Publikationsdatum
01.11.2015
Verlag
Springer London
Erschienen in
Comparative Clinical Pathology / Ausgabe 6/2015
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-015-2126-8

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