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22.04.2020 | Original Article

Detection of trifluridine in tumors of patients with metastatic colorectal cancer treated with trifluridine/tipiracil

verfasst von: Yoshiaki Fujimoto, Ryota Nakanishi, Mamoru Nukatsuka, Kazuaki Matsuoka, Koji Ando, Takeshi Wakasa, Hiroyuki Kitao, Eiji Oki, Yoshihiko Maehara, Masaki Mori

Erschienen in: Cancer Chemotherapy and Pharmacology | Ausgabe 6/2020

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Abstract

Purpose

Trifluridine (FTD) is the active component of the nucleoside chemotherapeutic drug trifluridine/tipiracil (FTD/TPI), which is approved worldwide for the treatment of patients with metastatic gastrointestinal cancer. FTD exerts cytotoxic effects via its incorporation into DNA, but FTD has not been detected in the tumor specimens of patients. The purpose of this study was to detect FTD in tumors resected from metastatic colorectal cancer (mCRC) patients who were administered FTD/TPI. Another purpose was to investigate the turnover rate of FTD in tumors and bone marrow in a mouse model.

Methods

Tumors and normal tissue specimens were obtained from mCRC patients who were administered FTD/TPI or placebo at Kyushu University Hospital. Tumors and bone marrow were resected from mice with peritoneal dissemination treated with FTD/TPI. To detect and quantitate FTD incorporated into DNA, immunohistochemical staining of paraffin-embedded specimens (IHC-p staining) and slot-blot analysis of DNA purified from these tissues were performed using an anti-BrdU antibody. IHC-p staining of proliferation and apoptosis markers was also performed.

Results

FTD was detected in metastatic tumors obtained from mCRC patients who were administered FTD/TPI, but who had discontinued the treatment several weeks before surgery. In a peritoneal dissemination mouse model, FTD was still detected in tumors 13 days after the cessation of FTD/TPI treatment, but had disappeared from bone marrow within 6 days.

Conclusion

These results indicate that FTD persists longer in tumors than in bone marrow, which may cause a sustained antitumor effect with tolerable hematotoxicity.

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Titel: Detection of trifluridine in tumors of patients with metastatic colorectal cancer treated with trifluridine/tipiracil
verfasst von: Yoshiaki Fujimoto
Ryota Nakanishi
Mamoru Nukatsuka
Kazuaki Matsuoka
Koji Ando
Takeshi Wakasa
Hiroyuki Kitao
Eiji Oki
Yoshihiko Maehara
Masaki Mori
Publikationsdatum: 22.04.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: Cancer Chemotherapy and Pharmacology / Ausgabe 6/2020
Print ISSN: 0344-5704
Elektronische ISSN: 1432-0843
DOI: https://doi.org/10.1007/s00280-020-04072-6

Springer Medizin

Detection of trifluridine in tumors of patients with metastatic colorectal cancer treated with trifluridine/tipiracil