Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 8/2009

01.08.2009 | Animal Experimentation in Assisted Reproduction

Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium

verfasst von: Uttam Datta, M. Chandra Sekar, Manik Lal Hembram, Raju Dasgupta

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2009

Einloggen, um Zugang zu erhalten

Abstract

Purpose

In-situ preservation of cauda epididymal spermatozoa at -10°C with electrolyte free media for obtaining maximum functional gametes than preservation at 5°C.

Methods

Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 — glycerol and soybean lecithin — Coenzyme Q10— glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5°C, wrapped with aluminium foil and freezed at — 10°C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5°C in a water bath for 5 min.

Results

Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 ± 0.62; 45.2 ± 0.82 and 41.6 ± 0.79 percent respectively on day 21.

Conclusion

This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter.
Literatur
1.
2.
Anel L, Guerra C, Alvarez M, Kaabi M, Anel E, Boixo JC, et al. Basic parameters in spermatozoa recovered post-mortem from the Spanish Cantabrian Chamois (Rupicapra pyrenaica parva). Theriogenology. 2000;53(1):323. abstract.
3.
Blash S, Melican D, Gavin W. Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology. 2000;54(6):899–905.CrossRefPubMed
4.
Kilian I, Lubbe K, Bartels P, Friedmann Y, Denniston RS. Evaluating epididymal sperm of African wild ruminants: Longevity when stored at 4°C and viability following cryopreservation. Theriogenology. 2000;53(1):330. abstract.
5.
Lubbe K, Bartels P, Kilian I, Friedmann Y, Godke RA. Comparing motility and morphology of horse, zebra and rhinoceros epididymal spermatozoa when cryopreserved with two different cryodiluents or stored at 4°C. Theriogenology. 2000;53(1):338. abstract.
6.
Soler AJ, Perez-guzman MD, Grade J. Storage of red deer epididymides for four days at 5°C: effects on motility, viability and morphological integrity. J. Exp. Zool. Part A: Comparative Exp. Biol. 2003;295A:188–99.CrossRef
7.
Martinez-Pastor F, Guerra C, Kabbi M, Diaz AR, Anel E, Herraez P, et al. Decay of sperm obtained from epididymis of wild ruminants depending on postmortem time. Theriogenology. 2005;63(1):24–40.CrossRefPubMed
8.
Dong Q, Rodenberg SE, Huang C, Vandevoort CA. Cryopreservation of Rhesus monkey (Macaca muitatta) epididymal spermatozoa before and after refrigerated storage. J. Androl. 2008;29(3):283–92.CrossRefPubMed
9.
James AN, Green H, Hoffman S, Landry AM, Paccamonti D, Godke RA. Preservation of equine sperm stored in the epididymides at 4°C for 24, 48, 72 and 96 Hours. Theriogenology. 2002;58:401–4.CrossRef
10.
Yu I, Leibo SP. Recovery of motile, membrane-intact spermatozoa from canine epididymides stored for 8 days at 4°C. Theriogenology. 2002;57:1179–90.CrossRefPubMed
11.
Fournier-Delpech S, Colas G, Courot M, Ortavant R, Brice G. Epididymal sperm maturation in the ram: motility, fertilizing ability and embryonic survival after uterine artificial insemination. Ann. Biol. Anim. Bioch. Biophys. 1979;19(3A):597–605.CrossRef
12.
Niwa K, Ohara K, Hosoi Y, Iritani A. Early events of in-vitro fertilization of cat eggs by epididymal spermatozoa. J. Reprod. Fertil. 1985;74(2):657–60.PubMed
13.
Temple-Smith PD, Southwick GJ, Yates CA, Trounson AO, de Kretser DM. Human pregnancy by in vitro fertilization (IVF) using sperm aspirated from the epididymis. J. In Vitro Fertil. Embryo Transfer. 1985;2:119–222.CrossRef
14.
Marks SL, Dupuis J, Mickelsen WD, Memon MA, Platz CC. Conception by use of postmortem epididymal semen extraction in a dog. J. Anim. Vet. Med. Assoc. 1994;204(10):1639–40.
15.
Graff KJ, Chandler JE, Reggio BC, Lim JM, Canal A, Carter JA, Meintjes M, Godke RA: Pregnancies obtained from IVF with noncapacitated epididymal bovine spermatozoa. Proc. 3rd Intern’l. Mtg. Biotech. Anim. Reprod. Cairo, Egypt (Nov. 2–6). 1996: pp. 19–21.
16.
Bonduel M, Wilikens A, Buysse A, Van Assche E, Devroey P, Van Steirteghem AC, et al. A follow-up study of children born after intracytoplasmic sperm injection (ICSI) with epididymal and testicular spermatozoa and after replacement of cryopreserved embryos obtained after ICSI. Hum. Reprod. 1998;13(Suppl 1):196–207.
17.
Morrell JM, Nubbemeyer R, Heistermann M, Rosenbusch J, Kuderling I, Holt W, et al. Artificial insemination in Callithrix jacchus using fresh or cryopreserved sperm. Anim. Reprod. Sci. 1998;52(2):165–74.CrossRefPubMed
18.
Bartels P, Lubbe K, Kilian I, Friedmann Y, van Dyk G, Mortimer D. In vitro maturation and fertilization of lion (Panthera leo) oocytes using frozen-thawed epididymal spermatozoa recovered by cauda epididymectomy of an immobilized lion. Theriogenology. 2000;53(1):325. abstract.
19.
Kusunoki H, Daimaru H, Miami S, Nishimoto S, Yamane K-I, Fukumoto Y. Birth of a chimpanzee (Pan troglodytes) after artificial insemination with cryopreserved epididymal spermatozoa collected postmortem. Zoo. Biol. 2001;20:135–43.CrossRef
20.
Ikeda H, Kikuchi K, Noguchi J, Takeda H, Shimada A, Mizokami T, et al. Effect of preincubation of cryopreserved porcine epididymal sperm. Theriogenology. 2002;57:1309–18.CrossRefPubMed
21.
Martins CF, Rumpf R, Pereira DC, Dode MN. Cryopreservation of epididymal bovine spermatozoa from dead animals and its use in in-vitro embryo production. Anim. Reprod. Sci. 2007;101:326–31.CrossRefPubMed
22.
Said S, dan Saili T. Rescuing genetic material of unexpectedly die animal. Jurnal Ilmu Ternak dan Veteriner. 2007;12(2):147–52.
23.
World health Organization: WHO Laboratory manual for the examinations of human semen and sperm-cervical mucus interaction (3e). Cambridge; The press syndicated of the University of Cambridge. 1997.
24.
Sidhu KS, Guraya SS: In: Buffalo bull semen morphology, biochemistry, physiology and methodology. USA publishers and distributors, Ludhiana, India. 1985a; 152–154.
25.
Jeyendran RS, Vandervent HH, Perez-Paleac ZM, Crabo BG, Zaneveld LJD. Development of an assay to assess the functional integrity of human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 1984;70:219–25.PubMedCrossRef
26.
Duncan DB. Multiple range and multiple F tests. Biometrics. 1955;11:1–42.CrossRef
27.
Scott TW, Voglmayer JK, Setchell BP. Lipid composition and metabolism in testicular and ejaculated spermatozoa. The Biochemical Journal. 1967;102:456–60.PubMed
28.
Arora R, Dinakar N, Prasad MRN. Biochemical changes in the spermatozoa and luminal contents of different regions of the epididymis of the rhesus monkey, Macaca mulalta. Contraception. 1975;11:689–700.CrossRefPubMed
29.
Hammerstedt RH, Keith AD, Hay S, Deluca N, Amann RP. Changes in ram sperm membrane during epididymis transit. Arch. Biochem. Biophys. 1979;196:7–12.CrossRefPubMed
30.
Thun R, Hurtado M, Janett F. Comparison of Biociphos-plus and TRIS-egg yolk extender for cryopreservation of bull semen. Theriogenology. 2002;57:1087–94.CrossRefPubMed
31.
Mancini A, Marinis LD, Oradei A, Hallgass ME, Conte G, Pozza D, et al. Coenzyme Q10 concentration in normal and pathological Human seminal fluid’s. J. Androl. 1994;15(6):591–4.PubMed
32.
Jones R, Mann T. Damage to ram spermatozoa by peroxidation of endogenous phospholipids. J.Reprod. Fertil. 1977;50:261–8.PubMed
33.
Ernster L, Forsneark-Andree P. Ubiquinol: an endogenous antioxidant in aerobic organisms. Clin. Invest. 1993;71:S60–65.CrossRef
34.
Ernster L, Dallner G. Biochemical, physiological and medical aspects of ubiquinone function. Biochem. Biophys. Acta. 1995;1271:195–204.PubMed
35.
Lewin A, Lavon H. The effect of Coenzyme Q10 on sperm motility and function. Molecular Aspects of Medicine. 1997;18(Suppl):S 213–9.
36.
Thomas SR, Neuzil J, Stocker R. Co supplementation with coenzyme Q prevents the prooxidant effects of alphatocopherol and increase the resistance of LDL to transition metal-dependent oxidation initiation. Arterioscler. Thromb. Vasc. Biol. 1996;16:687–96.PubMed
37.
Thomas SR, Neuzil J, Stocker R. Inhibition of LDL oxidation by ubiquinone-10. A protective mechanism for coenzyme Q in atherogenesis? Mol.Aspects. Med. 1997;18:S85–103.CrossRefPubMed
38.
Booth NH, McDonald LE. Veterinary pharmacology and Therapeutics. 6th ed. New Delhi: Kalyani publisher; 1982.
39.
Mazzilli F, Cerasaro M, Bisanti A, Rossi T, Dondero F: Seminal parameters and the swelling test in patients with sperm before and after treatment with ubiquinone (CoQ10). 2nd international symposium on reproductive medicine. Acta Medica, Edizioni e Congresi, Rome, Italy: Fiuggi. 1988; 71.
40.
Ball BA, Vo AT, Baumber J. Reactive oxygen species generation by equine spermatozoa. Am. J. Vet. Res. 2001;62:5508–15.CrossRef
41.
Hammerstedt RH, Graham JK, Nolan JP. Cryopreservation of mammalian sperm: What we ask them to survive. J. Androl. 1990;11:73–88.PubMed
42.
Riddle VM, Lorenz N. Nonenzymic formulation of toxic levels of methylglyoxal from glycerol and dihydroxyacetone in Ringers phosphate suspensions of avian spermatozoa. Biochem, Biophy. Res. Commun. 1973;50:27–34.CrossRef
43.
Fahy GM, Lilley TH, Linsdell H, Douglas MS, Meryman HT. Cryoprotectant toxicity and cryoprotectant toxicity reduction: In search of molecular mechanisms. Cryobiol. 1990;27:247–68.CrossRef
44.
Watson PF. The effects of cold shock on sperm cell membranes. In: Morris GJ, Clark A, editors. Effects of Low temperatures on Biological membranes. London: Academic; 1981. p. 189–218.
45.
Rodojcic L, Vukotic-Maletic V, Balint B. Current Knowledge on cryopreservation of spermattzoa, ovum cells and zygotes. Medicinski pregled. 1998;51(1–2):29–36.
46.
Curry MR, Watson PF. Osmotic effects on ram and human sperm membranes in relation to thawing injury. Cryobiology. 1994;31(1):39–46.CrossRefPubMed
47.
Watson PF. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertility. Dev. 1995;7:747–869.CrossRef
48.
Harrison RAP, White IG. Glycolytic enzymes in the spermatozoa and cytoplasmic droplets of bull, boar and ram and their leakage after shock. J Reprod Fertil. 1972;30:105–15.PubMedCrossRef
49.
Simpson AM, White IG. Effect of cold shock and cooling rate on calcium uptake of ram spermatozoa. Anim. Reprod. Sci. 1986;12:131–43.CrossRef
50.
Robertson L, Bailey JL, Buhr MM. Effects of cold shock and phospholipase A2 on intact boar spermatozoa and sperm head plasma membranes. Mol. Reprod Dev. 1990;26:143–9.CrossRefPubMed
51.
Cotran RS, Kumar V, Robbins SL. Robbin’s Pathologic basis of disease. 4th ed. Philadelphia: WB Saunders Co; 1989. p. 9–16.
52.
Bilodeau JF, Chatterjee S, Sired MA, Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol. Reprod. Dev. 2000;55:282–8.CrossRefPubMed
53.
Quinn PJ. Principles of membrane stability and phase behavior under extreme conditions. J Bioenerg Biomembr. 1989;21:3–19.CrossRefPubMed
54.
Holt WV, North RD. The role of membrane —active lipids in the protection of ram spermatozoa during cooling and storage. Gamete Res. 1988;19:77–89.CrossRefPubMed
55.
De Leeuw FE, Chen HC, Colenbrander B, Verkleji AJ. Cold-induced ultra structural changes in bull and boar sperm plasma membranes. Cryobiology. 1990;27:171–83.CrossRefPubMed
56.
Buhr MM, Curtis EF, Kakuda NS. Composition and behavior of head membrane lipids of fresh and cryopreserved boar sperm. Cryobiology. 1994;31:224–38.CrossRefPubMed
57.
White IG. Lipids and calcium uptake of sperm in relation to cold shock and preservation: A review. Reproduction. Fertility. Dev. 1993;5(6):639–58.CrossRef
58.
Aitken RJ. Free radicals, lipid peroxidation and sperm function. Reprod. Fertil. Dev. 1995;7:659–68.CrossRefPubMed
59.
Drobinis EZ, Crowe LM, Berger T, Anchordoguy TJ, Overstreet JW, Crowe JH. Cold shock damage is due to lipid phase transitions in cell-membranes-a demonstration using sperm as a model. J. Exp. Zool. 1993;265:432–7.CrossRef
60.
Rao B, Soufir JC, Martin M. Lipid peroxidation in human spermatozoa as related to mid piece abnormalities and motility. Gamete Res. 1989;24:127–34.CrossRefPubMed
61.
Aitken RJ, West K, Buckingham DW. Leukocytic infiltration into the human ejaculate and its association with semen quality, oxidative stress, and sperm function. J. Androl. 1994;15:343–52.PubMed
62.
Baumber J, Ball BA, Gravance CG, Medina V, Davies-Morel MC. The effect of reactive species on equine sperm motility, viability, acrosomal integrity, mitochondria membrane potential, and membrane lipid peroxidation. J.Androl. 2001;21:895–902.
Metadaten
Titel
Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium
verfasst von
Uttam Datta
M. Chandra Sekar
Manik Lal Hembram
Raju Dasgupta
Publikationsdatum
01.08.2009
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 8/2009
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-009-9344-4

Weitere Artikel der Ausgabe 8/2009

Journal of Assisted Reproduction and Genetics 8/2009 Zur Ausgabe

Assisted Reproduction

The influence of early cleavage on embryo developmental potential and IVF/ICSI outcome

SHORT COMMUNICATION

Is gestation in Prader-Willi syndrome affected by the genetic subtype?

Assisted Reproduction

Top quality embryos at day 2: a prerequisite for single blastocyst transfer? An observational cohort study in women under 36

ORIGINAL ARTICLE

Blastocyst transfer and gender: IVF versus ICSI

GENETICS

Prenatal diagnosis of skeletal dysplasia due to FGFR3 gene mutations: a 9-year experience

Minireview

Antioxidants and sperm DNA damage: a clinical perspective

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hirsutismus bei PCOS: Laser- und Lichttherapien helfen

26.04.2024 Hirsutismus Nachrichten

Laser- und Lichtbehandlungen können bei Frauen mit polyzystischem Ovarialsyndrom (PCOS) den übermäßigen Haarwuchs verringern und das Wohlbefinden verbessern – bei alleiniger Anwendung oder in Kombination mit Medikamenten.

ICI-Therapie in der Schwangerschaft wird gut toleriert

23.04.2024 Medikamente in Schwangerschaft und Stillzeit Nachrichten

Müssen sich Schwangere einer Krebstherapie unterziehen, rufen Immuncheckpointinhibitoren offenbar nicht mehr unerwünschte Wirkungen hervor als andere Mittel gegen Krebs.

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

23.04.2024 Depression antepartal oder postpartal Nachrichten

Bislang gibt es kein Medikament zur Prävention von Wochenbettdepressionen. Das Injektionsanästhetikum Esketamin könnte womöglich diese Lücke füllen.

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

22.04.2024 DGIM 2024 Kongressbericht

Durch die Häufung nach der COVID-19-Pandemie sind Infektionen mit dem Respiratorischen Synzytial-Virus (RSV) in den Fokus gerückt. Fachgesellschaften empfehlen eine Impfung inzwischen nicht nur für Säuglinge und Kleinkinder.

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen
Bildnachweise