01.12.2017 | Research article | Ausgabe 1/2017 Open Access

Dickkopf-4 is frequently overexpressed in epithelial ovarian carcinoma and promotes tumor invasion
- Zeitschrift:
- BMC Cancer > Ausgabe 1/2017
Background
Methods
Clinical samples
Quantitative real-time RT-PCR
Western blot
Immunohistochemistry analysis
siRNA transfection for DKK4 silence in vitro
Transwell invasion assay
Drugs and reagents
Filamentous actin staining
Statistical analysis
Results
DKK4 mRNA and protein were over-expressed in ovarian cancer
The prognostic significance of DKK4 protein in epithelial ovarian patients
No.
|
DKK-4 expression
|
||||
---|---|---|---|---|---|
+/−
|
++
|
value
|
P
|
||
Age
|
|||||
≤ 45
|
102
|
41
|
61
|
0.339
|
0.592
|
> 45
|
137
|
50
|
87
|
||
FIGO stage
|
|||||
I ~ II
|
105
|
51
|
54
|
8.750
|
0.005
|
III ~ IV
|
134
|
40
|
94
|
||
Differentiation
|
|||||
G1 ~ G2
|
111
|
46
|
65
|
0.996
|
0.351
|
G3
|
128
|
45
|
83
|
||
Lymph metastasis
|
|||||
no
|
168
|
61
|
107
|
0.748
|
0.387
|
yes
|
71
|
30
|
41
|