Background
Methods
Tissue samples and RNA isolation
Affymetrix GeneChip Array
Semiquantitative RT-PCR
Rapid amplification of cDNA ends (RACE) and sequencing
PCR and sequencing primers | ||
---|---|---|
Name | Sequence 5'->3' | |
RT-PCR | DLG2_spec1-F | GATGACCCTGGCATATTTATTACGA |
DLG2_spec1-R | ACGATAGACCCTGCTTCCTTCA | |
DLG2_spec2-F | TGGGGAACCAACACATTCCT | |
DLG2_spec2-R | TTGCTACTGCCTCTTCGTGTG | |
Intergene_spec-F | GGCCAAAATGCTAAATGGTCCT | |
Intergene_spec-R | CCTCACTTACCTCCCTTCCACA | |
Affy_DLG2-F | GCTCTATCCCATTGCCATCTT | |
Affy_DLG2-R | CGTTGTCAGAGGCGCAGTAT | |
Affy_FLJ37266-F | TGCCAGATCGTGTAACTGGTG | |
Affy_FLJ37266-R | TAGCCAAGAAATCCCGCAAA | |
RT-PCR controls | GAPDH-F | GGAAGGTGAAGGTCGGAGTCAAC |
GAPDH-R | CCACTTGATTTTGGAGGGATCTCG | |
β-actin-F | ATGGATGATGATATCGCCGCGC | |
β-actin-R | TGATGGTGGGCATGGGTCAGAA | |
Sequencing | Amp_F | CGTCTCTAACAGAGGAACAAGCCAAG |
Amp_R | GGAGTGTAAGCTGTGCAGTGTCTC | |
DLG2_3'-F_2_ IRD800 | GAAGCCATGAAGGAAACAGAGG | |
DLG2_3'-F_IRD700 | TTGATTGCCTATTTTGCCATTT | |
DLG2_5'-F_ IRD700 | ACCAGCTTTCGCTATGCAAC | |
FLJ37266-F_IRD800 | TGTTGATGTCTCTATGGCTCCA | |
FLJ37266-R_IRD700 | TGGAGCCATAGAGACATCAACA | |
LD-PCR | FLJ37266_universal-R | CAATTCTGTGGGAGGAGAGCAA |
DLG_exon22-F | GGTGACGACGGTTATGGAACAA | |
DLG_exon21-F | ACAAGGAGCAGAGTGAGCAGGA | |
DLG_exon20-F | GTGCCCGATTGAAGACAGTGAA | |
DLG_exon19-F | ACGACAAGAGCAAGGACAGTGG | |
DLG_exon18-F | CCATGACCTACGAGAGCAGATGA | |
DLG_exon17-F | GGCTGGACAGACAGTGACGATT | |
DLG_exon16-F | GTGTCCTTCATTCTGGCTGGTG | |
DLG_exon15-F | ACATAGCACCGCAACTCGTCAG |