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01.04.2005 | Original Article

Distinction of acute lymphoblastic leukemia from acute myeloid leukemia through microarray-based DNA methylation analysis

verfasst von: Christian Scholz, Inko Nimmrich, Matthias Burger, Evelyne Becker, Bernd Dörken, Wolf-Dieter Ludwig, Sabine Maier

Erschienen in: Annals of Hematology | Ausgabe 4/2005

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Abstract

Patterns of DNA methylation are substantially altered in malignancies compared to normal tissue, with both genome-wide hypomethylation and regional increase of cytosine methylation at dinucleotides of cytosine and guanine, i.e., CpG dinucleotides. While genome-wide hypomethylation renders chromosomes instable, hypermethylation of CpGs in promoter regions is generally associated with transcriptional silencing, e.g., of tumor suppressor genes. To investigate whether disease-specific methylation profiles exist for different entities of acute leukemia, a microarray-based DNA methylation analysis simultaneously assessing 249 CpG dinucleotides originating from 57 genes was employed. Hereby, samples from precursor B-cell acute lymphoblastic leukemia (ALL) could be distinguished from cases of acute myeloid leukemia by virtue of N33, EGR4, CDC2, CCND2, or MOS hypermethylation in ALL.

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Titel: Distinction of acute lymphoblastic leukemia from acute myeloid leukemia through microarray-based DNA methylation analysis
verfasst von: Christian Scholz
Inko Nimmrich
Matthias Burger
Evelyne Becker
Bernd Dörken
Wolf-Dieter Ludwig
Sabine Maier
Publikationsdatum: 01.04.2005
Verlag: Springer-Verlag
Erschienen in: Annals of Hematology / Ausgabe 4/2005
Print ISSN: 0939-5555
Elektronische ISSN: 1432-0584
DOI: https://doi.org/10.1007/s00277-004-0969-1

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Distinction of acute lymphoblastic leukemia from acute myeloid leukemia through microarray-based DNA methylation analysis

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