Background
Methods
Material
Methods research protocols
Specimen collection
Determination of HPV genotype
Real time-PCR for HR-HPV DNA
Statistical analysis
Results
Distribution of HPV types in CIN and ICC women from the Yangtze River Delta region
Types | CIN1 | CIN2 | CIN3 | ICC | Total | |||||
---|---|---|---|---|---|---|---|---|---|---|
n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | |
Total | 751 | 425 | 753 | 252 | 2181 | |||||
Total HPV (+) | 666 | (88.68) | 392 | (92.24) | 701 | (93.09) | 222 | (88.10) | 1981 | (90.83) |
16
| 117 | (17.57) | 135 | (34.44) | 411 | (58.63) | 146 | 65.77) | 809 | (40.84) |
58
| 167 | (25.08) | 95 | (24.23) | 129 | (18.40) | 20 | (9.01) | 411 | (20.75) |
52
| 184 | (27.63) | 75 | (19.13) | 93 | (13.27) | 19 | (8.56) | 371 | (18.73) |
33
| 59 | (8.86) | 47 | (11.99) | 104 | (14.84) | 15 | (6.76) | 225 | (11.36) |
18
| 41 | (6.16) | 20 | (5.10) | 32 | (4.56) | 27 | (12.16) | 120 | (6.06) |
31
| 38 | (5.71) | 29 | (7.40) | 54 | (7.70) | 9 | (4.05) | 130 | (6.56) |
53
| 38 | (5.71) | 25 | (6.38) | 37 | (5.28) | 12 | (5.41) | 112 | (5.65) |
68
| 34 | (5.11) | 16 | (4.08) | 27 | (3.85) | 10 | (4.50) | 87 | (4.39) |
39
| 32 | (4.80) | 11 | (2.81) | 12 | (1.71) | 7 | (3.15) | 62 | (3.13) |
51
| 31 | (4.65) | 11 | (2.81) | 6 | (0.86) | 2 | (0.90) | 50 | (2.52) |
66
| 30 | (4.50) | 8 | (2.04) | 10 | (1.43) | 1 | (0.45) | 49 | (2.47) |
56
| 30 | (4.50) | 4 | (1.02) | 7 | (1.00) | 1 | (0.45) | 42 | (2.12) |
35
| 10 | (1.50) | 8 | (2.04) | 5 | (0.71) | 1 | (0.45) | 24 | (1.21) |
CP | 49 | (7.36) | 25 | (6.38) | 28 | (3.99) | 10 | (4.50) | 112 | (5.65) |
11 | 15 | (2.25) | 5 | (1.28) | 9 | (1.28) | 4 | (1.80) | 33 | (1.67) |
6 | 7 | (1.05) | 9 | (2.30) | 5 | (0.71) | 1 | (0.45) | 22 | (1.11) |
42 | 7 | (1.05) | 2 | (0.51) | 5 | (0.71) | 3 | (1.35) | 17 | (0.86) |
Frequency of infection with a single or multiple HPV types in the Yangtze River Delta region
Frequency of multiple HPV types in the three regions (Table 2)
Area | TTotal | Not detected (HPV-) | Types (%) of HPV(+) | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
n | (%) | Single type | Two types | Three types | Four types | Five types | Six types | Eight types | |||||||||
Zhejiang | 941 | 94 | (9.99) | 603 | (64.08) | 183 | (19.45) | 50 | (5.31) | 8 | (0.85) | 2 | (0.21) | 1 | (0.11) | 0 | (0.00) |
Jiangsu | 784 | 69 | (8.80) | 473 | (60.33) | 170 | (21.68) | 49 | (6.25) | 19 | (2.42) | 3 | (0.38) | 0 | (0.00) | 1 | (0.13) |
Shanghai | 456 | 37 | (8.11) | 296 | (64.91) | 102 | (22.37) | 19 | (4.17) | 2 | (0.44) | 0 | (0.00) | 0 | (0.00) | 0 | (0.00) |
Total | 2181 | 200 | (9.17) | 1372 | (62.91) | 454 | (20.82) | 119 | (5.46) | 29 | (1.33) | 5 | (0.23) | 1 | (0.05) | 1 | (0.05) |
Regional distribution of disease and age in the Yangtze River Delta region (Table 3)
Age (years) | Total | CIN1 | CIN2 | CIN3 | ICC | |||||
---|---|---|---|---|---|---|---|---|---|---|
n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | |
≤24 | 43 | (1.97) | 21 | (2.80) | 12 | (2.83) | 9 | (1.19) | 1 | (0.40) |
25–29 | 201 | (9.22) | 95 | (12.65) | 40 | (9.43) | 60 | (7.95) | 6 | (2.39) |
30–34 | 338 | (15.50) | 128 | (17.04) | 73 | (17.22) | 116 | (15.36) | 21 | (8.37) |
35–39 | 395 | (18.11) | 134 | (17.84) | 81 | (19.10) | 144 | (19.07) | 36 | (14.34) |
40–44 | 404 | (18.52) | 134 | (17.84) | 67 | (15.80) | 150 | (19.87) | 53 | (21.12) |
45–49 | 306 | (14.03) | 99 | (13.18) | 60 | (14.15) | 109 | (14.44) | 38 | (15.14) |
50–54 | 191 | (8.76) | 45 | (5.99) | 44 | (10.38) | 61 | (8.08) | 41 | (16.33) |
55–59 | 100 | (4.59) | 29 | (3.86) | 21 | (4.95) | 33 | (4.37) | 17 | (6.77) |
≥60 | 203 | (9.31) | 66 | (8.79)) | 26 | (6.13) | 73 | (9.67) | 38 | (15.14) |
Total | 2181 | (100) | 751 | (100) | 424 | (100) | 755 | (100) | 251 | (100) |
Age distribution of CIN and ICC in Zhejiang Province (Table 4)
Age (years) | Total | CIN1 | CIN2 | CIN3 | ICC | |||||
---|---|---|---|---|---|---|---|---|---|---|
n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | |
≤24 | 17 | (1.81) | 14 | (3.29) | 2 | (3.28) | 0 | (0.00) | 1 | (0.65) |
25–29 | 89 | (9.46) | 61 | (14.35) | 6 | (9.84) | 21 | (6.98) | 1 | (0.65) |
30–34 | 140 | (14.88) | 80 | (18.82) | 6 | (9.84) | 42 | (13.95) | 12 | (7.79) |
35–39 | 135 | (14.35) | 63 | (14.82) | 3 | (4.92) | 50 | (16.61) | 19 | (12.34) |
40–44 | 166 | (17.64) | 75 | (17.65) | 6 | (9.84) | 48 | (15.95) | 37 | (24.03) |
45–49 | 143 | (15.20) | 58 | (13.65) | 10 | (16.39) | 49 | (16.28) | 26 | (16.88) |
50–54 | 71 | (7.55) | 10 | (2.35) | 9 | (14.75) | 27 | (8.97) | 25 | (16.23) |
55–59 | 47 | (4.99) | 15 | (3.53) | 10 | (16.39) | 16 | (5.32) | 6 | (3.90) |
≥60 | 133 | (14.13) | 49 | (11.53) | 9 | (14.75) | 48 | (15.95) | 27 | (17.53) |
Total | 941 | (100) | 425 | (100) | 61 | (100) | 301 | (100) | 154 | (100) |
Age distribution of CIN and ICC patients in Jiangsu Province (Table 5)
Age (years) | Total | CIN1 | CIN2 | CIN3 | ICC | |||||
---|---|---|---|---|---|---|---|---|---|---|
n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | |
≤24 | 19 | (2.42) | 2 | (1.23) | 9 | (3.70) | 8 | (2.61) | 0 | (0.00) |
25–29 | 69 | (8.80) | 15 | (9.26) | 20 | (8.23) | 29 | (9.45) | 5 | (6.94) |
30–34 | 148 | (18.88) | 27 | (16.67) | 54 | (22.22) | 59 | (19.22) | 8 | (11.11) |
35–39 | 167 | (21.30) | 36 | (22.22) | 53 | (21.81) | 66 | (21.50) | 12 | (16.67) |
40–44 | 145 | (18.49) | 32 | (19.75) | 39 | (16.05) | 65 | (21.17) | 9 | (12.50) |
45–49 | 91 | (11.61) | 15 | (9.26) | 28 | (11.52) | 38 | (12.38) | 10 | (13.89) |
50–54 | 66 | (8.42) | 15 | (9.26) | 19 | (7.82) | 21 | (6.84) | 11 | (15.28) |
55–59 | 36 | (4.59) | 9 | (5.56) | 10 | (4.12) | 8 | (2.61) | 9 | (12.50) |
≥60 | 43 | (5.48) | 11 | (6.79) | 11 | (4.53) | 13 | (4.23) | 8 | (11.11) |
Total | 784 | (100) | 162 | (100) | 243 | (100) | 307 | (100) | 72 | (100) |
Age distribution of CIN and ICC patients in Shanghai (Table 6)
Age (years) | Total | CIN1 | CIN2 | CIN3 | ICC | |||||
---|---|---|---|---|---|---|---|---|---|---|
n | (%) | n | (%) | n | (%) | n | (%) | n | (%) | |
≤24 | 7 | (1.54) | 5 | (3.05) | 1 | (0.83) | 1 | (0.68) | 0 | (0.00) |
25–29 | 43 | (9.43) | 19 | (11.59) | 14 | (11.67) | 10 | (6.80) | 0 | (0.00) |
30–34 | 50 | (10.96) | 21 | (12.80) | 13 | (10.83) | 15 | (10.20) | 1 | (4.00) |
35–39 | 93 | (20.39) | 35 | (21.34) | 25 | (20.83) | 28 | (19.05) | 5 | (20.00) |
40–44 | 93 | (20.39) | 27 | (16.46) | 22 | (18.33) | 37 | (25.17) | 7 | (28.00) |
45–49 | 72 | (15.79) | 26 | (15.85) | 22 | (18.33) | 22 | (14.97) | 2 | (8.00) |
50–54 | 54 | (11.84) | 20 | (12.20) | 16 | (13.33) | 13 | (8.84) | 5 | (20.00) |
55–59 | 18 | (3.95) | 5 | (3.05) | 1 | (0.83) | 9 | (6.12) | 3 | (12.00) |
≥60 | 26 | (5.70) | 6 | (3.66) | 6 | (5.00) | 12 | (8.16) | 2 | (8.00) |
Total | 456 | (100) | 164 | (100) | 120 | (100) | 147 | (100) | 25 | (100) |