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Obesity Surgery
Erschienen in: Obesity Surgery 3/2016

01.03.2016 | Original Contributions

DNA Methylation and Hydroxymethylation Levels in Relation to Two Weight Loss Strategies: Energy-Restricted Diet or Bariatric Surgery

verfasst von: Carolina Ferreira Nicoletti, Carla Barbosa Nonino, Bruno Affonso Parenti de Oliveira, Marcela Augusta de Souza Pinhel, Maria Luisa Mansego, Fermin Ignacio Milagro, Maria Angeles Zulet, José Alfredo Martinez

Erschienen in: Obesity Surgery | Ausgabe 3/2016

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Abstract

Background

Weight loss can be influenced by genetic factors and epigenetic mechanisms that participate in the regulation of body weight. This study aimed to investigate whether the weight loss induced by two different obesity treatments (energy restriction or bariatric surgery) may affect global DNA methylation (LINE-1) and hydroxymethylation profile, as well as the methylation patterns in inflammatory genes.

Methods

This study encompassed women from three differents groups: 1. control group (n = 9), normal weight individuals; 2. energy restriction group (n = 22), obese patients following an energy-restricted Mediterranean-based dietary treatment (RESMENA); and 3. bariatric surgery group (n = 14), obese patients underwent a hypocaloric diet followed by bariatric surgery. Anthropometric measurements and 12-h fasting blood samples were collected before the interventions and after 6 months. Lipid and glucose biomarkers, global hydroxymethylation (by ELISA), LINE-1, SERPINE-1, and IL-6 (by MS-HRM) methylation levels were assessed in all participants.

Results

Baseline LINE-1 methylation was associated with serum glucose levels whereas baseline hydroxymethylation was associated with BMI, waist circumference, total cholesterol, and triglycerides. LINE-1 and SERPINE-1 methylation levels did not change after weight loss, whereas IL-6 methylation increased after energy restriction and decreased in the bariatric surgery group. An association between SERPINE-1 methylation and weight loss responses was found.

Conclusions

Global DNA methylation and hydroxymethylation might be biomarkers for obesity and associated comorbidities. Depending on the obesity treatment (diet or surgery), the DNA methylation patterns behave differently. Baseline SERPINE-1 methylation may be a predictor of weight loss values after bariatric surgery.
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Metadaten
Titel
DNA Methylation and Hydroxymethylation Levels in Relation to Two Weight Loss Strategies: Energy-Restricted Diet or Bariatric Surgery
verfasst von
Carolina Ferreira Nicoletti
Carla Barbosa Nonino
Bruno Affonso Parenti de Oliveira
Marcela Augusta de Souza Pinhel
Maria Luisa Mansego
Fermin Ignacio Milagro
Maria Angeles Zulet
José Alfredo Martinez
Publikationsdatum
01.03.2016
Verlag
Springer US
Erschienen in
Obesity Surgery / Ausgabe 3/2016
Print ISSN: 0960-8923
Elektronische ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-015-1802-8

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