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Erschienen in: Journal of Assisted Reproduction and Genetics 12/2010

01.12.2010 | Epigenetics

Effect of cryopreservation on acetylation patterns of lysine 12 of histone H4 (acH4K12) in mouse oocytes and zygotes

verfasst von: Lun Suo, QingGang Meng, Yan Pei, XiangWei Fu, YanPing Wang, Thomas D. Bunch, ShiEn Zhu

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 12/2010

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Abstract

Purpose

To determine the effect of cryopreservation on acH4K12 in oocytes and their respective zygotes.

Methods

AcH4K12 in fresh or vitrified-warmed oocytes and their respective zygotes at 70 min–12 h post-fertilization were assessed using fluorescent staining.

Results

1. AcH4K12 levels increased significantly in vitrified oocytes compared to controls. 2. Respective zygotes derived from vitrified oocytes had abnormal chromatin distribution or acH4K12 patterns before and after pronuclear formation.

Conclusion

Cryopreservation alters AcH4K12 patterns in oocytes, which subsequently affect the chromatin distribution and acH4K12 in fertilized oocytes.
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Metadaten
Titel
Effect of cryopreservation on acetylation patterns of lysine 12 of histone H4 (acH4K12) in mouse oocytes and zygotes
verfasst von
Lun Suo
QingGang Meng
Yan Pei
XiangWei Fu
YanPing Wang
Thomas D. Bunch
ShiEn Zhu
Publikationsdatum
01.12.2010
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 12/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-010-9469-5

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