Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende
Erweiterte Suche
Anmelden
nach oben
Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 12/2010

01.12.2010 | Stem Cell Biology

Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods

verfasst von: Sheyla González, Elena Ibáñez, Josep Santaló

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 12/2010

Einloggen, um Zugang zu erhalten

Abstract

Purpose

The aim of the present study is to compare three previously described mouse embryonic stem cell derivation methods to evaluate the influence of culture conditions, number of isolated blastomeres and embryonic stage in the derivation process.

Methods

Three embryonic stem cell derivation methods: standard, pre-adhesion and defined culture medium method, were compared in the derivation from isolated blastomeres and whole embryos at 4- and 8-cell stages.

Results

A total of 200 embryonic stem cell lines were obtained with an efficiency ranging from 1.9% to 72%.

Conclusions

Using either isolated blastomeres or whole embryos, the highest rates of mouse embryonic stem cell establishment were achieved with the defined culture medium method and efficiencies increased as development progressed. Using isolated blastomeres, efficiencies increased in parallel to the proportion of the embryo volume used to start the derivation process.
Literatur
1.
2.
Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981;292:154–6.CrossRefPubMed
3.
Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci. 1981;78:7634–8.CrossRefPubMed
4.
Williams RL, Hilton DJ, Pease S, Willson TA, Stewart CL, Gearing DP, et al. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature. 1988;336:684–7.CrossRefPubMed
5.
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282:1145–7.CrossRefPubMed
6.
Niwa H. Molecular mechanism for cell-fate determination in ES cells. Tanpakushitsu Kakusan Koso. 2000;45:2047–55. In Japanese.PubMed
7.
Eistetter HR. Pluripotent embryonal stem cell lines can be established from disaggregated mouse morulae. Develop Growth Differ. 1989;31:275–82.CrossRef
8.
Tesar PJ. Derivation of germ-line-competent embryonic stem cell lines from preblastocyst mouse embryos. Proc Natl Acad Sci. 2005;102:8239–44.CrossRefPubMed
9.
Strelchenko N, Verlinsky O, Kukharenko V, Verlinsky Y. Morula-derived human embryonic stem cells. Reprod Biomed Online. 2004;9:623–9.CrossRefPubMed
10.
Chung Y, Klimanskaya I, Becker S, Marh J, Lu SJ, Johnson J, et al. Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres. Nature. 2006;439:216–9.CrossRefPubMed
11.
Chung Y, Klimanskaya I, Becker S, Maserati M, Lu SJ, Zdravkovic T, et al. Human embryonic stem cell lines generated without embryo destruction. Cell Stem Cell. 2008;2:113–7.CrossRefPubMed
12.
Geens M, Mateizel I, Sermon K, De Rycke M, Spits C, Cauffman G, et al. Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos. Hum Reprod. 2009;24:2709–17.CrossRefPubMed
13.
Klimanskaya I, Chung Y, Becker S, Lu SJ, Lanza R. Human embryonic stem cell lines derived from single blastomeres. Nature. 2006;444:481–5.CrossRefPubMed
14.
Wakayama S, Hikichi T, Suetsugu R, Sakaide Y, Bui HT, Mizutani E, et al. Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies. Stem Cells. 2007;25:986–93.CrossRefPubMed
15.
Handyside AH, Lesko JG, Tarin JJ, Winston RM, Hughes MR. Birth of a normal girl after in vitro fertilization and preimplantation diagnostic testing for cystic fibrosis. N Engl J Med. 1992;327:905–9.CrossRefPubMed
16.
Veiga A, Calderon G, Barri PN, Coroleu B. Pregnancy after the replacement of a frozen-thawed embryo with less than 50% intact blastomeres. Hum Reprod. 1987;2:321–3.PubMed
17.
Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc Natl Acad Sci. 1975;72:1441–5.CrossRefPubMed
18.
Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc Natl Acad Sci. 1997;94:5709–12.CrossRefPubMed
19.
Biggers JD, McGinnis LK, Raffin M. Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium. Biol Reprod. 2000;63:281–93.CrossRefPubMed
20.
Delhaise F, Bralion V, Schuurbiers N, Dessy F. Establishment of an embryonic stem cell line from 8-cell stage mouse embryos. Eur J Morphol. 1996;34:237–43.CrossRefPubMed
21.
Robertson EJ. Embryo-derived stem cell lines. In: Robertson EJ, editor. Teratocarcinomas and embryonic stem cells a practical approach. Oxford: IRL; 1987. p. 71–112.
22.
Lanza R, Weissman I, Thomson J, Pedersen R, Hogan B, Gearhart J, et al. Isolation and maintenance of murine embryonic stem cells. New York: Cold Spring Harbor; 2004.
23.
Kawase E, Suemori H, Takahashi N, Okazaki K, Hashimoto K, Nakatsuji N. Strain difference in establishment of mouse embryonic stem (ES) cell lines. Int J Dev Biol. 1994;38:385–90.PubMed
24.
Willadsen SM. The viability of early cleavage stages containing half the normal number of blastomeres in the sheep. J Reprod Fertil. 1980;59:357–62.CrossRefPubMed
25.
Willadsen SM. The development capacity of blastomeres from 4- and 8-cell sheep embryos. J Embryol Exp Morphol. 1981;65:165–72.PubMed
26.
Tarkowski AK, Ozdzenski W, Czolowska R. Identical triplets and twins developed from isolated blastomeres of 8- and 16-cell mouse embryos supported with tetraploid blastomeres. Int J Dev Biol. 2005;49:825–32.CrossRefPubMed
27.
Steinberg MS, McNutt PM. Cadherins and their connections: adhesion junctions have broader functions. Curr Opin Cell Biol. 1999;11:554–60.CrossRefPubMed
28.
29.
Lorthongpanich C, Yang SH, Piotrowska-Nitsche K, Parnpai R, Chan AW. Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells. Reproduction. 2008;135:805–13.CrossRefPubMed
30.
Gardner RL. Specification of embryonic axes begins before cleavage in normal mouse development. Development. 2001;128:839–47.PubMed
31.
Fujimori T, Kurotaki Y, Miyazaki J, Nabeshima Y. Analysis of cell lineage in two- and four-cell mouse embryos. Development. 2003;130:5113–22.CrossRefPubMed
32.
Piotrowska-Nitsche K, Zernicka-Goetz M. Spatial arrangement of individual 4-cell stage blastomeres and the order in which they are generated correlate with blastocyst pattern in the mouse embryo. Mech Dev. 2005;122:487–500.CrossRefPubMed
33.
Tarkowski AK, Wroblewska J. Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage. J Embryol Exp Morphol. 1967;18:155–80.PubMed
34.
Rossant J. Postimplantation development of blastomeres isolated from 4- and 8-cell mouse eggs. J Embryol Exp Morphol. 1976;36:283–90.PubMed
Metadaten
Titel
Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods
verfasst von
Sheyla González
Elena Ibáñez
Josep Santaló
Publikationsdatum
01.12.2010
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 12/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-010-9473-9

Weitere Artikel der Ausgabe 12/2010

Journal of Assisted Reproduction and Genetics 12/2010 Zur Ausgabe

Assisted Reproduction Technologies

Comparison of early pregnancy and neonatal outcomes after frozen and fresh embryo transfer in ART cycles

Genetics

The utility of embryo banking in order to increase the number of embryos available for preimplantation genetic screening in advanced maternal age patients

Gamete Biology

Analysis and difference of voltage-dependent anion channel mRNA in ejaculated spermatozoa from normozoospermic fertile donors and infertile patients with idiopathic asthenozoospermia

Assisted Reproduction Technologies

The role of the Hoxa10/HOXA10 gene in the etiology of endometriosis and its related infertility: a review

Genetics

Length difference between equine ZFX and ZFY genes and its application for molecular sex determination

Commentary

Deconstructing the promise of embryonic stem-cell research

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hirsutismus bei PCOS: Laser- und Lichttherapien helfen

26.04.2024 Hirsutismus Nachrichten

Laser- und Lichtbehandlungen können bei Frauen mit polyzystischem Ovarialsyndrom (PCOS) den übermäßigen Haarwuchs verringern und das Wohlbefinden verbessern – bei alleiniger Anwendung oder in Kombination mit Medikamenten.

ICI-Therapie in der Schwangerschaft wird gut toleriert

23.04.2024 Medikamente in Schwangerschaft und Stillzeit Nachrichten

Müssen sich Schwangere einer Krebstherapie unterziehen, rufen Immuncheckpointinhibitoren offenbar nicht mehr unerwünschte Wirkungen hervor als andere Mittel gegen Krebs.

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

23.04.2024 Depression antepartal oder postpartal Nachrichten

Bislang gibt es kein Medikament zur Prävention von Wochenbettdepressionen. Das Injektionsanästhetikum Esketamin könnte womöglich diese Lücke füllen.

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

22.04.2024 DGIM 2024 Kongressbericht

Durch die Häufung nach der COVID-19-Pandemie sind Infektionen mit dem Respiratorischen Synzytial-Virus (RSV) in den Fokus gerückt. Fachgesellschaften empfehlen eine Impfung inzwischen nicht nur für Säuglinge und Kleinkinder.

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen
Bildnachweise