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Erschienen in:

01.12.2007 | Original Article

Effect of dehydroepiandrosterone on insulin sensitivity in Otsuka Long-Evans Tokushima-fatty rats

verfasst von: Tatsuo Ishizuka, Atsushi Miura, Kazuo Kajita, Masami Matsumoto, Chiyo Sugiyama, Kenji Matsubara, Takahide Ikeda, Ichiro Mori, Hiroyuki Morita, Yoshihiro Uno, Tomoatsu Mune, Yoshinori Kanoh, Masayoshi Ishizawa

Erschienen in: Acta Diabetologica | Ausgabe 4/2007

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Abstract

In order to clarify the effect of dehydroepiandrosterone (DHEA) on improvement of insulin resistance, we examined the effects of overexpression of wild-type protein kinase C-ζ (wt-PKCζ)/3-phosphoinositide-dependent protein kinase-1 (wt-PDK1) and kinase-inactive PKCζ/PDK1 (ΔPKCζ/ΔPDK1) on DHEA-induced [³H]2-deoxyglucose (DOG) uptake using the electroporation method in rat adipocytes. Overexpression of wt-PKCζ and wt-PDK1 significantly increased in DHEA-induced [³H]2-DOG uptake. Wortmannin completely suppressed DHEA-induced [³H]2-DOG uptake in wt-PKCζ- and wt-PDK1-transfected adipocytes. Overexpression of neither ΔPKCζ nor ΔPDK1 increased DHEA-induced [³H]2-DOG uptake. Otsuka Long-Evans fatty rats (OLETF), animal models of type 2 diabetes, and Long-Evans Tokushima rats (LETO) as control, were treated with 0.4% DHEA for 2 weeks. Insulin-induced [³H]2-DOG uptakes, activations of PI 3-kinase and PKCζ of adipocytes were significantly increased in DHEA-treated OLETF rats. Moreover, plasma glucose levels in OLETF rats after treatment with DHEA for 2 weeks were significantly lower than treatment without DHEA, but not in LETO rats. These results indicate that DHEA treatment may improve glucose tolerance through a PI 3-kinase-PKCζ pathway and downregulates adiposity in OLETF rats.

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Titel: Effect of dehydroepiandrosterone on insulin sensitivity in Otsuka Long-Evans Tokushima-fatty rats
verfasst von: Tatsuo Ishizuka
Atsushi Miura
Kazuo Kajita
Masami Matsumoto
Chiyo Sugiyama
Kenji Matsubara
Takahide Ikeda
Ichiro Mori
Hiroyuki Morita
Yoshihiro Uno
Tomoatsu Mune
Yoshinori Kanoh
Masayoshi Ishizawa
Publikationsdatum: 01.12.2007
Verlag: Springer Milan
Erschienen in: Acta Diabetologica / Ausgabe 4/2007
Print ISSN: 0940-5429
Elektronische ISSN: 1432-5233
DOI: https://doi.org/10.1007/s00592-007-0009-4

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