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01.04.2011 | Original Article

Effect of lemon verbena supplementation on muscular damage markers, proinflammatory cytokines release and neutrophils’ oxidative stress in chronic exercise

verfasst von: Lorena Funes, Lucrecia Carrera-Quintanar, Manuela Cerdán-Calero, Miguel D. Ferrer, Franchek Drobnic, Antoni Pons, Enrique Roche, Vicente Micol

Erschienen in: European Journal of Applied Physiology | Ausgabe 4/2011

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Abstract

Intense exercise is directly related to muscular damage and oxidative stress due to excessive reactive oxygen species (ROS) in both, plasma and white blood cells. Nevertheless, exercise-derived ROS are essential to regulate cellular adaptation to exercise. Studies on antioxidant supplements have provided controversial results. The purpose of this study was to determine the effect of moderate antioxidant supplementation (lemon verbena extract) in healthy male volunteers that followed a 90-min running eccentric exercise protocol for 21 days. Antioxidant enzymes activities and oxidative stress markers were measured in neutrophils. Besides, inflammatory cytokines and muscular damage were determined in whole blood and serum samples, respectively. Intense running exercise for 21 days induced antioxidant response in neutrophils of trained male through the increase of the antioxidant enzymes catalase, glutathione peroxidase and glutathione reductase. Supplementation with moderate levels of an antioxidant lemon verbena extract did not block this cellular adaptive response and also reduced exercise-induced oxidative damage of proteins and lipids in neutrophils and decreased myeloperoxidase activity. Moreover, lemon verbena supplementation maintained or decreased the level of serum transaminases activity indicating a protection of muscular tissue. Exercise induced a decrease of interleukin-6 and interleukin-1β levels after 21 days measured in basal conditions, which was not inhibited by antioxidant supplementation. Therefore, moderate antioxidant supplementation with lemon verbena extract protects neutrophils against oxidative damage, decreases the signs of muscular damage in chronic running exercise without blocking the cellular adaptation to exercise.

Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126PubMedCrossRef

Baum M, Köpping-Menke K et al (1999) Increased concentrations of interleukin 1-beta in whole blood cultures supernatants after 12 weeks of moderate endurance exercise. Eur J Appl Physiol Occup Physiol 79(6):500–503PubMedCrossRef

Davison G, Gleeson M et al (2007) Antioxidant supplementation and immunoendocrine responses to prolonged exercise. Med Sci Sports Exerc 39(4):645–652PubMedCrossRef

Díaz AM, Abad MJ et al (2004) Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro anti-inflammatory activity. Life Sci 74(20):2515–2526PubMedCrossRef

Fallon KE, Sivyer G et al (1999) The biochemistry of runners in a 1600 km ultramarathon. Br J Sport Med 33(4):264–269CrossRef

Ferrer MD, Tauler P et al (2009) Antioxidant regulatory mechanisms in neutrophils and lymphocytes after intense exercise. J Sports Sci 27(1):49–58PubMedCrossRef

Fischer CP, Hiscock NJ et al (2004) Supplementation with vitamins C and E inhibits the release of interleukin-6 from contracting human skeletal muscle. J Physiol 558(2):633–645PubMedCrossRef

Flohe L, Gunzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105:114–121PubMedCrossRef

Funes L, Fernández-Arroyo S et al (2009) Correlation between plasma antioxidant capacity and verbascoside levels in rats after oral administration of lemon verbena extract. Food Chem 117(4):589–598CrossRef

Gleeson M (2007) Immune function in sport and exercise. J Appl Physiol 103(2):693–699PubMedCrossRef

Goldberg DM, Spooner RJ (1983) Glutathione reductase. In: Bergmeyer HU, Bergmeyer J, Grabl M (eds) Methods of enzymatic analysis. Verlag Chemie, Basal, pp 258–265

Gómez-Cabrera M-C, Domenech E et al (2008) Oral administration of vitamin C decreases muscle mitochondrial biogenesis and hampers training-induced adaptations in endurance performance. Am J Clin Nutr 87(1):142–149PubMed

Hausmann M, Obermeier F et al (2007) In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis. Clin Exp Immunol 148(2):373–381PubMedCrossRef

Ji LL (1995) Oxidative stress during exercise: implication of antioxidant nutrients. Free Radic Biol Med 18:1079–1086PubMedCrossRef

Ji LL (2008) Modulation of skeletal muscle antioxidant defense by exercise: role of redox signaling. Free Radic Biol Med 44(2):142–152PubMedCrossRef

Ji LL, Gómez-Cabrera MC et al (2004) Acute exercise activates nuclear factor (NF)-kappaB signaling pathway in rat skeletal muscle. Faseb J 18:1499–1506PubMedCrossRef

Kim H, Lee Y et al (2007) Biomarkers of muscle and cartilage damage and inflammation during a 200 km run. Eur J Appl Physiol 99(4):443–447PubMedCrossRef

Korkina LG, Mikhal’chik EV et al (2007) Molecular mechanisms underlying wound healing and anti-inflammatory properties of naturally occurring biotechnologically produced phenylpropanoid glycosides. Cell Mol Biol 53(5):84–91PubMed

Lee JY, Woo ER et al (2005) Inhibition of lipopolysaccharide-inducible nitric oxide synthase expression by acteoside through blocking of AP-1 activation. J Ethnopharmacol 97(3):561–566PubMedCrossRef

Levine RL, Williams JA et al (1994) Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol 233:346–357PubMedCrossRef

Lin L-C, Wang Y-H et al (2006) The inhibitory effect of phenylpropanoid glycosides and iridoid glucosides on free radical production and beta2 integrin expression in human leucocytes. J Pharm Pharmacol 58(1):129–135PubMedCrossRef

Liu MJ, Li JX et al (2003) The effects of verbascoside on plasma lipid peroxidation level and erythrocyte membrane fluidity during immobilization in rabbits: a time course study. Life Sci 73(7):883–892PubMedCrossRef

McAnulty SR, McAnulty LS et al (2004) Consumption of blueberry polyphenols reduces exercise-induced oxidative stress compared to vitamin C. Nutr Res 24(3):209–221CrossRef

McCord JM, Fridovich I (1969) Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244(22):6049–6055PubMed

Miura M, Umeda T et al (2005) Effect of 6 months’ training on the reactive oxygen species production capacity of neutrophils and serum opsonic activity in judoists. Luminescence 20(1):1–7PubMedCrossRef

Morillas-Ruiz J, Zafrilla P et al (2005) The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95(5–6):543–549PubMedCrossRef

Morillas-Ruiz JM, Villegas García JA et al (2006) Effects of polyphenolic antioxidants on exercise-induced oxidative stress. Clin Nutr 25(3):444–453PubMedCrossRef

Morozov VI, Pryatkin SA et al (2003) Effect of exercise to exhaustion on myeloperoxidase and lysozyme release from blood neutrophils. Eur J Appl Physiol 89(3–4):257–262PubMedCrossRef

Morozov VI, Tsyplenkov PV et al (2006) The effects of high-intensity exercise on skeletal muscle neutrophil myeloperoxidase in untrained and trained rats. Eur J Appl Physiol 97(6):716–722PubMedCrossRef

Newall CA, Anderson LA, Phillipson JD (1996) Herbal medicines: a guide for health-care professionals. The Pharmaceutical Press, London, p 263

Nieman D (1994) Exercise, upper respiratory tract infection, and the immune system. Med Sci Sports Exerc 26:128–139PubMedCrossRef

Nieman DC, Henson DA et al (2007) Quercetin’s influence on exercise-induced changes in plasma cytokines and muscle and leukocyte cytokine mRNA. J Appl Physiol 103(5):1728–1735PubMedCrossRef

Noakes TD (1987) Effect of exercise on serum enzyme activities in humans. Sports Med 4(4):245–267PubMedCrossRef

Panza VSP, Wazlawik E et al (2008) Consumption of green tea favorably affects oxidative stress markers in weight-trained men. Nutrition 24(5):433–442PubMedCrossRef

Pedersen BK, Hoffman-Goetz L (2000) Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev Suppl 80(3):1055–1081

Pedersen BK, Toft AD (2000) Effects of exercise on lymphocytes and cytokines. Br J Sport Med 34(4):246–251CrossRef

Petersen EW, Ostrowski K et al (2001) Effect of vitamin supplementation on cytokine response and on muscle damage after strenuous exercise. Am J Physiol Cell Physiol 280:C1570–C1575PubMed

Phillips T, Childs AC et al (2003) A dietary supplement attenuates IL-6 and CRP after eccentric exercise in untrained males. Med Sci Sports Exerc 35(12):2032–2037PubMedCrossRef

Quirantes-Piné R, Funes L et al (2009) High-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight and ion-trap tandem mass spectrometry to identify phenolic compounds from a lemon verbena extract. J Chromatogr A 1216(28):5391–5397PubMedCrossRef

Ristow M, Zarse K et al (2009) Antioxidants prevent health-promoting effects of physical exercise in humans. Proc Natl Acad Sci USA 106(21):8665–8670PubMedCrossRef

Su Q-S, Tian Y et al (2008) Effects of allicin supplementation on plasma markers of exercise-induced muscle damage, IL-6 and antioxidant capacity. Eur J Appl Physiol 103(3):275–283PubMedCrossRef

Sureda A, Batle JM et al (2004a) Hypoxia/reoxygenation and vitamin C intake influence no synthesis and antioxidant defenses of neutrophils. Free Radic Biol Med 37(11):1744–1755PubMedCrossRef

Sureda A, Batle JM et al (2004b) Neutrophil tolerance to oxidative stress induced by hypoxia/reoxygenation. Free Radic Res 38(9):1003–1009PubMedCrossRef

Sureda A, Tauler P et al (2005) Relation between oxidative stress markers and antioxidant endogenous defences during exhaustive exercise. Free Radic Res 39(12):1317–1324PubMedCrossRef

Sureda A, Ferrer MD et al (2007a) Intense physical activity enhances neutrophil antioxidant enzyme gene expression. Immunocytochemistry evidence for catalase secretion. Free Radic Res 41(8):874–883PubMedCrossRef

Sureda A, Tauler P et al (2007b) Antioxidant supplementation influences the neutrophil tocopherol associated protein expression, but not the inflammatory response to exercise. Cent Eur J Biol 2(1):56–70CrossRef

Sureda A, Tauler P et al (2008) Influence of an antioxidant vitamin-enriched drink on pre- and post-exercise lymphocyte antioxidant system. Ann Nutr Metab 52:233–240PubMedCrossRef

Sureda A, Ferrer MD et al (2009) Effects of exercise intensity on lymphocyte H₂O₂ pro-duction and antioxidant defences in soccer players. Br J Sport Med 43:186–190CrossRef

Suzuki K, Totsuka M et al (1999) Endurance exercise causes interaction among stress hormones, cytokines, neutrophil dynamics, and muscle damage. J Appl Physiol 87(4):1360–1367PubMed

Tauler P, Aguiló A et al (2002a) Acute phase immune response to exercise coexists with decreased neutrophil antioxidant enzyme defences. Free Radic Res 36(10):1101–1107PubMedCrossRef

Tauler P, Aguiló A et al (2002b) Diet supplementation with vitamin E, vitamin C and beta-carotene cocktail enhances basal neutrophil antioxidant enzymes in athletes. Pflugers Arch 443(5–6):791–797PubMed

Tauler P, Aguiló A et al (2004) Different effects of exercise tests on the antioxidant enzyme activities in lymphocytes and neutrophils. J Nutr Biochem 15(8):479–484PubMedCrossRef

Tauler P, Sureda A et al (2006) Increased lymphocyte antioxidant defences in response to exhaustive exercise do not prevent oxidative damage. J Nutr Biochem 17(10):665–671PubMedCrossRef

Tauler P, Ferrer MD et al (2008) Supplementation with an antioxidant cocktail containing coenzyme Q prevents plasma oxidative damage induced by soccer. Eur J Appl Physiol 104(5):777–785PubMedCrossRef

Urso ML, Clarkson PM (2003) Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189(1–2):41–54PubMedCrossRef

Valentao P, Fernandes E et al (2002) Studies on the antioxidant activity of Lippia citriodora infusion: scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. Biol Pharm Bull 25(10):1324–1327PubMedCrossRef

Vassilakopoulos T, Karatza MH et al (2003) Antioxidants attenuate the plasma cytokine response to exercise in humans. J Appl Physiol 94(3):1025–1032PubMed

Yamada M, Suzuki K et al (2000) Effect of exhaustive exercise on human neutrophils in athletes. Luminescence 15(1):15–20PubMedCrossRef

Titel: Effect of lemon verbena supplementation on muscular damage markers, proinflammatory cytokines release and neutrophils’ oxidative stress in chronic exercise
verfasst von: Lorena Funes
Lucrecia Carrera-Quintanar
Manuela Cerdán-Calero
Miguel D. Ferrer
Franchek Drobnic
Antoni Pons
Enrique Roche
Vicente Micol
Publikationsdatum: 01.04.2011
Verlag: Springer-Verlag
Erschienen in: European Journal of Applied Physiology / Ausgabe 4/2011
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-010-1684-3

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