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01.01.2015 | Original Article

Effect of low-level laser therapy on the gene expression of collagen and vascular endothelial growth factor in a culture of fibroblast cells in mice

verfasst von: C. C. S. Martignago, R. F. Oliveira, D. A. A. Pires-Oliveira, P. D. Oliveira, C. Pacheco Soares, P. S. Monzani, R. C. Poli-Frederico

Erschienen in: Lasers in Medical Science | Ausgabe 1/2015

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Abstract

Low-level laser therapy treatment (LLLT) is widely used in rehabilitation clinics with the aim of accelerating the process of tissue repair; however, the molecular bases of the effect of LLLT have not been fully established. The aim of the present study was to evaluate the influence of the exposure of different doses of LLLT on the expression of collagen genes type I alpha 1 (COL1α1) and vascular endothelial growth factor (VEGF) in the fibroblast cells of mice (L929) cultivated in vitro. Fibroblast cells were irradiated with a Gallium-Arsenide laser (904 nm) every 24 h for 2 consecutive days, stored in an oven at 37 °C, with 5 % CO₂ and divided into 3 groups: G1—control group, G2—irradiated at 2 J/cm², and G3—irradiated at 3 J/cm². After irradiation, the total RNA was extracted and used in the complementary DNA (cDNA) synthesis. The gene expression was analyzed by real-time polymerase chain reaction. The cells irradiated in G2 exhibited a statistically significant growth of 1.78 in the expression of the messenger RNA (mRNA) of the COL1α1 gene (p = 0.036) in comparison with G1 and G3. As for the VEGF gene, an increase in expression was observed in the two irradiated groups in comparison with the control group. There was an increase in expression in G2 of 2.054 and G3 of 2.562 (p = 0.037) for this gene. LLLT (904 nm) had an influence on the expression of the genes COL1α1 (2 J/cm²) and VEGF (2 e 3 J/cm²) in a culture of the fibroblast cells of mice.

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Titel: Effect of low-level laser therapy on the gene expression of collagen and vascular endothelial growth factor in a culture of fibroblast cells in mice
verfasst von: C. C. S. Martignago
R. F. Oliveira
D. A. A. Pires-Oliveira
P. D. Oliveira
C. Pacheco Soares
P. S. Monzani
R. C. Poli-Frederico
Publikationsdatum: 01.01.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 1/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1644-y

Springer Medizin

Abstract

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