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International Journal of Diabetes in Developing Countries

Erschienen in:

01.03.2014 | Original Article

Effect of topical nitric oxide donor (0.2 % glyceryl trinitrate) on wound healing in diabetic wistar rats

verfasst von: V. Ghori, Divyesh R. Mandavia, Tejas K. Patel, C. B. Tripathi

Erschienen in: International Journal of Diabetes in Developing Countries | Ausgabe 1/2014

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Abstract

Cutaneous wound healing activity of 0.2 % glyceryl trinitrate (GTN) ointment was evaluated in diabetic wistar rats. Streptozotocin (STZ)-induced diabetic rats were divided in four groups (N = 6) and anaesthetized by ketamine 75 mg/kg and xylazine 10 mg/kg. Two groups were operated for excision wound (EW). One group served as control and another as 0.2 % GTN ointment treatment group. EW groups were studied for wound closure rate and re-epithelization. Remaining two groups were divided into control and treatment group. Rats in these two groups were inflicted with incision wound (IW) and dead space wound (DW) in the same animal. IWs were studied for wound tensile strength while DWs were studied for granulation tissue weight, hydroxyproline content and histological changes in granulation tissue. EW showed significantly higher re-epithelization in treatment group compared to control (61.10 ± 2.98 vs 27.36 ± 6.68; P = 0.01) but there was no difference in wound closure rate between the groups. 0.2 % GTN did not affect tensile strength of IW, granulation tissue weight or hydroxyproline content. This suggests that topical NO does not have any effect on collagen secretion and maturation by fibroblasts in incisional wound model. In conclusion, NO donor may useful in the wounds where wound contraction has a minor role in wound closure e.g., diabetic foot ulcers.

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Titel: Effect of topical nitric oxide donor (0.2 % glyceryl trinitrate) on wound healing in diabetic wistar rats
verfasst von: V. Ghori
Divyesh R. Mandavia
Tejas K. Patel
C. B. Tripathi
Publikationsdatum: 01.03.2014
Verlag: Springer India
Erschienen in: International Journal of Diabetes in Developing Countries / Ausgabe 1/2014
Print ISSN: 0973-3930
Elektronische ISSN: 1998-3832
DOI: https://doi.org/10.1007/s13410-013-0138-y

Springer Medizin

Abstract

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