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01.12.2012 | Original investigation | Ausgabe 1/2012 Open Access

Cardiovascular Diabetology 1/2012

Effect of vitamin D on aortic remodeling in streptozotocin-induced diabetes

Zeitschrift:
Cardiovascular Diabetology > Ausgabe 1/2012
Autoren:
Erik Salum, Priit Kampus, Mihkel Zilmer, Jaan Eha, Mark Butlin, Alberto P Avolio, Taavi Põdramägi, Andres Arend, Marina Aunapuu, Jaak Kals
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2840-11-58) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

ES performed the experiments, drafted the manuscript, and performed the statistical analysis. TP participated in designing the study and performing the experiments. ES, PK, JK, MZ, JE conceived of the study, participated in its design and coordination, and helped to draft the manuscript. MB and APA analysed the data, participated in the study design and coordination, and helped to draft the manuscript. MA and AA performed the histological analyses and helped to draft the manuscript. All authors have read and approved the final manuscript.

Abstract

Background

Diabetes mellitus is associated with micro- and macrovascular complications and increased cardiovascular risk. Elevated levels of serum asymmetric dimethylarginine (ADMA) may be responsible for endothelial dysfunction associated with diabetes-induced vascular impairment. Vitamin D may have potential protective effects against arterial stiffening. This study aimed to examine both the effects of diabetes on the functional/structural properties of the aorta and the endothelial function and the effects of vitamin D supplementation.

Methods

Male Wistar rats (n = 30) were randomly assigned to control untreated, diabetic untreated, and diabetic + cholecalciferol groups. Diabetes was induced by intraperitoneal injection of streptozotocin, followed by oral administration of cholecalciferol (500 IU/kg) for 10 weeks in the treatment group. Aortic pulse wave velocity (PWV) was recorded over a mean arterial pressure (MAP) range of 50 to 200 mmHg using a dual pressure sensor catheter. Intravenous infusion of phenylephrine and nitroglycerine was used to increase and decrease MAP, respectively. Serum 25-hydroxyvitamin D [25(OH)D] levels were measured using a radioimmune assay. ADMA levels in serum were measured by enzyme-linked immunoassay. Aortic samples were collected for histomorphometrical analysis.

Results

PWV up to MAP 170 mmHg did not reveal any significant differences between all groups, but in diabetic rats, PWV was significantly elevated across MAP range between 170 and 200 mmHg. Isobaric PWV was similar between the treated and untreated diabetic groups, despite significant differences in the levels of serum 25(OH)D (493 ± 125 nmol/L vs 108 ± 38 nmol/L, respectively). Serum levels of ADMA were similarly increased in the treated and untreated diabetic groups, compared to the control group. The concentration and integrity of the elastic lamellae in the medial layer of the aorta was impaired in untreated diabetic rats and improved by vitamin D supplementation.

Conclusion

PWV profile determined under isobaric conditions demonstrated differential effects of uncontrolled diabetes on aortic stiffness. Diabetes was also associated with elevated serum levels of ADMA. Vitamin D supplementation did not improve the functional indices of aortic stiffness or endothelial function, but prevented the fragmentation of elastic fibers in the aortic media.
Zusatzmaterial
Authors’ original file for figure 1
12933_2012_471_MOESM1_ESM.pdf
Authors’ original file for figure 2
12933_2012_471_MOESM2_ESM.jpeg
Authors’ original file for figure 3
12933_2012_471_MOESM3_ESM.jpeg
Literatur
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