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Acta Diabetologica

Erschienen in:

01.12.2010 | Original Article

Effects of intermittent high glucose on oxidative stress in endothelial cells

verfasst von: Qin-Min Ge, Yan Dong, Hong-Mei Zhang, Qing Su

Erschienen in: Acta Diabetologica | Sonderheft 1/2010

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Abstract

The objective of this study is to explore the mechanism of oxidative stress induced by intermittent high glucose in porcine iliac endothelial cells (PIECs). The PIECs were exposed to intermittent or constant high glucose for 3 or 6 days, and the mean fluorescent intensity (MFI) was measured via intracellular reactive oxygen species (ROS) captured by flow cytometry. The NADPH oxidase activity was measured by chemiluminescence with lucigenin. Intermittent high glucose induced a greater over-production of ROS than constant high glucose in PIECs; the NADPH oxidase activity was increased under both constant and intermittent high glucose conditions, being more marked in the latter (P < 0.05). In conclusion, intermittent high glucose induced more ROS in PIECs than constant high glucose, this effect seemed to be, at least in part related to the enhanced activation of NADPH oxidase. Glucose fluctuation may be involved in the development of vascular complications.

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Titel: Effects of intermittent high glucose on oxidative stress in endothelial cells
verfasst von: Qin-Min Ge
Yan Dong
Hong-Mei Zhang
Qing Su
Publikationsdatum: 01.12.2010
Verlag: Springer Milan
Erschienen in: Acta Diabetologica / Ausgabe Sonderheft 1/2010
Print ISSN: 0940-5429
Elektronische ISSN: 1432-5233
DOI: https://doi.org/10.1007/s00592-009-0140-5

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