nach oben

Comparative Clinical Pathology

Erschienen in:

01.11.2015 | Original Article

Effects of synbiotic (Biomin IMBO) on haematological and immunological components of Russian sturgeon, Acipenser guldenstadti

verfasst von: Ebrahim Jafarzadeh, Hossein Khara, Mohaddeseh Ahmadnezhad

Erschienen in: Comparative Clinical Pathology | Ausgabe 6/2015

Einloggen, um Zugang zu erhalten

Abstract

The present study was carried out to evaluate the effects of synbiotic (Biomin IMBO) on haematological (white blood cell (WBC) and red blood cell (RBC) counts, haemoglobin (Hb) concentration, haematocrit (Hct), mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV) and mean corpuscular haemoglobin concentration (MCHC)) and immunological (immunoglobulin (IgM) and lysozyme activity) components of Russian sturgeon, Acipenser guldenstadti. Five experimental treatments and one control group with three replicates were considered. In treatment groups, fish were fed with diet containing 1 (T₁), 1.5 (T₂), 2 (T₃), 2.5 (T₄) and 3 (T₅) g synbiotic/kg diet. In control group, fish were fed without supplement of synbiotic. Blood sampling for haematological and immunological studies was done at days 20, 40 and 60 after the beginning of the experiment. Based on our results, synbiotic supplement had no significant effects on values Hb, Htc, MCV and MCHC compared to control group (P > 0.05). Also, in all experimental treatments, these parameters did not considerable changes during the course of the experiment (P > 0.05). The effect of synbiotic supplement on MCH was not significant (P > 0.05); however, this value was higher in synbiotic supplement treatments compared to control group. MCH had no significant changes during the period of the experiment in all experimental groups (P > 0.05). In all synbiotic treatments, the values of WBC count, neutrophils, monocytes and eosinophils were higher than control group (P < 0.05). Only, lymphocytes in synbiotic supplement treatments were lower compared to control group (P < 0.05). Also, WBC count showed a decreasing trend during the period of the experiment in all synbiotic supplement groups (P < 0.05). Synbiotic supplement increased RBCs compared to control group; however, this was not significant (P > 0.05). The values of RBCs in T4 and T5 showed an increasing change over the course of the experiment (P < 0.05). The immunological components, i.e. IgM concentration and lysozyme activity, were found to be higher in synbiotic supplement treatments compared to control group (P < 0.05). These parameters had no significant changes over the course of the experiment (P > 0.05).

Aldrin JF, Messager JL, Laurencin FB (1982) La biochimie clinique en aquaculture. Interet et perspect CNEXO Actes Colloq 14:291–326

Al-Dohail MA, Hashim R, Aliyu-Paiko M (2009) Effects of the probiotic, Lactobacillus acidophilus, on the growth performance, haematology parameters and immunoglobulin concentration in African Catfish (Clarias gariepinus, Burchell 1822) fingerling. Aquacult Res 40:1642–1652CrossRef

Assem SS, El-Zaeem SY (2005) Application of biotechnology in ¢sh breeding II: production of highly immune genetically modified red belly tilapia, Tilapia zillii. Afr J of Biotechnol 4:449–459

Ayoola SO, Ajani EK, Fashae OF (2013) Effect of probiotics (Lactobacillus and Bifidobacterium) on growth performance and hematological profile of Clarias gariepinus juveniles. World J of Fish and Mar Sci 5:01–08

Blaxhall PC, Daisley KW (1973) Routine hematological methods for use with fish blood. J Fish Biol 5:771–781CrossRef

Cerezuela R, Meseguer J, Esteban MA (2011) Current knowledge in synbiotic use for fish aquaculture: a review. Aquac Res Dev. S, 1, p. 008

Chou TC, Rideout D, Chou J, Bertino JR (1991) Chemotherapeutic synergism, potentiation and antagonism. In: Dulbecco R (ed) Encyclopedia of human biology, vol 2. Academic Press, San Diego, California, pp 371–379

Dahiya T, Sihag RC, Gahlawat SK (2012) Effect of probiotics on the haematological parameters of Indian Magur (Clarius batrachus L.). J Fish Aqua Sci 7:279–290CrossRef

Dimitroglou A, Merrifeld DL, Carnevali O, Picchietti S, Avella M, Daniels C, Güroy D, Davies SJ (2011) Microbial manipulations to improve fsh health and production—a Mediterranean perspective. Fish Shellfish Immunol 30:1–16CrossRefPubMed

Drabkin DR (1945) Crystallographic and optical properties of human hemoglobin. A proposal for the standardisation of hemoglobin. Am J Med Sci 209:268–270

Ellis AE (1990) Lysozyme assays. In: Stolen JS, Fletcher TC, Anderson DP, Roberson BS, Muiswinkel WB (eds) Techniques in fish immunology. SOS publications, USA, pp 101–103

Fuller R (1989) Probiotics in man and animals. J Appl Bacteriol 66:365–378CrossRefPubMed

Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412PubMed

Hagi T, Hoshino T (2009) Screening and characterization of potential probiotic lactic acid bacteria from cultured common carp intestine. Biosci Biotechnol 73:1479–1483CrossRef

Irianto A, Austin B (2002a) Probiotics in aquaculture. J Fish Dis 25:633–642CrossRef

Irianto A, Austin B (2002b) Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 25:333–342CrossRef

Kumar R, Mukherjee SC, Ranjan R, Nayak SK (2008) Enhanced innate immune parameters in Labeo rohita (Ham.) following oral administration of Bacillus subtilis. Fish Shellfish Immunol 24:168–172CrossRefPubMed

Lauzon HL, Gudmundsdottir S, Steinarsson A, Oddgeirsson M, Petursdottir SK, Reynisson E, Bjornsdottir R, Gudmundsdottir BK (2010) Effects of bacterial treatment at early stages of Atlantic cod (Gadus morhua L.) on larval survival and development. J Appl Microbiol 108:624–632CrossRefPubMed

Mahious AS, Ollevier F (2005) Probiotics and prebiotics in aquaculture. 1st regional workshop on techniques for enrichment of live food for use in larviculture-2005. AAARC, Urmia, Iran, p 67

Misra CK, Das BK, Mukherjee SC, Pattnaik P (2006) Effect of multiple injections of b-glucan on non-specific immune response and disease resistance in Labeo rohita fingerlings. Fish Shellfish Immunol 20:305–319CrossRefPubMed

Montajami S, Hajiahmadyan M, Vajargah MF, Mirzaie FS, Hosseini SA (2012) Effect of synbiotic (Biomin IMBO) on growth performance and survival rate of Texas cichlid (Herichthys cyanoguttatus) larvae. Global Veterinaria 9:358–361

Naseri S, Khara H, Shakoori M (2013) Effects of probiotics and Fe ion on the growth and survival and body composition of rainbow trout, Oncorhynchus mykiss (Walbaum, 1792) frys. J Appl Anim Res 41:318–325CrossRef

Nayak SK (2010) Probiotics and immunity: a fish perspectives. Fish Shellfish Immunol 29:2–14CrossRefPubMed

Nekoubin H, Gharedashi E, Imanpour MR, Asgharimoghadam A (2012) The influence of synbiotic (Biomin IMBO) on growth factors and survival rate of zebrafish (Danio rerio) Larvae via Supplementation with Biomar. Global Veterinaria 8(5):503–506

Nikoskelainen S, Ouwehand AC, Bylund G, Salminen S, Lilius EM (2003) Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish Shellfish Immunol 15:443–452CrossRefPubMed

Panigrahia A, Kirona V, Puangkaewa J, Kobayashib T, Satoha S, Sugitac H (2005) The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. Aquacult 243:241–254CrossRef

Picchietti S, Fausto AM, Randelli E, Carnevali O, Taddei AR, Buonocore F, Scapigliati G, Abelli L (2009) Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immune-related genes of larval Dicentrarchus labrax (L.). Fish Shellfish Immunol 26:368–376CrossRefPubMed

Ranzani-Paiva MJT, Ishikawa CM, das Eiras AA, Fel izardo NN (2000) Haemotological analysis of ‘chara’ Pseudoplatystoma fasciatum in captivity . Aqua 2000. Responsible aquaculture in the new millennium, Nice, France, 2^6 May 2000, European Aquaculture Society special publication 28, 590 pp

Rehulka J, Minark B, Adamec V, Rehulka E (2005) Investigation of physiological and pathological levels of total plasma protein in rainbow trout (Oncorhynchus mykiss). Aquac Res 36:22–32CrossRef

Ringø E, Gatesoupe FJ (1998) Lactic acid bacteria in fish: a review. Aquacult 160:177–203CrossRef

Ringø E, Olsen R, Gifstad T, Dalmo R, Amlund H, Hemre GL, Bakke AM (2010) Prebiotics in aquaculture: a review. Aquac Nutr 16:117–136CrossRef

Roberfroid M (2007) Prebiotics: the concept revised. J Nutr 137:830S–837SPubMed

Rodriguez-Estrada U, Satoh S, Haga Y, Fushimi H, Sweetman J (2009) Effects of single and combined supplementation of Enterococcus faecalis, mannan oligosaccharide and polyhydrobutyric acid on growth performance and immune response of rainbow trout Oncorhynchus mykiss. Aquacult Sci 57:609–617

Sahu MK, Swarnakumar NS, Sivakumar K, Thangaradjou T, Kannan L (2008) Probiotics in aquaculture: importance and future perspectives. Ind J Microbiol 48:229–308CrossRef

Salinas I, Abelli L, Bertoni F, Picchietti S, Roque A, Furones D, Cuesta A, Meseguer J, Esteban MA (2008) Monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol 25:114–123CrossRefPubMed

Serpunin GG, Likhatchyova OA (1998) Use of the ichthyohaematological studies in ecological monitoring of the reservoirs. Acta Vet Brno 67:339–345CrossRef

Staykov Y (2004) The influence of Bio-Mos on growth rate and immune status of common carp (Cyprinus carpio). In: Alltech’s Second Annual Aquaculture Meeting. Dunboyne, Co. Meath, November, 2004. Oral communication

Vershuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671CrossRef

Walker WA, Duffy LC (1998) Diet and bacterial colonization: role of probiotics and prebiotics. J Nutr Biochem 9:668–675CrossRef

Yazawa K, Imai K, Tamura Z (1978) Oligosaccharides and polysaccharides specifically utilisable by bifdobacteria. Chem Pharm Bull 26:3306–3311CrossRefPubMed

Yousefan M, Amiri MS (2009) A review of the use of prebiotic in aquaculture for fish and shrimp. Afr J Biotechnol 8:7313–7318

Titel: Effects of synbiotic (Biomin IMBO) on haematological and immunological components of Russian sturgeon, Acipenser guldenstadti
verfasst von: Ebrahim Jafarzadeh
Hossein Khara
Mohaddeseh Ahmadnezhad
Publikationsdatum: 01.11.2015
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 6/2015
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-015-2071-6

Springer Medizin

Effects of synbiotic (Biomin IMBO) on haematological and immunological components of Russian sturgeon, Acipenser guldenstadti

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2015

Serum protein profile of hookworm infection in dogs

Immunoexpression of metalloproteinase 14 and tissue inhibitor of metalloproteinase 2 in colorectal carcinomas and lymph node metastases

Clinical aspects, morphometry, and morphology of blood cells of the emu (Dromaius novaehollandiae) reared in captivity

Sub-acute toxicity of lantadenes isolated from Lantana camara leaves in guinea pig animal model

Topical co-administration of flaxseed and pistachio ointment promoted wound healing; evidence for histopathological features

The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie