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14.02.2023 | Original Article

Effects of Unilateral Vagotomy on LPS-Induced Aspiration Pneumonia in Mice

verfasst von: Daisuke Tatsushima, Takaomi Kurioka, Kunio Mizutari, Jun Suzuki, Ryoukichi Ikeda, Takuma Hisaoka, Yasutoshi Koshiba, Hiyori Takahashi, Hikaru Hashimoto, Yukio Katori, Akihiro Shiotani

Erschienen in: Dysphagia | Ausgabe 5/2023

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Abstract

Respiratory-related dysphagia and aspiration pneumonia can be attributed to multiple causes. However, reproduction of multiple factor-related respiratory distress and aspiration pneumonia in a single animal model is challenging. To validate animals with vagal nerve palsy as novel models for severe aspiration pneumonia associated with respiratory distress, we investigated the effects of unilateral vagotomy on the swallowing function and severity of pneumonia after forced aspiration in mice. Unilateral vagotomy was performed in C57BL6 male mice that subsequently underwent evaluation of swallowing function by videofluoroscopic swallow study (VFSS) and histological assessments for aspiration pneumonia induced by lipopolysaccharide (LPS). VFSS examinations demonstrated that unilateral vagotomy did not cause apparent aspiration in mice, but it resulted in a significant loss of body weight (BW) due to decreased oral intake. In addition, when aspiration pneumonia was induced by forced administration of LPS, significantly prolonged BW loss and severe infiltration of inflammatory cells associated with aspiration pneumonia were observed in the mice that underwent unilateral vagotomy. In conclusion, the vagotomized mice showed appropriate characteristics as a model of aspiration pneumonia caused by multiple factors, including the paralysis of vocal fold movement and respiratory distress. This model can help elucidate the pathogenesis of aspiration pneumonia and the treatment methods for the respiration-compromised model.

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Titel: Effects of Unilateral Vagotomy on LPS-Induced Aspiration Pneumonia in Mice
verfasst von: Daisuke Tatsushima
Takaomi Kurioka
Kunio Mizutari
Jun Suzuki
Ryoukichi Ikeda
Takuma Hisaoka
Yasutoshi Koshiba
Hiyori Takahashi
Hikaru Hashimoto
Yukio Katori
Akihiro Shiotani
Publikationsdatum: 14.02.2023
Verlag: Springer US
Erschienen in: Dysphagia / Ausgabe 5/2023
Print ISSN: 0179-051X
Elektronische ISSN: 1432-0460
DOI: https://doi.org/10.1007/s00455-023-10564-3

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Effects of Unilateral Vagotomy on LPS-Induced Aspiration Pneumonia in Mice

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