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International Urology and Nephrology

Erschienen in:

01.03.2008 | Original Article

Effects of wortmannin on phosphorylation of PDK1, GSK3-β, PTEN and expression of Skp2 mRNA after ischemia/reperfusion injury in the mouse kidney

verfasst von: Xiangyi Zheng, Liping Xie, Jie Qin, Huafeng Shen, Zhaodian Chen, Yongfeng Jin

Erschienen in: International Urology and Nephrology | Ausgabe 1/2008

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Abstract

Aim

The aim of this study was to investigate the role of 3-phosphoinositide-dependent protein kinase-1 (PDK1), glycogen synthase kinase-3β (GSK3-β) and phosphatase and tensin homologue deleted on chromosome 10 (PTEN), which are the components of the phosphoinositide 3-kinase (PI3K)/Akt pathway, and expression of S-phase kinase-associated protein 2 (Skp2) messenger RNA (mRNA) in renal ischemia/reperfusion.

Materials and methods

Three experimental groups, sham-operative mice, vehicle-delivered mice and wortmannin-treated ischemia/reperfusion injury (IRI) mice were designed to examine PDK1, GSK3-β and PTEN phosphorylation status, as well as expression of Skp2 mRNA at 30 min, 90 min, 24 h and 48 h of reperfusion after ischemia treatment. Wortmannin or its vehicle was given intraperitoneally 4 h before surgery. Expression of Skp2 mRNA was examined by semiquantitative reverse-transcription PCR, and the components of the PI3K/Akt pathway were detected by western blotting in the IRI kidney.

Results

Phosphorylation of PDK1(Ser241), GSK3-β(Ser9) and PTEN(Ser380) was increased after ischemia/reperfusion in the mouse kidney, and phosphorylation of PDK1 was reduced by wortmannin administration. The renal Skp2 mRNA increased after IRI in mouse, which could be inhibited by wortmannin.

Conclusions

Our primary study suggests that the PI3K/Akt signaling pathway plays an important role in regulating the repair following renal IRI. The Skp2 mRNA increased in the IRI kidney and may be regulated by the PI3K/Akt pathway.

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Titel: Effects of wortmannin on phosphorylation of PDK1, GSK3-β, PTEN and expression of Skp2 mRNA after ischemia/reperfusion injury in the mouse kidney
verfasst von: Xiangyi Zheng
Liping Xie
Jie Qin
Huafeng Shen
Zhaodian Chen
Yongfeng Jin
Publikationsdatum: 01.03.2008
Verlag: Springer Netherlands
Erschienen in: International Urology and Nephrology / Ausgabe 1/2008
Print ISSN: 0301-1623
Elektronische ISSN: 1573-2584
DOI: https://doi.org/10.1007/s11255-007-9215-9

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