nach oben

Clinical Oral Investigations

Erschienen in:

01.01.2024 | Research

Efficacy of injectable platelet-rich fibrin on clinical and biochemical parameters in non-surgical periodontal treatment: a split-mouth randomized controlled trial

verfasst von: Gizem Torumtay Cin, Aysan Lektemur Alpan, Özge Çevik

Erschienen in: Clinical Oral Investigations | Ausgabe 1/2024

Einloggen, um Zugang zu erhalten

Abstract

Objectives

The purpose of this clinical trial was to evaluate the potential clinical and biochemical effects of injectable platelet-rich fibrin (i-PRF) application adjunct to scaling and root planning (ScRp) in deep periodontal pockets.

Materials and methods

In this split-mouth-designed study, 17 patients with 34 deep periodontal pockets were randomly treated with ScRp + i-PRF (test group) and ScRp + saline (control group). Clinical periodontal measurements were recorded at baseline, 1st, 3rd, and 6th months after the treatments. The levels of vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α), and interleukin (IL)-10 in gingival crevicular fluid (GCF) samples were analyzed using the ELISA method at baseline, 7th, and 14th days.

Results

Clinical periodontal parameters showed significant improvements with both treatment modalities. Mean pocket reduction (PD) and clinical attachment (CAL) gain were significantly higher in the test group than in controls at follow-up visits (p < 0.05). In the test group, gingival recession (GR) values were significantly lower compared to the control group. VEGF and IL-10 levels in the test group were significantly higher than in controls at the 14th day, and TNF-α levels were found significantly lower in the test group at the 7th and 14th days.

Conclusions

Especially in the test group, the significant increase in VEGF and IL-10 expressions and the decrease in TNF-α levels may have accelerated the periodontal healing observed in the clinical parameters.

Clinical relevance

The result of the present study demonstrated the beneficial effects of adjunctive i-PRF administration during non-surgical periodontal treatment of deep periodontal pockets.

Clinical trial registration number

NCT05753631

Savage A, Eaton KA, Moles DR, Needleman I (2009) A systematic review of definitions of periodontitis and methods that have been used to identify this disease. J Clin Periodontol 36:458–467. https://doi.org/10.1111/j.1600-051X.2009.01408.xCrossRefPubMed

Slots J (2012) Low-cost periodontal. Periodontology 2000(60):110–137. https://doi.org/10.1111/j.1600-0757.2011.00429.xCrossRef

Ozcan E, Saygun I, Kantarci A, Ozarslanturk S, Serdar MA, Ozgurtas T (2021) The effects of a novel non-invasive application of platelet-rich fibrin on periodontal clinical parameters and gingival crevicular fluid transforming growth factor-beta and collagen-1 levels: a randomized, controlled, clinical study. J Periodontol 92:1252–1261. https://doi.org/10.1002/JPER.20-0713CrossRefPubMed

Heitz-Mayfield LJ, Lang NP (2013) Surgical and nonsurgical periodontal therapy. Learned and unlearned concepts. Periodontol 2000 62:218–231. https://doi.org/10.1111/prd.12008CrossRefPubMed

Jurdziński KT, Potempa J, Grabiec AM (2020) Epigenetic regulation of inflammation in periodontitis: cellular mechanisms and therapeutic potential. Clin Epigenet 12:186. https://doi.org/10.1186/s13148-020-00982-7CrossRef

Pan W, Wang Q, Chen Q (2019) The cytokine network involved in the host immune response to periodontitis. Int J Oral Sci 11:30. https://doi.org/10.1038/s41368-019-0064-zCrossRefPubMedPubMedCentral

Noh MK, Jung M, Kim SH, Lee SR, Park KH, Kim DH, Kim HH, Park YG (2013) Assessment of IL-6, IL-8 and TNF-alpha levels in the gingival tissue of patients with periodontitis. Exp Ther Med 6:847–851. https://doi.org/10.3892/etm.2013.1222CrossRefPubMedPubMedCentral

Uitto VJ (2000) Overall CM and McCulloch C (2003) Proteolytic host cell enzymes in gingival crevice fluid. Periodontology 31:77–104. https://doi.org/10.1034/j.1600-0757.2003.03106.xCrossRef

Bastos MF, Lima JA, Vieira PM, Mestnik MJ, Faveri M, Duarte PM (2009) TNF-alpha and IL-4 levels in generalized aggressive periodontitis subjects. Oral Dis 15:82–87. https://doi.org/10.1111/j.1601-0825.2008.01491.xCrossRefPubMed

10.

Moudi B, Heidari Z, Mahmoudzadeh-Sagheb H, Hashemi M, Metanat M, Khosravi S, Farrokh P (2016) Association between IL-10 gene promoter polymorphisms (-592 A/C, -819 T/C, -1082 A/G) and susceptibility to HBV infection in an Iranian population. Hepat Mon 16:e32427. https://doi.org/10.5812/hepatmon.32427CrossRefPubMedPubMedCentral

11.

Li B, Gurung P, Malireddi RKS, Vogel P, Kanneganti T-D, Geiger TL (2015) IL-10 engages macrophages to shift Th17 cytokine dependency and pathogenicity during T-cell-mediated colitis. Nat Commun 6:6131. https://doi.org/10.1038/ncomms7131CrossRefPubMedADS

12.

Ren B, Feng Q, He S, Li Y, Fan J, Chai G, Liu L, Liu H, Yang C, Wang Y, Liu H, Liu H, Song Y (2021) VEGF as a potential molecular target in periodontitis: a meta-analysis and microarray data validation. J Inflamm 18:18. https://doi.org/10.1186/s12950-021-00281-9CrossRef

13.

Borie E, Olivi DG, Orsi IA, Garlet K, Weber B, Beltran V, Fuentes R (2015) Platelet-rich fibrin application in dentistry: a literature review. Int J Clin Exp Med 8:7922–7929PubMedPubMedCentral

14.

Choukroun J, Ghanaati S (2018) Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients' own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg 44:87–95. https://doi.org/10.1007/s00068-017-0767-9CrossRefPubMed

15.

Jasmine S, Thangavelu A, Krishnamoorthy R, Alshuniaber MA, Alshatwi AA (2021) Cytokine expression pattern and protein-protein interaction network analysis of leucocyte rich platelet rich fibrin and injectable form of platelet rich fibrin. Oral Maxillofac Surg 25:223–229. https://doi.org/10.1007/s10006-020-00899-8CrossRefPubMed

16.

Lei L, Yu Y, Ke T, Sun W, Chen L (2019) The application of three-dimensional printing model and platelet-rich fibrin technology in guided tissue regeneration surgery for severe bone defects. J Oral Implantol 45:35–43. https://doi.org/10.1563/aaid-joi-D-17-00231CrossRefPubMed

17.

Kyyak S, Blatt S, Pabst A, Thiem D, Al-Nawas B, Kammerer PW (2020) Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin - a comparative in vitro study. J Biomater Appl 35:83–96. https://doi.org/10.1177/0885328220914407CrossRefPubMed

18.

Gollapudi M, Bajaj P, Oza RR (2022) Injectable platelet-rich fibrin - a revolution in periodontal regeneration. Cureus 14:e28647. https://doi.org/10.7759/cureus.28647CrossRefPubMedPubMedCentral

19.

Miron RJ, Zhang Y (2018) Autologous liquid platelet rich fibrin: a novel drug delivery system. Acta Biomaterialia 75:35–51. https://doi.org/10.1016/j.actbio.2018.05.021CrossRefPubMed

20.

Farshidfar N, Amiri MA, Jafarpour D, Hamedani S, Niknezhad SV, Tayebi L (2022) The feasibility of injectable PRF (I-PRF) for bone tissue engineering and its application in oral and maxillofacial reconstruction: From bench to chairside. Biomater Adv 134:112557. https://doi.org/10.1016/j.msec.2021.112557CrossRefPubMed

21.

Wend S, Kubesch A, Orlowska A, Al-Maawi S, Zender N, Dias A, Miron RJ, Sader R, Booms P, Kirkpatrick CJ, Choukroun J, Ghanaati S (2017) Reduction of the relative centrifugal force influences cell number and growth factor release within injectable PRF-based matrices. J Mater Sci: Mater Med 28:188. https://doi.org/10.1007/s10856-017-5992-6CrossRefPubMed

22.

Miron RJ, Fujioka-Kobayashi M, Hernandez M, Kandalam U, Zhang Y, Ghanaati S, Choukroun J (2017) Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry? Clin Oral Investig 21:2619–2627. https://doi.org/10.1007/s00784-017-2063-9CrossRefPubMed

23.

Zhang J, Yin C, Zhao Q, Zhao Z, Wang J, Miron RJ, Zhang Y (2020) Anti-inflammation effects of injectable platelet-rich fibrin via macrophages and dendritic cells. J Biomed Mater Res A 108:61–68. https://doi.org/10.1002/jbm.a.36792CrossRefPubMed

24.

Wu B, Mottola G, Schaller M, Upchurch GR Jr, Conte MS (2017) Resolution of vascular injury: Specialized lipid mediators and their evolving therapeutic implications. Mol Aspects Med 58:72–82. https://doi.org/10.1016/j.mam.2017.07.005CrossRefPubMedPubMedCentral

25.

Polimeni G, Xiropaidis AV, Wikesjo UM (2006) Biology and principles of periodontal wound healing/regeneration. Periodontol 2000 41:30–47. https://doi.org/10.1111/j.1600-0757.2006.00157.xCrossRefPubMed

26.

Mohan SP, Jaishangar N, Devy S, Narayanan A, Cherian D, Madhavan SS (2019) Platelet-rich plasma and platelet-rich fibrin in periodontal regeneration: a review. J Pharm Bioallied Sci 11:S126–S130. https://doi.org/10.4103/JPBS.JPBS_41_19CrossRefPubMedPubMedCentral

27.

Arabaci T, Kose O, Albayrak M, Cicek Y, Kizildag A (2017) Advantages of autologous platelet-rich fibrin membrane on gingival crevicular fluid growth factor levels and periodontal healing: a randomized split-mouth clinical study. J Periodontol 88:771–777. https://doi.org/10.1902/jop.2017.160485CrossRefPubMed

28.

Izol BS, Uner DD (2019) A new approach for root surface biomodification using injectable platelet-rich fibrin (I-PRF). Med Sci Monit 25:4744–4750. https://doi.org/10.12659/MSM.915142CrossRefPubMedPubMedCentral

29.

Ozsagir ZB, Saglam E, Sen Yilmaz B, Choukroun J, Tunali M (2020) Injectable platelet-rich fibrin and microneedling for gingival augmentation in thin periodontal phenotype: a randomized controlled clinical trial. J Clin Periodontol 47:489–499. https://doi.org/10.1111/jcpe.13247CrossRefPubMed

30.

Aydinyurt HS, Sancak T, Taskin C, Basbugan Y, Akinci L (2021) Effects of injectable platelet-rich fibrin in experimental periodontitis in rats. Odontology 109:422–432. https://doi.org/10.1007/s10266-020-00557-1CrossRefPubMed

31.

Albonni H, El Abdelah A, Al Hamwi M, Al Hamoui WB, Sawaf H (2021) Clinical effectiveness of a topical subgingival application of injectable platelet-rich fibrin as adjunctive therapy to scaling and root planing: a double-blind, split-mouth, randomized, prospective, comparative controlled trial. Quintessence Int 52:676–685. https://doi.org/10.3290/j.qi.b1492019CrossRefPubMed

32.

Melincovici CS, Bosca AB, Susman S, Marginean M, Mihu C, Istrate M, Moldovan IM, Roman AL, Mihu CM (2018) Vascular endothelial growth factor (VEGF) - key factor in normal and pathological angiogenesis. Rom J Morphol Embryol 59:455–467PubMed

33.

Suthin K, Matsushita K, Machigashira M, Tatsuyama S, Imamura T, Torii M, Izumi Y (2003) Enhanced expression of vascular endothelial growth factor by periodontal pathogens in gingival fibroblasts. J Periodontal Res 38:90–96. https://doi.org/10.1034/j.1600-0765.2003.01646.xCrossRefPubMed

34.

Chapple CC, Kumar RK, Hunter N (2000) Vascular remodelling in chronic inflammatory periodontal disease. J Oral Pathol Med 29:500–506. https://doi.org/10.1034/j.1600-0714.2000.291004.xCrossRefPubMed

35.

Unlu F, Guneri PG, Hekimgil M, Yesilbek B, Boyacioglu H (2003) Expression of vascular endothelial growth factor in human periodontal tissues: comparison of healthy and diabetic patients. J Periodontol 74:181–187. https://doi.org/10.1902/jop.2003.74.2.181CrossRefPubMed

36.

Fernandez-Medina T, Vaquette C, Ivanovski S (2019) Systematic comparison of the effect of four clinical-grade platelet rich hemoderivatives on osteoblast behaviour. Int J Mol Sci 20. https://doi.org/10.3390/ijms20246243

37.

Dohle E, El Bagdadi K, Sader R, Choukroun J, James Kirkpatrick C, Ghanaati S (2018) Platelet-rich fibrin-based matrices to improve angiogenesis in an in vitro co-culture model for bone tissue engineering. J Tissue Eng Regen Med 12:598–610. https://doi.org/10.1002/term.2475CrossRefPubMed

38.

Zhao B (2017) TNF and bone remodeling. Curr Osteoporos Rep 15:126–134. https://doi.org/10.1007/s11914-017-0358-zCrossRefPubMedPubMedCentral

39.

Fujihara R, Usui M, Yamamoto G, Nishii K, Tsukamoto Y, Okamatsu Y, Sato T, Asou Y, Nakashima K, Yamamoto M (2014) Tumor necrosis factor-alpha enhances RANKL expression in gingival epithelial cells via protein kinase A signaling. J Periodontal Res 49:508–517. https://doi.org/10.1111/jre.12131CrossRefPubMed

40.

Osta B, Benedetti G, Miossec P (2014) Classical and paradoxical effects of TNF-alpha on bone homeostasis. Front Immunol 5:48. https://doi.org/10.3389/fimmu.2014.00048CrossRefPubMedPubMedCentral

41.

Algate K, Haynes DR, Bartold PM, Crotti TN, Cantley MD (2016) The effects of tumour necrosis factor-alpha on bone cells involved in periodontal alveolar bone loss; osteoclasts, osteoblasts and osteocytes. J Periodontal Res 51:549–566. https://doi.org/10.1111/jre.12339CrossRefPubMed

42.

Murai M, Turovskaya O, Kim G, Madan R, Karp CL, Cheroutre H, Kronenberg M (2009) Interleukin 10 acts on regulatory T cells to maintain expression of the transcription factor Foxp3 and suppressive function in mice with colitis. Nat Immunol 10:1178–1184. https://doi.org/10.1038/ni.1791CrossRefPubMedPubMedCentral

43.

Park-Min KH, Ji JD, Antoniv T, Reid AC, Silver RB, Humphrey MB, Nakamura M, Ivashkiv LB (2009) IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression. J Immunol 183:2444–2455. https://doi.org/10.4049/jimmunol.0804165CrossRefPubMed

44.

Moore KW, de Waal MR, Coffman RL, O’Garra A (2001) Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol 19:683–765. https://doi.org/10.1146/annurev.immunol.19.1.683CrossRefPubMed

45.

Ernst CW, Lee JE, Nakanishi T, Karimbux NY, Rezende TM, Stashenko P, Seki M, Taubman MA, Kawai T (2007) Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease. Clin Exp Immunol 148:271–280. https://doi.org/10.1111/j.1365-2249.2006.03318.xCrossRefPubMedPubMedCentral

46.

Maheshwari S, Grover V, Gupta J, Kumar N, Jain A (2021) Effect of non-surgical periodontal therapy on the serum and crevicular fluid interleukin-10 levels in chronic periodontitis - a systematic review and meta-analysis. Int J Health Sci (Qassim) 15:34–46PubMed

47.

Goutoudi P, Diza E, Arvanitidou M (2004) Effect of periodontal therapy on crevicular fluid interleukin-1beta and interleukin-10 levels in chronic periodontitis. J Dent 32:511–520. https://doi.org/10.1016/j.jdent.2004.04.003CrossRefPubMed

48.

Varela HA, Souza JCM, Nascimento RM, Araujo RF Jr, Vasconcelos RC, Cavalcante RS, Guedes PM, Araujo AA (2019) Injectable platelet rich fibrin: cell content, morphological, and protein characterization. Clin Oral Investig 23:1309–1318. https://doi.org/10.1007/s00784-018-2555-2CrossRefPubMed

Titel: Efficacy of injectable platelet-rich fibrin on clinical and biochemical parameters in non-surgical periodontal treatment: a split-mouth randomized controlled trial
verfasst von: Gizem Torumtay Cin
Aysan Lektemur Alpan
Özge Çevik
Publikationsdatum: 01.01.2024
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 1/2024
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-023-05447-8

Bestellen Sie unseren kostenlosen Newsletter Update Zahnmedizin und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Klimawandel und Gesundheit: Was Sie in der Hausarztpraxis wissen müssen und tun können

Springer Medizin

Efficacy of injectable platelet-rich fibrin on clinical and biochemical parameters in non-surgical periodontal treatment: a split-mouth randomized controlled trial

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Clinical trial registration number

Neu im Fachgebiet Zahnmedizin

Darf man die Behandlung eines Neonazis ablehnen?

Ein Drittel der jungen Ärztinnen und Ärzte erwägt abzuwandern

Endlich: Zi zeigt, mit welchen PVS Praxen zufrieden sind

Parodontalbehandlung verbessert Prognose bei Katheterablation

Newsletter

Klimawandel und Gesundheit: Was Sie in der Hausarztpraxis wissen müssen und tun können

Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Clinical trial registration number

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2024

Facts and trends in dental antibiotic and analgesic prescriptions in Germany, 2012–2021

A deep learning and radiomics fusion model based on contrast-enhanced computer tomography improves preoperative identification of cervical lymph node metastasis of oral squamous cell carcinoma

Correction to: A comparative study of biological properties of three root canal sealers

Effects of pain associated with orthodontic tooth movement on tactile sensation of periodontal ligaments

Influence of crestal and sub-crestal implant position on development of peri-implant diseases: a 5-year retrospective analysis

Clinical performance of a glass-hybrid system in comparison with a resin composite in two-surface class II restorations: a 5-year randomised multi-centre study

Neu im Fachgebiet Zahnmedizin

Darf man die Behandlung eines Neonazis ablehnen?

Ein Drittel der jungen Ärztinnen und Ärzte erwägt abzuwandern

Endlich: Zi zeigt, mit welchen PVS Praxen zufrieden sind

Parodontalbehandlung verbessert Prognose bei Katheterablation

Newsletter