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29.11.2018 | Original Article

Endometrial Cancer Spheres Show Cancer Stem Cells Phenotype and Preference for Oxidative Metabolism

verfasst von: Maria João Carvalho, Mafalda Laranjo, Ana Margarida Abrantes, João Casalta-Lopes, Daniela Sarmento-Santos, Tânia Costa, Beatriz Serambeque, Nuno Almeida, Telmo Gonçalves, Catarina Mamede, João Encarnação, Rui Oliveira, Artur Paiva, Rui de Carvalho, Filomena Botelho, Carlos Oliveira

Erschienen in: Pathology & Oncology Research | Ausgabe 3/2019

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Abstract

This study aimed to characterize endometrial cancer regarding cancer stem cells (CSC) markers, regulatory and differentiation pathways, tumorigenicity and glucose metabolism. Endometrial cancer cell line ECC1 was submitted to sphere forming protocols. The first spheres generation (ES1) was cultured in adherent conditions (G1). This procedure was repeated and was obtained generations of spheres (ES1, ES2 and ES3) and spheres-derived cells in adherent conditions (G1, G2 and G3). Populations were characterized regarding CD133, CD24, CD44, aldehyde dehydrogenase (ALDH), hormonal receptors, HER2, P53 and β-catenin, fluorine-18 fluorodeoxyglucose ([¹⁸F]FDG) uptake and metabolism by NMR spectroscopy. An heterotopic model evaluated differential tumor growth. The spheres self-renewal was higher in ES3. The putative CSC markers CD133, CD44 and ALDH expression were higher in spheres. The expression of estrogen receptor (ER)α and P53 decreased in spheres, ERβ and progesterone receptor had no significant changes and β-catenin showed a tendency to increase. There was a higher ¹⁸F-FDG uptake in spheres, which also showed a lower lactate production and an oxidative cytosol status. The tumorigenesis in vivo showed an earlier growth of tumours derived from ES3. Endometrial spheres presented self-renewal and differentiation capacity, expressed CSC markers and an undifferentiated phenotype, showing preference for oxidative metabolism.

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Titel: Endometrial Cancer Spheres Show Cancer Stem Cells Phenotype and Preference for Oxidative Metabolism
verfasst von: Maria João Carvalho
Mafalda Laranjo
Ana Margarida Abrantes
João Casalta-Lopes
Daniela Sarmento-Santos
Tânia Costa
Beatriz Serambeque
Nuno Almeida
Telmo Gonçalves
Catarina Mamede
João Encarnação
Rui Oliveira
Artur Paiva
Rui de Carvalho
Filomena Botelho
Carlos Oliveira
Publikationsdatum: 29.11.2018
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 3/2019
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-018-0535-0

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Endometrial Cancer Spheres Show Cancer Stem Cells Phenotype and Preference for Oxidative Metabolism

Abstract

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