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Open Access 21.11.2017 | Review

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

verfasst von: Seiichi Sakamoto, Waraporn Putalun, Sornkanok Vimolmangkang, Waranyoo Phoolcharoen, Yukihiro Shoyama, Hiroyuki Tanaka, Satoshi Morimoto

Erschienen in: Journal of Natural Medicines | Ausgabe 1/2018

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Abstract

Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen–antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies by drug monitoring, and the quality control of commercially available products. Berson and Yalow were the first to develop an immunoassay, known as radioimmunoassay (RIA), for detecting endogenous plasma insulin [1], a development for which Yalow was awarded the Nobel Prize in Physiology or Medicine in 1977. Even today, after half a century, immunoassays are widely utilized with some modifications from the originally proposed system, e.g., radioisotopes have been replaced with enzymes because of safety concerns regarding the use of radioactivity, which is referred to as enzyme immunoassay/enzyme-linked immunosorbent assay (ELISA). In addition, progress has been made in ELISA with the recent advances in recombinant DNA technology, leading to increase in the range of antibodies, probes, and even systems. This review article describes ELISA and its applications for the detection of plant secondary metabolites.

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Titel: Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites
verfasst von: Seiichi Sakamoto
Waraporn Putalun
Sornkanok Vimolmangkang
Waranyoo Phoolcharoen
Yukihiro Shoyama
Hiroyuki Tanaka
Satoshi Morimoto
Publikationsdatum: 21.11.2017
Verlag: Springer Singapore
Erschienen in: Journal of Natural Medicines / Ausgabe 1/2018
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-017-1144-z

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

Abstract

Live-Webinar "Chronische Unterbauch- und Viszeralschmerzen in der Praxis managen"

Springer Medizin

Abstract

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